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[Effect of moxibustion at "Shenque" (CV8) and "Zusanli" (ST36) on expression of adiponectin and adiponectin receptor in Alzheimer's disease rats].
Zhen Ci Yan Jiu 2019; 44(6):419-23ZC

Abstract

OBJECTIVE

To observe the effect of moxibustion on serum adiponectin content, and expression of adiponectin and adiponectin receptor in adipose tissue in Alzheimer's disease (AD) rats, so as to explore its mechanism underlying improvement of AD.

METHODS

Fifty male SD rats were randomly divided into control, model, Shenque (CV8), Zusanli (ST36) and CV8+ST36 groups (n=10 in each group). The AD model was established by intraperitoneal injection of D-galactose (400 mg•kg-1•d-1) for 5 weeks and scopolamine hydrobromide (3 mg •kg-1•d-1) for 2 weeks. Moxibustion was applied to CV8, ST36 and CV8+ST36 respectively for 3 moxa-cones every time, once daily for 5 weeks. Morris water maze tests were used to assess the rats' learning-memory ability. The contents of serum adiponectin were assayed using ELISA, and the expression of adiponectin and adiponectin receptor in the adipose tissue was detected by quantitative real-time PCR and Western blot, separately.

RESULTS

Following modeling, the average escape latency of Morris water maze tests was significantly prolonged (P<0.05), the content of serum adiponectin and the expression level of adiponectin mRNA in adipose tissue were significantly decreased (P<0.01), the expression of adiponectin receptor protein significantly decreased in the model group relevant to the control group (P<0.01). After the intervention, the average escape latency was significantly shortened (P<0.05), the decreased serum adiponectin content and adiponectin mRNA expression, and the decreased adiponectin receptor protein expression in adipose tissue were all reversed in the 3 treatment groups (P<0.01,P<0.05). No significant differences were found among the three moxibustion groups in the above indexes (P>0.05).

CONCLUSION

Moxibustion at CV8, ST36 and CV8+ST36 is effective in up-regulating serum adiponectin content,adiponectin mRNA expression and adiponectin receptor protein expression in adipose tissue, which may provide evidence for clinical election of acupoints.

Authors+Show Affiliations

School of Acupuncture-moxibustion and Massage, Guangxi University of Traditional Chinese Medicine, Nanning 530001, China.School of Acupuncture-moxibustion and Massage, Guangxi University of Traditional Chinese Medicine, Nanning 530001, China.School of Acupuncture-moxibustion and Massage, Guangxi University of Traditional Chinese Medicine, Nanning 530001, China.School of Acupuncture-moxibustion and Massage, Guangxi University of Traditional Chinese Medicine, Nanning 530001, China.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

31368264

Citation

Zhao, Cai-Jiao, et al. "[Effect of Moxibustion at "Shenque" (CV8) and "Zusanli" (ST36) On Expression of Adiponectin and Adiponectin Receptor in Alzheimer's Disease Rats]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 44, no. 6, 2019, pp. 419-23.
Zhao CJ, Huang YZ, Jiang YF, et al. [Effect of moxibustion at "Shenque" (CV8) and "Zusanli" (ST36) on expression of adiponectin and adiponectin receptor in Alzheimer's disease rats]. Zhen Ci Yan Jiu. 2019;44(6):419-23.
Zhao, C. J., Huang, Y. Z., Jiang, Y. F., & Lu, M. J. (2019). [Effect of moxibustion at "Shenque" (CV8) and "Zusanli" (ST36) on expression of adiponectin and adiponectin receptor in Alzheimer's disease rats]. Zhen Ci Yan Jiu = Acupuncture Research, 44(6), pp. 419-23. doi:10.13702/j.1000-0607.180464.
Zhao CJ, et al. [Effect of Moxibustion at "Shenque" (CV8) and "Zusanli" (ST36) On Expression of Adiponectin and Adiponectin Receptor in Alzheimer's Disease Rats]. Zhen Ci Yan Jiu. 2019 Jun 25;44(6):419-23. PubMed PMID: 31368264.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of moxibustion at "Shenque" (CV8) and "Zusanli" (ST36) on expression of adiponectin and adiponectin receptor in Alzheimer's disease rats]. AU - Zhao,Cai-Jiao, AU - Huang,Yi-Zhu, AU - Jiang,Yun-Fang, AU - Lu,Mei-Jing, PY - 2019/8/2/entrez PY - 2019/8/2/pubmed PY - 2019/8/8/medline KW - Adiponectin KW - Adiponectin receptor KW - Adipose tissue KW - Alzheimer’s disease KW - Moxibustion SP - 419 EP - 23 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 44 IS - 6 N2 - OBJECTIVE: To observe the effect of moxibustion on serum adiponectin content, and expression of adiponectin and adiponectin receptor in adipose tissue in Alzheimer's disease (AD) rats, so as to explore its mechanism underlying improvement of AD. METHODS: Fifty male SD rats were randomly divided into control, model, Shenque (CV8), Zusanli (ST36) and CV8+ST36 groups (n=10 in each group). The AD model was established by intraperitoneal injection of D-galactose (400 mg•kg-1•d-1) for 5 weeks and scopolamine hydrobromide (3 mg •kg-1•d-1) for 2 weeks. Moxibustion was applied to CV8, ST36 and CV8+ST36 respectively for 3 moxa-cones every time, once daily for 5 weeks. Morris water maze tests were used to assess the rats' learning-memory ability. The contents of serum adiponectin were assayed using ELISA, and the expression of adiponectin and adiponectin receptor in the adipose tissue was detected by quantitative real-time PCR and Western blot, separately. RESULTS: Following modeling, the average escape latency of Morris water maze tests was significantly prolonged (P<0.05), the content of serum adiponectin and the expression level of adiponectin mRNA in adipose tissue were significantly decreased (P<0.01), the expression of adiponectin receptor protein significantly decreased in the model group relevant to the control group (P<0.01). After the intervention, the average escape latency was significantly shortened (P<0.05), the decreased serum adiponectin content and adiponectin mRNA expression, and the decreased adiponectin receptor protein expression in adipose tissue were all reversed in the 3 treatment groups (P<0.01,P<0.05). No significant differences were found among the three moxibustion groups in the above indexes (P>0.05). CONCLUSION: Moxibustion at CV8, ST36 and CV8+ST36 is effective in up-regulating serum adiponectin content,adiponectin mRNA expression and adiponectin receptor protein expression in adipose tissue, which may provide evidence for clinical election of acupoints. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/31368264/[Effect_of_moxibustion_at_"Shenque"_(CV8)_and_"Zusanli"_(ST36)_on_expression_of_adiponectin_and_adiponectin_receptor_in_Alzheimer's_disease_rats] L2 - https://medlineplus.gov/alzheimersdisease.html DB - PRIME DP - Unbound Medicine ER -