Tags

Type your tag names separated by a space and hit enter

[Acupoint application of herbal paste relieves symptoms and naso-mucosal inflammatory response by down-regulating serum TGF-β1 in allergic rhinitis rats].
Zhen Ci Yan Jiu 2019; 44(6):430-3ZC

Abstract

OBJECTIVE

To observe the effect of acupoint application of herbal paste on symptoms of allergic rhinitis (AR), serum immunoglobulin E (IgE) and transforming growth factor beta 1 (TGF-β1) level, and number of nasal eosinophils (EOS) in rats with AR, so as to explore its underlying mechanisms.

METHODS

Forty male Wistar rats were randomly divided into normal control, model, medication and acupoint application groups (n=10 rats per group). The AR model was established by intraperitoneal (i.p.) injection of mixture solution of ovalbumin, aluminum hydroxide and normal saline (once every other day, for 7 times), and nasal drip plus spray inhalation of ovalbumin (on the following day of i.p., once daily for 9 days). For acupoint application, the prepared herbal paste (containing White Mustard Seed, Rhizoma Corydalis, unprocessed Radix Kansui, Herba Asari and ginger juice) was applied to bilateral "Feishu" (BL13), "Pishu" (BL20) and "Shenshu" (BL23) for 2 h, once every other day for 7 times. The rats in the medication group were given Fluticasone Propionate nasal spray daily for 14 days. Scores of nasal itching, sneezing and nasal discharge on the day after modeling and the ending of the intervention were used to evaluate behavioral changes. Enzyme linked immunosorbent assay (ELISA) was used to detect the levels of serum IgE and TGF-β1, and the infiltration state of EOS in the nasal mucosa tissue was observed under light microscope after HE staining.

RESULTS

After modeling and compared with the normal control group, the behavioral scores and the levels of serum IgE and TGF-β1 were significantly higher (P<0.05), and the infiltration state of EOS got worse. Compared with the model group, the increased behavioral score and serum IgE and TGF-β1 levels were evidently suppressed (P<0.05) and EOS infiltration severity in the nasal mucosa was obviously milder in both medication and acupoint application groups. No significant differences were found between the medication and acupoint application groups in behavioral score and serum IgE and TGF-β1 levels (P>0.05).

CONCLUSION

Acupoint application can improve the symptoms of AR rats, which may be associated with its effect in down-regulating the levels of serum IgE and TGF-β1.

Authors+Show Affiliations

Department of Acupuncture and Moxibustion, The First Affiliated Hospital of Zhejiang University of Chinese Medicine, Hangzhou 310006, China; The First School of Clinical Medicine of Zhejiang University of Chinese Medicine, Hangzhou 310053.The First School of Clinical Medicine of Zhejiang University of Chinese Medicine, Hangzhou 310053.The First School of Clinical Medicine of Zhejiang University of Chinese Medicine, Hangzhou 310053.The First School of Clinical Medicine of Zhejiang University of Chinese Medicine, Hangzhou 310053.Department of Acupuncture and Moxibustion, The First Affiliated Hospital of Zhejiang University of Chinese Medicine, Hangzhou 310006, China; The First School of Clinical Medicine of Zhejiang University of Chinese Medicine, Hangzhou 310053.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

31368266

Citation

Chen, Shan, et al. "[Acupoint Application of Herbal Paste Relieves Symptoms and Naso-mucosal Inflammatory Response By Down-regulating Serum TGF-β1 in Allergic Rhinitis Rats]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 44, no. 6, 2019, pp. 430-3.
Chen S, Jin YT, Zhu ZY, et al. [Acupoint application of herbal paste relieves symptoms and naso-mucosal inflammatory response by down-regulating serum TGF-β1 in allergic rhinitis rats]. Zhen Ci Yan Jiu. 2019;44(6):430-3.
Chen, S., Jin, Y. T., Zhu, Z. Y., Wu, L. T., & Xuan, L. H. (2019). [Acupoint application of herbal paste relieves symptoms and naso-mucosal inflammatory response by down-regulating serum TGF-β1 in allergic rhinitis rats]. Zhen Ci Yan Jiu = Acupuncture Research, 44(6), pp. 430-3. doi:10.13702/j.1000-0607.180369.
Chen S, et al. [Acupoint Application of Herbal Paste Relieves Symptoms and Naso-mucosal Inflammatory Response By Down-regulating Serum TGF-β1 in Allergic Rhinitis Rats]. Zhen Ci Yan Jiu. 2019 Jun 25;44(6):430-3. PubMed PMID: 31368266.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Acupoint application of herbal paste relieves symptoms and naso-mucosal inflammatory response by down-regulating serum TGF-β1 in allergic rhinitis rats]. AU - Chen,Shan, AU - Jin,Yu-Tong, AU - Zhu,Zheng-Yang, AU - Wu,Ling-Tao, AU - Xuan,Li-Hua, PY - 2019/8/2/entrez PY - 2019/8/2/pubmed PY - 2019/8/8/medline KW - Acupoint application KW - Allergic rhinitis KW - Inflammatory regulation KW - TGF-β1 SP - 430 EP - 3 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 44 IS - 6 N2 - OBJECTIVE: To observe the effect of acupoint application of herbal paste on symptoms of allergic rhinitis (AR), serum immunoglobulin E (IgE) and transforming growth factor beta 1 (TGF-β1) level, and number of nasal eosinophils (EOS) in rats with AR, so as to explore its underlying mechanisms. METHODS: Forty male Wistar rats were randomly divided into normal control, model, medication and acupoint application groups (n=10 rats per group). The AR model was established by intraperitoneal (i.p.) injection of mixture solution of ovalbumin, aluminum hydroxide and normal saline (once every other day, for 7 times), and nasal drip plus spray inhalation of ovalbumin (on the following day of i.p., once daily for 9 days). For acupoint application, the prepared herbal paste (containing White Mustard Seed, Rhizoma Corydalis, unprocessed Radix Kansui, Herba Asari and ginger juice) was applied to bilateral "Feishu" (BL13), "Pishu" (BL20) and "Shenshu" (BL23) for 2 h, once every other day for 7 times. The rats in the medication group were given Fluticasone Propionate nasal spray daily for 14 days. Scores of nasal itching, sneezing and nasal discharge on the day after modeling and the ending of the intervention were used to evaluate behavioral changes. Enzyme linked immunosorbent assay (ELISA) was used to detect the levels of serum IgE and TGF-β1, and the infiltration state of EOS in the nasal mucosa tissue was observed under light microscope after HE staining. RESULTS: After modeling and compared with the normal control group, the behavioral scores and the levels of serum IgE and TGF-β1 were significantly higher (P<0.05), and the infiltration state of EOS got worse. Compared with the model group, the increased behavioral score and serum IgE and TGF-β1 levels were evidently suppressed (P<0.05) and EOS infiltration severity in the nasal mucosa was obviously milder in both medication and acupoint application groups. No significant differences were found between the medication and acupoint application groups in behavioral score and serum IgE and TGF-β1 levels (P>0.05). CONCLUSION: Acupoint application can improve the symptoms of AR rats, which may be associated with its effect in down-regulating the levels of serum IgE and TGF-β1. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/31368266/[Acupoint_application_of_herbal_paste_relieves_symptoms_and_naso_mucosal_inflammatory_response_by_down_regulating_serum_TGF_β1_in_allergic_rhinitis_rats]_ DB - PRIME DP - Unbound Medicine ER -