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Multifactorial and Species-Specific Feedback Regulation of the RNA Surveillance Pathway Nonsense-Mediated Decay in Plants.
Plant Cell Physiol. 2019 Sep 01; 60(9):1986-1999.PC

Abstract

Nonsense-mediated decay (NMD) is an RNA surveillance mechanism that detects aberrant transcript features and triggers degradation of erroneous as well as physiological RNAs. Originally considered to be constitutive, NMD is now recognized to be tightly controlled in response to inherent signals and diverse stresses. To gain a better understanding of NMD regulation and its functional implications, we systematically examined feedback control of the central NMD components in two dicot and one monocot species. On the basis of the analysis of transcript features, turnover rates and steady-state levels, up-frameshift (UPF) 1, UPF3 and suppressor of morphological defects on genitalia (SMG) 7, but not UPF2, are under feedback control in both dicots. In the monocot investigated in this study, only SMG7 was slightly induced upon NMD inhibition. The detection of the endogenous NMD factor proteins in Arabidopsis thaliana substantiated a negative correlation between NMD activity and SMG7 amounts. Furthermore, evidence was provided that SMG7 is required for the dephosphorylation of UPF1. Our comprehensive and comparative study of NMD feedback control in plants reveals complex and species-specific attenuation of this RNA surveillance pathway, with critical implications for the numerous functions of NMD in physiology and stress responses.

Authors+Show Affiliations

Center for Plant Molecular Biology (ZMBP), University of T�bingen, Auf der Morgenstelle, 32 T�bingen, Germany.Center for Plant Molecular Biology (ZMBP), University of T�bingen, Auf der Morgenstelle, 32 T�bingen, Germany.Center for Plant Molecular Biology (ZMBP), University of T�bingen, Auf der Morgenstelle, 32 T�bingen, Germany.Center for Plant Molecular Biology (ZMBP), University of T�bingen, Auf der Morgenstelle, 32 T�bingen, Germany.Center for Plant Molecular Biology (ZMBP), University of T�bingen, Auf der Morgenstelle, 32 T�bingen, Germany.Center for Plant Molecular Biology (ZMBP), University of T�bingen, Auf der Morgenstelle, 32 T�bingen, Germany.Center for Plant Molecular Biology (ZMBP), University of T�bingen, Auf der Morgenstelle, 32 T�bingen, Germany.Center for Plant Molecular Biology (ZMBP), University of T�bingen, Auf der Morgenstelle, 32 T�bingen, Germany. Institute for Molecular Physiology (imP), University of Mainz, Johannes von M�ller-Weg 6, Mainz, Germany.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31368494

Citation

Kesarwani, Anil K., et al. "Multifactorial and Species-Specific Feedback Regulation of the RNA Surveillance Pathway Nonsense-Mediated Decay in Plants." Plant & Cell Physiology, vol. 60, no. 9, 2019, pp. 1986-1999.
Kesarwani AK, Lee HC, Ricca PG, et al. Multifactorial and Species-Specific Feedback Regulation of the RNA Surveillance Pathway Nonsense-Mediated Decay in Plants. Plant Cell Physiol. 2019;60(9):1986-1999.
Kesarwani, A. K., Lee, H. C., Ricca, P. G., Sullivan, G., Faiss, N., Wagner, G., Wunderling, A., & Wachter, A. (2019). Multifactorial and Species-Specific Feedback Regulation of the RNA Surveillance Pathway Nonsense-Mediated Decay in Plants. Plant & Cell Physiology, 60(9), 1986-1999. https://doi.org/10.1093/pcp/pcz141
Kesarwani AK, et al. Multifactorial and Species-Specific Feedback Regulation of the RNA Surveillance Pathway Nonsense-Mediated Decay in Plants. Plant Cell Physiol. 2019 Sep 1;60(9):1986-1999. PubMed PMID: 31368494.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Multifactorial and Species-Specific Feedback Regulation of the RNA Surveillance Pathway Nonsense-Mediated Decay in Plants. AU - Kesarwani,Anil K, AU - Lee,Hsin-Chieh, AU - Ricca,Patrizia G, AU - Sullivan,Gabriele, AU - Faiss,Natalie, AU - Wagner,Gabriele, AU - Wunderling,Anna, AU - Wachter,Andreas, PY - 2018/11/30/received PY - 2019/07/06/accepted PY - 2019/8/2/pubmed PY - 2020/1/3/medline PY - 2019/8/2/entrez KW - Arabidopsis thaliana KW - Gene regulation KW - NMD KW - Nonsense-mediated decay KW - Posttranscriptional KW - RNA KW - Surveillance SP - 1986 EP - 1999 JF - Plant & cell physiology JO - Plant Cell Physiol. VL - 60 IS - 9 N2 - Nonsense-mediated decay (NMD) is an RNA surveillance mechanism that detects aberrant transcript features and triggers degradation of erroneous as well as physiological RNAs. Originally considered to be constitutive, NMD is now recognized to be tightly controlled in response to inherent signals and diverse stresses. To gain a better understanding of NMD regulation and its functional implications, we systematically examined feedback control of the central NMD components in two dicot and one monocot species. On the basis of the analysis of transcript features, turnover rates and steady-state levels, up-frameshift (UPF) 1, UPF3 and suppressor of morphological defects on genitalia (SMG) 7, but not UPF2, are under feedback control in both dicots. In the monocot investigated in this study, only SMG7 was slightly induced upon NMD inhibition. The detection of the endogenous NMD factor proteins in Arabidopsis thaliana substantiated a negative correlation between NMD activity and SMG7 amounts. Furthermore, evidence was provided that SMG7 is required for the dephosphorylation of UPF1. Our comprehensive and comparative study of NMD feedback control in plants reveals complex and species-specific attenuation of this RNA surveillance pathway, with critical implications for the numerous functions of NMD in physiology and stress responses. SN - 1471-9053 UR - https://www.unboundmedicine.com/medline/citation/31368494/Multifactorial_and_Species_Specific_Feedback_Regulation_of_the_RNA_Surveillance_Pathway_Nonsense_Mediated_Decay_in_Plants_ L2 - https://academic.oup.com/pcp/article-lookup/doi/10.1093/pcp/pcz141 DB - PRIME DP - Unbound Medicine ER -