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Luteolin exhibits anti-breast cancer property through up-regulating miR-203.
Artif Cells Nanomed Biotechnol 2019; 47(1):3265-3271AC

Abstract

Luteolin is a representative of natural flavonoid that has anti-tumour properties. This study designed to check its impact on breast cancer and the underlying mechanisms. MDA-MB-453 and MCF-7 cells were administrated with luteolin and the following techniques were carried out: CCK-8 assay, FITC-PI double-staining and Western blot. qRT-PCR analysis was utilized to see the effects of luteolin on miR-203 expression. Besides, miR-203 expression was silenced by transfection with specific inhibitor. Luteolin remarkably declined MDA-MB-453 and MCF-7 cells viability and accelerated apoptosis which accompanied by Bax up-regulation, Bcl-2 down-regulation and Caspase-3 cleavage. Also, luteolin impeded TGFβ1-induced EMT, as evidenced by the decreased levels of Vimentin, Zeb1 and N-cadherin, as well as the increased level of E-cadherin. miR-203 was highly expressed in 22 pair of breast cancer tissues than the matched paracancerous tissues. Luteolin could elevate miR-203 level. Besides, luteolin's anti-tumour effects were partially eliminated by miR-203 silence. Further, luteolin inhibited Ras/Raf/MEK/ERK signalling, while the inhibitory effects were flattened by miR-203 silence. Luteolin significantly reduced breast cancer cells growth and EMT. Luteolin exerted its anti-tumour effects possibly involved the elevated expression of miR-203 and the inhibited Ras/Raf/MEK/ERK signalling.

Authors+Show Affiliations

a Department of Galactophore, Linyi Central Hospital , Linyi , PR China.a Department of Galactophore, Linyi Central Hospital , Linyi , PR China.a Department of Galactophore, Linyi Central Hospital , Linyi , PR China.a Department of Galactophore, Linyi Central Hospital , Linyi , PR China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31368817

Citation

Gao, Guanglei, et al. "Luteolin Exhibits Anti-breast Cancer Property Through Up-regulating MiR-203." Artificial Cells, Nanomedicine, and Biotechnology, vol. 47, no. 1, 2019, pp. 3265-3271.
Gao G, Ge R, Li Y, et al. Luteolin exhibits anti-breast cancer property through up-regulating miR-203. Artif Cells Nanomed Biotechnol. 2019;47(1):3265-3271.
Gao, G., Ge, R., Li, Y., & Liu, S. (2019). Luteolin exhibits anti-breast cancer property through up-regulating miR-203. Artificial Cells, Nanomedicine, and Biotechnology, 47(1), pp. 3265-3271. doi:10.1080/21691401.2019.1646749.
Gao G, et al. Luteolin Exhibits Anti-breast Cancer Property Through Up-regulating MiR-203. Artif Cells Nanomed Biotechnol. 2019;47(1):3265-3271. PubMed PMID: 31368817.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Luteolin exhibits anti-breast cancer property through up-regulating miR-203. AU - Gao,Guanglei, AU - Ge,Rongli, AU - Li,Yuzhou, AU - Liu,Shengcui, PY - 2019/8/2/entrez KW - MCF-7 cell KW - MDA-MB-453 cell KW - Ras/Raf/MEK/ERK signalling KW - apoptosis KW - epithelial-mesenchymal transition (EMT) SP - 3265 EP - 3271 JF - Artificial cells, nanomedicine, and biotechnology JO - Artif Cells Nanomed Biotechnol VL - 47 IS - 1 N2 - Luteolin is a representative of natural flavonoid that has anti-tumour properties. This study designed to check its impact on breast cancer and the underlying mechanisms. MDA-MB-453 and MCF-7 cells were administrated with luteolin and the following techniques were carried out: CCK-8 assay, FITC-PI double-staining and Western blot. qRT-PCR analysis was utilized to see the effects of luteolin on miR-203 expression. Besides, miR-203 expression was silenced by transfection with specific inhibitor. Luteolin remarkably declined MDA-MB-453 and MCF-7 cells viability and accelerated apoptosis which accompanied by Bax up-regulation, Bcl-2 down-regulation and Caspase-3 cleavage. Also, luteolin impeded TGFβ1-induced EMT, as evidenced by the decreased levels of Vimentin, Zeb1 and N-cadherin, as well as the increased level of E-cadherin. miR-203 was highly expressed in 22 pair of breast cancer tissues than the matched paracancerous tissues. Luteolin could elevate miR-203 level. Besides, luteolin's anti-tumour effects were partially eliminated by miR-203 silence. Further, luteolin inhibited Ras/Raf/MEK/ERK signalling, while the inhibitory effects were flattened by miR-203 silence. Luteolin significantly reduced breast cancer cells growth and EMT. Luteolin exerted its anti-tumour effects possibly involved the elevated expression of miR-203 and the inhibited Ras/Raf/MEK/ERK signalling. SN - 2169-141X UR - https://www.unboundmedicine.com/medline/citation/31368817/Luteolin_exhibits_anti-breast_cancer_property_through_up-regulating_miR-203 L2 - http://www.tandfonline.com/doi/full/10.1080/21691401.2019.1646749 DB - PRIME DP - Unbound Medicine ER -