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Developmental validation of the Huaxia™ Platinum PCR amplification kit: A 6-dye multiplex direct amplification assay designed for Chinese reference samples.
Forensic Sci Int Genet. 2019 09; 42:190-197.FS

Abstract

The Huaxia™ Platinum Kit for short tandem repeat (STR) amplification was designed to meet the needs of the rapidly growing Chinese forensic database. This PCR multiplex allows simultaneous amplification of the following autosomal loci: D3S1358, vWA, D16S539, CSF1PO, TPOX, D8S1179, D21S11, D18S51, Penta E, D2S441, D19S433, TH01, FGA, D22S1045, D5S818, D13S317, D7S820, D6S1043, D10S1248, D1S1656, D12S391, D2S1338, Penta D and the gender-identification markers Yindel, and AMEL. The Huaxia™ Platinum Kit enables direct amplification from blood and buccal samples stored on treated and untreated paper, and features an optimized PCR protocol that yields time to results in less than 45 min. Developmental validation testing followed SWGDAM guidelines and demonstrated that this assay produces reproducible and accurate results. Studies on 798 individuals in 4 major Chinese ethnic groups produced highly concordant results with other commercially available STR genotyping kits. The validation results demonstrate that the Huaxia™ Platinum Kit is a robust and reliable identification system for forensic DNA databasing applications.

Authors+Show Affiliations

Thermo Fisher Scientific Inc., 180 Oyster Point Blvd., South San Francisco, CA 94080, USA. Electronic address: chang.zhong@thermofisher.com.Thermo Fisher Scientific Inc., 180 Oyster Point Blvd., South San Francisco, CA 94080, USA.Thermo Fisher Scientific Inc., 180 Oyster Point Blvd., South San Francisco, CA 94080, USA.Thermo Fisher Scientific Inc., 180 Oyster Point Blvd., South San Francisco, CA 94080, USA.Thermo Fisher Scientific Inc., 180 Oyster Point Blvd., South San Francisco, CA 94080, USA.Thermo Fisher Scientific Inc., 180 Oyster Point Blvd., South San Francisco, CA 94080, USA.Thermo Fisher Scientific Inc., 180 Oyster Point Blvd., South San Francisco, CA 94080, USA.Thermo Fisher Scientific Inc., 180 Oyster Point Blvd., South San Francisco, CA 94080, USA.

Pub Type(s)

Journal Article
Validation Study

Language

eng

PubMed ID

31374458

Citation

Zhong, Chang, et al. "Developmental Validation of the Huaxia™ Platinum PCR Amplification Kit: a 6-dye Multiplex Direct Amplification Assay Designed for Chinese Reference Samples." Forensic Science International. Genetics, vol. 42, 2019, pp. 190-197.
Zhong C, Gopinath S, Norona W, et al. Developmental validation of the Huaxia™ Platinum PCR amplification kit: A 6-dye multiplex direct amplification assay designed for Chinese reference samples. Forensic Sci Int Genet. 2019;42:190-197.
Zhong, C., Gopinath, S., Norona, W., Ge, J., Lagacé, R. E., Wang, D. Y., Short, M. L., & Mulero, J. J. (2019). Developmental validation of the Huaxia™ Platinum PCR amplification kit: A 6-dye multiplex direct amplification assay designed for Chinese reference samples. Forensic Science International. Genetics, 42, 190-197. https://doi.org/10.1016/j.fsigen.2019.07.001
Zhong C, et al. Developmental Validation of the Huaxia™ Platinum PCR Amplification Kit: a 6-dye Multiplex Direct Amplification Assay Designed for Chinese Reference Samples. Forensic Sci Int Genet. 2019;42:190-197. PubMed PMID: 31374458.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Developmental validation of the Huaxia™ Platinum PCR amplification kit: A 6-dye multiplex direct amplification assay designed for Chinese reference samples. AU - Zhong,Chang, AU - Gopinath,Siddhita, AU - Norona,Wilma, AU - Ge,Jianye, AU - Lagacé,Robert E, AU - Wang,Dennis Y, AU - Short,Marc L, AU - Mulero,Julio J, Y1 - 2019/07/25/ PY - 2018/09/26/received PY - 2019/06/21/revised PY - 2019/07/01/accepted PY - 2019/8/3/pubmed PY - 2019/12/20/medline PY - 2019/8/3/entrez KW - Direct amplification KW - Forensic validation studies KW - Huaxia™ Platinum Kit KW - Rapid identification KW - STR SP - 190 EP - 197 JF - Forensic science international. Genetics JO - Forensic Sci Int Genet VL - 42 N2 - The Huaxia™ Platinum Kit for short tandem repeat (STR) amplification was designed to meet the needs of the rapidly growing Chinese forensic database. This PCR multiplex allows simultaneous amplification of the following autosomal loci: D3S1358, vWA, D16S539, CSF1PO, TPOX, D8S1179, D21S11, D18S51, Penta E, D2S441, D19S433, TH01, FGA, D22S1045, D5S818, D13S317, D7S820, D6S1043, D10S1248, D1S1656, D12S391, D2S1338, Penta D and the gender-identification markers Yindel, and AMEL. The Huaxia™ Platinum Kit enables direct amplification from blood and buccal samples stored on treated and untreated paper, and features an optimized PCR protocol that yields time to results in less than 45 min. Developmental validation testing followed SWGDAM guidelines and demonstrated that this assay produces reproducible and accurate results. Studies on 798 individuals in 4 major Chinese ethnic groups produced highly concordant results with other commercially available STR genotyping kits. The validation results demonstrate that the Huaxia™ Platinum Kit is a robust and reliable identification system for forensic DNA databasing applications. SN - 1878-0326 UR - https://www.unboundmedicine.com/medline/citation/31374458/Developmental_validation_of_the_Huaxia™_Platinum_PCR_amplification_kit:_A_6_dye_multiplex_direct_amplification_assay_designed_for_Chinese_reference_samples_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1872-4973(18)30520-9 DB - PRIME DP - Unbound Medicine ER -