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Nigella sativa stimulates insulin secretion from isolated rat islets and inhibits the digestion and absorption of (CH2O)n in the gut.
Biosci Rep 2019; 39(8)BR

Abstract

Nigella sativa seeds are traditionally reputed as possessing anti-diabetic properties. As a result, we aim to explore the mechanism of its anti-hyperglycemic activity. The present study uses various experimental designs including gastrointestinal (GI) motility, intestinal disaccharidase activity and inhibition of carbohydrate digestion and absorption in the gut. The animals used as type 2 diabetic models were induced with streptozotocin to make them as such. Oral glucose tolerance test was performed to confirm that the animals were indeed diabetic. The extract reduced postprandial glucose, suggesting it interfered with glucose absorption in the gut. It also improved glucose (2.5g/kg, b/w) tolerance in rats. Furthermore, treatment with N. sativa produced a significant improvement in GI motility, while reduced disaccharidase enzyme activity in fasted rats. The extract produced a similar effect within an acute oral sucrose (2.5g/kg, b/w) load assay. Following sucrose administration, a substantial amount of unabsorbed sucrose was found in six different parts of the GI tract. This indicates that N. sativa has the potentiality to liberate GI content and reduce or delay glucose absorption. A potential hypoglycemic activity of the extract found in insulin release assay, where the extract significantly improved insulin secretion from isolated rat islets. These concluded present findings give rise to the implication that N. sativa seeds are generating postprandial anti-hyperglycemic activity within type 2 diabetic animal models via reducing or delaying carbohydrate digestion and absorption in the gut as well as improving insulin secretion in response to the plasma glucose.

Authors+Show Affiliations

Department of Pharmacy, Independent University, Bangladesh (IUB) Bashundhara R/A, Dhaka1229, Bangladesh jmahannan@iub.edu.bd.School of Biomedical Sciences, Ulster University, Coleraine BT52 1SA, Co. Londonderry, Northern Ireland, United Kingdom.Department of Pharmacy, East West University, Dhaka-1212, Bangladesh.Department of Pharmacy, East West University, Dhaka-1212, Bangladesh.Department of Pharmacy, East West University, Dhaka-1212, Bangladesh.Department of Pharmacy, East West University, Dhaka-1212, Bangladesh.Department of Pharmacy, East West University, Dhaka-1212, Bangladesh.Department of Integrated Bioscience, Graduate School, Konkuk University, Chungju- 7478, Republic of Korea.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31375555

Citation

Hannan, J M A., et al. "Nigella Sativa Stimulates Insulin Secretion From Isolated Rat Islets and Inhibits the Digestion and Absorption of (CH2O)n in the Gut." Bioscience Reports, vol. 39, no. 8, 2019.
Hannan JMA, Ansari P, Haque A, et al. Nigella sativa stimulates insulin secretion from isolated rat islets and inhibits the digestion and absorption of (CH2O)n in the gut. Biosci Rep. 2019;39(8).
Hannan, J. M. A., Ansari, P., Haque, A., Sanju, A., Huzaifa, A., Rahman, A., ... Azam, S. (2019). Nigella sativa stimulates insulin secretion from isolated rat islets and inhibits the digestion and absorption of (CH2O)n in the gut. Bioscience Reports, 39(8), doi:10.1042/BSR20190723.
Hannan JMA, et al. Nigella Sativa Stimulates Insulin Secretion From Isolated Rat Islets and Inhibits the Digestion and Absorption of (CH2O)n in the Gut. Biosci Rep. 2019 Aug 30;39(8) PubMed PMID: 31375555.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nigella sativa stimulates insulin secretion from isolated rat islets and inhibits the digestion and absorption of (CH2O)n in the gut. AU - Hannan,J M A, AU - Ansari,Prawej, AU - Haque,Afra, AU - Sanju,Afrina, AU - Huzaifa,Abir, AU - Rahman,Anisur, AU - Ghosh,Adity, AU - Azam,Shofiul, Y1 - 2019/08/23/ PY - 2019/03/23/received PY - 2019/07/25/revised PY - 2019/08/01/accepted PY - 2019/8/4/pubmed PY - 2019/8/4/medline PY - 2019/8/4/entrez KW - Disaccharidase enzyme activity KW - GI motility KW - Glucose tolerance KW - Gut perfusion KW - Nigella sativa KW - Sucrose malabsorption JF - Bioscience reports JO - Biosci. Rep. VL - 39 IS - 8 N2 - Nigella sativa seeds are traditionally reputed as possessing anti-diabetic properties. As a result, we aim to explore the mechanism of its anti-hyperglycemic activity. The present study uses various experimental designs including gastrointestinal (GI) motility, intestinal disaccharidase activity and inhibition of carbohydrate digestion and absorption in the gut. The animals used as type 2 diabetic models were induced with streptozotocin to make them as such. Oral glucose tolerance test was performed to confirm that the animals were indeed diabetic. The extract reduced postprandial glucose, suggesting it interfered with glucose absorption in the gut. It also improved glucose (2.5g/kg, b/w) tolerance in rats. Furthermore, treatment with N. sativa produced a significant improvement in GI motility, while reduced disaccharidase enzyme activity in fasted rats. The extract produced a similar effect within an acute oral sucrose (2.5g/kg, b/w) load assay. Following sucrose administration, a substantial amount of unabsorbed sucrose was found in six different parts of the GI tract. This indicates that N. sativa has the potentiality to liberate GI content and reduce or delay glucose absorption. A potential hypoglycemic activity of the extract found in insulin release assay, where the extract significantly improved insulin secretion from isolated rat islets. These concluded present findings give rise to the implication that N. sativa seeds are generating postprandial anti-hyperglycemic activity within type 2 diabetic animal models via reducing or delaying carbohydrate digestion and absorption in the gut as well as improving insulin secretion in response to the plasma glucose. SN - 1573-4935 UR - https://www.unboundmedicine.com/medline/citation/31375555/Nigella_sativa_stimulates_insulin_secretion_from_isolated_rat_islets_and_inhibits_the_digestion_and_absorption_of_(CH2O)n_in_the_gut L2 - https://portlandpress.com/bioscirep/article-lookup/doi/10.1042/BSR20190723 DB - PRIME DP - Unbound Medicine ER -