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Miniaturized microscope with flexible light source input for neuronal imaging and manipulation in freely behaving animals.
Biochem Biophys Res Commun 2019; 517(3):520-524BB

Abstract

Simultaneous imaging and manipulation of a genetically defined neuronal population can provide a causal link between its activity and function. Here, we designed a miniaturized microscope (or 'miniscope') that allows fluorescence imaging and optogenetic manipulation at the cellular level in freely behaving animals. This miniscope has an integrated optical connector that accepts any combination of external light sources, allowing flexibility in the choice of sensors and manipulators. Moreover, due to its simple structure and use of open source software, the miniscope is easy to build and modify. Using this miniscope, we demonstrate the optogenetic silencing of hippocampal CA1 neurons using two laser light sources-one stimulating a calcium sensor (i.e., jGCaAMP7c) and the other serving as an optogenetic silencer (i.e., Jaws). This new miniscope can contribute to efforts to determine causal relationships between neuronal network dynamics and animal behavior.

Authors+Show Affiliations

International Institute for Integrative Sleep Medicine (WPI-IIIS), University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-0006, Japan.International Institute for Integrative Sleep Medicine (WPI-IIIS), University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-0006, Japan.International Institute for Integrative Sleep Medicine (WPI-IIIS), University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-0006, Japan.International Institute for Integrative Sleep Medicine (WPI-IIIS), University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-0006, Japan.International Institute for Integrative Sleep Medicine (WPI-IIIS), University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-0006, Japan.International Institute for Integrative Sleep Medicine (WPI-IIIS), University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-0006, Japan.International Institute for Integrative Sleep Medicine (WPI-IIIS), University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-0006, Japan. Electronic address: masanori.sakaguchi@gmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31376934

Citation

Srinivasan, Sakthivel, et al. "Miniaturized Microscope With Flexible Light Source Input for Neuronal Imaging and Manipulation in Freely Behaving Animals." Biochemical and Biophysical Research Communications, vol. 517, no. 3, 2019, pp. 520-524.
Srinivasan S, Hosokawa T, Vergara P, et al. Miniaturized microscope with flexible light source input for neuronal imaging and manipulation in freely behaving animals. Biochem Biophys Res Commun. 2019;517(3):520-524.
Srinivasan, S., Hosokawa, T., Vergara, P., Chérasse, Y., Naoi, T., Sakurai, T., & Sakaguchi, M. (2019). Miniaturized microscope with flexible light source input for neuronal imaging and manipulation in freely behaving animals. Biochemical and Biophysical Research Communications, 517(3), pp. 520-524. doi:10.1016/j.bbrc.2019.07.082.
Srinivasan S, et al. Miniaturized Microscope With Flexible Light Source Input for Neuronal Imaging and Manipulation in Freely Behaving Animals. Biochem Biophys Res Commun. 2019 Sep 24;517(3):520-524. PubMed PMID: 31376934.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Miniaturized microscope with flexible light source input for neuronal imaging and manipulation in freely behaving animals. AU - Srinivasan,Sakthivel, AU - Hosokawa,Takuma, AU - Vergara,Pablo, AU - Chérasse,Yoan, AU - Naoi,Toshie, AU - Sakurai,Takeshi, AU - Sakaguchi,Masanori, Y1 - 2019/07/31/ PY - 2019/07/19/received PY - 2019/07/22/accepted PY - 2019/8/5/pubmed PY - 2019/8/5/medline PY - 2019/8/5/entrez KW - GCaMP KW - Hippocampus KW - Miniaturized microscope KW - Optogenetics KW - UCLA miniscope SP - 520 EP - 524 JF - Biochemical and biophysical research communications JO - Biochem. Biophys. Res. Commun. VL - 517 IS - 3 N2 - Simultaneous imaging and manipulation of a genetically defined neuronal population can provide a causal link between its activity and function. Here, we designed a miniaturized microscope (or 'miniscope') that allows fluorescence imaging and optogenetic manipulation at the cellular level in freely behaving animals. This miniscope has an integrated optical connector that accepts any combination of external light sources, allowing flexibility in the choice of sensors and manipulators. Moreover, due to its simple structure and use of open source software, the miniscope is easy to build and modify. Using this miniscope, we demonstrate the optogenetic silencing of hippocampal CA1 neurons using two laser light sources-one stimulating a calcium sensor (i.e., jGCaAMP7c) and the other serving as an optogenetic silencer (i.e., Jaws). This new miniscope can contribute to efforts to determine causal relationships between neuronal network dynamics and animal behavior. SN - 1090-2104 UR - https://www.unboundmedicine.com/medline/citation/31376934/Miniaturized_microscope_with_flexible_light_source_input_for_neuronal_imaging_and_manipulation_in_freely_behaving_animals L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(19)31436-6 DB - PRIME DP - Unbound Medicine ER -