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Nigella sativa seed extract applicability in preparations against methicillin-resistant Staphylococcus aureus and effects on human dermal fibroblasts viability.
J Ethnopharmacol. 2019 Nov 15; 244:112135.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Nigella sativa L. seed extracts and oils have been embraced by traditional medicine of cultures inhabiting Middle East and North Africa for centuries. Among other uses, it has been applied against dermatitis and eczema often worsened by staphylococcal colonization of the skin.

AIM OF THE STUDY

The study was conducted to evaluate applicability of N. sativa seed extract in antibacterial skin formulations by examination of its activity against methicillin-resistant Staphylococcus aureus as well as cytotoxicity against human dermal fibroblasts.

MATERIALS AND METHODS

Two variants of N. sativa seed extract containing 9.91 and 2.10 % of thymoquinone were prepared by supercritical carbon dioxide extraction. The extracts and standards of their major volatile ingredients; thymoquinone, thymol, p-cymene alongside with the reference antiseptics; chlorquinaldol and a combination of amylmetacresol with 2,4-dichlorobenzyl alcohol were subjected to evaluation of antibacterial efficacy against a collection of Staphylococcus aureus strains. The preparation based on Vaseline containing 1% of N. sativa extract was applied on Mueller-Hinton agar plates and its ability to inhibit S. aureus growth was examined. The MTT assay was employed to study cytotoxic effects of the thymoquinone-rich N. sativa seed extract against HDFa fibroblasts.

RESULTS

N. sativa seed extract and thymoquinone have shown potent bacteriostatic and bactericidal effect against Staphylococcus aureus, including methicillin-resistant strains (MRSA) isolated in Poland. Results suggest that N. sativa seed extract activity against S. aureus should mainly be attributed to thymoquinone, which was effective in concentrations of 4-16 μg/ml. Regarding the activity against S. aureus, thymoquinone was more efficient than a combination of amylmetacresol with 2,4-dichlorobenzyl alcohol and comparable to chlorquinaldol. The Vaseline-based preparation containing N. sativa extract caused growth inhibition comparable to an equally concentrated DMSO solution of the extract. The IC50 of N. sativa extract against HDFa fibroblast was determined at 0.2 mg/ml, which was 2-fold higher than the average MIC and MBC of the extract against S. aureus.

CONCLUSIONS

The observed effectiveness of N. sativa seed extracts against bacteria was found to be dominantly dependent on concentration of thymoquinone. Its efficiency against S. aureus isolates as well as results of cytotoxicity examination against human dermal fibroblasts indicate on its applicability as an antibacterial agent for topical use and motivates further research in this area.

Authors+Show Affiliations

Department of Molecular and Cellular Biology, Intercollegiate Faculty of Biotechnology of University of Gdansk and Medical University of Gdansk, Abrahama Str. 58, 80-307, Gdansk, Poland.Biovico Sp. z o.o, Hutnicza Str. 15 B, 81-061, Gdynia, Poland.Biovico Sp. z o.o, Hutnicza Str. 15 B, 81-061, Gdynia, Poland.Biovico Sp. z o.o, Hutnicza Str. 15 B, 81-061, Gdynia, Poland.Department of Molecular and Cellular Biology, Intercollegiate Faculty of Biotechnology of University of Gdansk and Medical University of Gdansk, Abrahama Str. 58, 80-307, Gdansk, Poland. Electronic address: leszek.kadzinski@biotech.ug.edu.pl.Department of Molecular and Cellular Biology, Intercollegiate Faculty of Biotechnology of University of Gdansk and Medical University of Gdansk, Abrahama Str. 58, 80-307, Gdansk, Poland.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31377263

Citation

Gawron, Grzegorz, et al. "Nigella Sativa Seed Extract Applicability in Preparations Against Methicillin-resistant Staphylococcus Aureus and Effects On Human Dermal Fibroblasts Viability." Journal of Ethnopharmacology, vol. 244, 2019, p. 112135.
Gawron G, Krzyczkowski W, Lemke K, et al. Nigella sativa seed extract applicability in preparations against methicillin-resistant Staphylococcus aureus and effects on human dermal fibroblasts viability. J Ethnopharmacol. 2019;244:112135.
Gawron, G., Krzyczkowski, W., Lemke, K., Ołdak, A., Kadziński, L., & Banecki, B. (2019). Nigella sativa seed extract applicability in preparations against methicillin-resistant Staphylococcus aureus and effects on human dermal fibroblasts viability. Journal of Ethnopharmacology, 244, 112135. https://doi.org/10.1016/j.jep.2019.112135
Gawron G, et al. Nigella Sativa Seed Extract Applicability in Preparations Against Methicillin-resistant Staphylococcus Aureus and Effects On Human Dermal Fibroblasts Viability. J Ethnopharmacol. 2019 Nov 15;244:112135. PubMed PMID: 31377263.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nigella sativa seed extract applicability in preparations against methicillin-resistant Staphylococcus aureus and effects on human dermal fibroblasts viability. AU - Gawron,Grzegorz, AU - Krzyczkowski,Wojciech, AU - Lemke,Krzysztof, AU - Ołdak,Alicja, AU - Kadziński,Leszek, AU - Banecki,Bogdan, Y1 - 2019/08/01/ PY - 2019/05/20/received PY - 2019/07/31/revised PY - 2019/07/31/accepted PY - 2019/8/5/pubmed PY - 2020/5/1/medline PY - 2019/8/5/entrez KW - Antibacterial KW - Cytotoxicity KW - Methicillin-resistant Staphylococcus aureus KW - Nigella sativa KW - Supercritical fluid extraction KW - Thymoquinone SP - 112135 EP - 112135 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 244 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Nigella sativa L. seed extracts and oils have been embraced by traditional medicine of cultures inhabiting Middle East and North Africa for centuries. Among other uses, it has been applied against dermatitis and eczema often worsened by staphylococcal colonization of the skin. AIM OF THE STUDY: The study was conducted to evaluate applicability of N. sativa seed extract in antibacterial skin formulations by examination of its activity against methicillin-resistant Staphylococcus aureus as well as cytotoxicity against human dermal fibroblasts. MATERIALS AND METHODS: Two variants of N. sativa seed extract containing 9.91 and 2.10 % of thymoquinone were prepared by supercritical carbon dioxide extraction. The extracts and standards of their major volatile ingredients; thymoquinone, thymol, p-cymene alongside with the reference antiseptics; chlorquinaldol and a combination of amylmetacresol with 2,4-dichlorobenzyl alcohol were subjected to evaluation of antibacterial efficacy against a collection of Staphylococcus aureus strains. The preparation based on Vaseline containing 1% of N. sativa extract was applied on Mueller-Hinton agar plates and its ability to inhibit S. aureus growth was examined. The MTT assay was employed to study cytotoxic effects of the thymoquinone-rich N. sativa seed extract against HDFa fibroblasts. RESULTS: N. sativa seed extract and thymoquinone have shown potent bacteriostatic and bactericidal effect against Staphylococcus aureus, including methicillin-resistant strains (MRSA) isolated in Poland. Results suggest that N. sativa seed extract activity against S. aureus should mainly be attributed to thymoquinone, which was effective in concentrations of 4-16 μg/ml. Regarding the activity against S. aureus, thymoquinone was more efficient than a combination of amylmetacresol with 2,4-dichlorobenzyl alcohol and comparable to chlorquinaldol. The Vaseline-based preparation containing N. sativa extract caused growth inhibition comparable to an equally concentrated DMSO solution of the extract. The IC50 of N. sativa extract against HDFa fibroblast was determined at 0.2 mg/ml, which was 2-fold higher than the average MIC and MBC of the extract against S. aureus. CONCLUSIONS: The observed effectiveness of N. sativa seed extracts against bacteria was found to be dominantly dependent on concentration of thymoquinone. Its efficiency against S. aureus isolates as well as results of cytotoxicity examination against human dermal fibroblasts indicate on its applicability as an antibacterial agent for topical use and motivates further research in this area. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/31377263/Nigella_sativa_seed_extract_applicability_in_preparations_against_methicillin_resistant_Staphylococcus_aureus_and_effects_on_human_dermal_fibroblasts_viability_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(19)31975-0 DB - PRIME DP - Unbound Medicine ER -