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Mechanisms of enhanced bacterial endospore inactivation during sterilization by ohmic heating.
Bioelectrochemistry 2019; 130:107338B

Abstract

During ohmic heating, the electric field may additionally inactivate bacterial endospores. However, the exact mechanism of action is unclear. Thus, a mechanistic study was carried out, investigating the possible target of electric fields inside the spore. Bacillus subtilis spores were heated by conventional and ohmic heating in a capillary system under almost identical thermal conditions. Wild-type (PS533) spores were used, as well as isogenic mutants lacking certain components known for their contribution to spores' heat resistance: small-acid soluble proteins (SASP) protecting DNA (PS578); the coat covering the spore (PS3328); and the spore germination enzyme SleB (FB122(+)). Treatment-dependent release of the spore core's depot of dipicolinic acid (DPA) was further evaluated. Up to 2.4 log10 additional inactivation of PS533 could be achieved by ohmic heating, compared to conventional heating. The difference varied for the mutants, with a decreasing difference indicating a decreased effect of the electric field and vice versa. In particular, mutant spores lacking SASPs showed a behavior more similar to thermal inactivation alone. The combination of heat and electric field was shown to be necessary for enhanced spore inactivation. Thus, it is hypothesized that either the heat treatment makes the spore susceptible to the electric field, or vice versa.

Authors+Show Affiliations

Institute of Food Technology, University of Natural Resources and Life Sciences (BOKU), Vienna, Austria; Department of Food, Agricultural and Biological Engineering, Ohio State University, Columbus, OH, USA. Electronic address: felix.schottroff@boku.ac.at.Department of Food, Agricultural and Biological Engineering, Ohio State University, Columbus, OH, USA.GNT Europa GmbH, Aachen, Germany.Department of Molecular Biology and Biophysics, UCONN Health, Farmington, CT, USA.Department of Food, Agricultural and Biological Engineering, Ohio State University, Columbus, OH, USA.Institute of Food Technology, University of Natural Resources and Life Sciences (BOKU), Vienna, Austria.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31377394

Citation

Schottroff, Felix, et al. "Mechanisms of Enhanced Bacterial Endospore Inactivation During Sterilization By Ohmic Heating." Bioelectrochemistry (Amsterdam, Netherlands), vol. 130, 2019, p. 107338.
Schottroff F, Pyatkovskyy T, Reineke K, et al. Mechanisms of enhanced bacterial endospore inactivation during sterilization by ohmic heating. Bioelectrochemistry. 2019;130:107338.
Schottroff, F., Pyatkovskyy, T., Reineke, K., Setlow, P., Sastry, S. K., & Jaeger, H. (2019). Mechanisms of enhanced bacterial endospore inactivation during sterilization by ohmic heating. Bioelectrochemistry (Amsterdam, Netherlands), 130, p. 107338. doi:10.1016/j.bioelechem.2019.107338.
Schottroff F, et al. Mechanisms of Enhanced Bacterial Endospore Inactivation During Sterilization By Ohmic Heating. Bioelectrochemistry. 2019;130:107338. PubMed PMID: 31377394.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mechanisms of enhanced bacterial endospore inactivation during sterilization by ohmic heating. AU - Schottroff,Felix, AU - Pyatkovskyy,Taras, AU - Reineke,Kai, AU - Setlow,Peter, AU - Sastry,Sudhir K, AU - Jaeger,Henry, Y1 - 2019/07/26/ PY - 2019/07/01/received PY - 2019/07/24/revised PY - 2019/07/24/accepted PY - 2019/8/5/pubmed PY - 2019/8/5/medline PY - 2019/8/5/entrez KW - Bacillus subtilis spores KW - Differentiation of effects KW - Electrotechnologies KW - Inactivation mechanism KW - Ohmic heating KW - Sterilisation SP - 107338 EP - 107338 JF - Bioelectrochemistry (Amsterdam, Netherlands) JO - Bioelectrochemistry VL - 130 N2 - During ohmic heating, the electric field may additionally inactivate bacterial endospores. However, the exact mechanism of action is unclear. Thus, a mechanistic study was carried out, investigating the possible target of electric fields inside the spore. Bacillus subtilis spores were heated by conventional and ohmic heating in a capillary system under almost identical thermal conditions. Wild-type (PS533) spores were used, as well as isogenic mutants lacking certain components known for their contribution to spores' heat resistance: small-acid soluble proteins (SASP) protecting DNA (PS578); the coat covering the spore (PS3328); and the spore germination enzyme SleB (FB122(+)). Treatment-dependent release of the spore core's depot of dipicolinic acid (DPA) was further evaluated. Up to 2.4 log10 additional inactivation of PS533 could be achieved by ohmic heating, compared to conventional heating. The difference varied for the mutants, with a decreasing difference indicating a decreased effect of the electric field and vice versa. In particular, mutant spores lacking SASPs showed a behavior more similar to thermal inactivation alone. The combination of heat and electric field was shown to be necessary for enhanced spore inactivation. Thus, it is hypothesized that either the heat treatment makes the spore susceptible to the electric field, or vice versa. SN - 1878-562X UR - https://www.unboundmedicine.com/medline/citation/31377394/Mechanisms_of_enhanced_bacterial_endospore_inactivation_during_sterilization_by_ohmic_heating L2 - https://linkinghub.elsevier.com/retrieve/pii/S1567-5394(19)30422-0 DB - PRIME DP - Unbound Medicine ER -