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Straightforward and rapid determination of acrylamide in coffee beans by means of HS-SPME/GC-MS.
Food Chem. 2019 Dec 15; 301:125264.FC

Abstract

A straightforward and rapid preparation procedure for the extraction of the acrylamide from coffee beans, by means of the volatile silylated derivatives of acrylamide (AA) and headspace solid phase microextraction (HS-SPME) is described. Commercially available SPME fibre coated with polydimethylsiloxane (PDMS) was used. The silylation reactions of the AA were executed with N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA). The deuterium labelled d3-acrylamide was used as an internal standard. The acrylamide level was quantified using gas chromatography coupled with the mass spectrometry (GC-MS) in the selected ion monitoring (SIM) mode. The limit of quantification (LOQ) for measuring acrylamide was 3 µg/kg. The described method demonstrates satisfactory repeatability (RSD = 2.6%), with an intermediate precision (RSD = 9.4%) and recovery (99-105%).

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Publisher Full Text (DOI)

Authors+Show Affiliations

Wawrzyniak R
Faculty of Chemistry, Adam Mickiewicz University in Poznań, ul. Umultowska 89 b, 61-614 Poznań, Poland. Electronic address: rafwawrz@amu.edu.pl.
Jasiewicz B
Faculty of Chemistry, Adam Mickiewicz University in Poznań, ul. Umultowska 89 b, 61-614 Poznań, Poland. Electronic address: beatakoz@amu.edu.pl.

MeSH

AcrylamideCoffeaCoffeeDimethylpolysiloxanesGas Chromatography-Mass SpectrometrySeedsSolid Phase Microextraction

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31377623

Citation

Wawrzyniak, Rafał, and Beata Jasiewicz. "Straightforward and Rapid Determination of Acrylamide in Coffee Beans By Means of HS-SPME/GC-MS." Food Chemistry, vol. 301, 2019, p. 125264.
Wawrzyniak R, Jasiewicz B. Straightforward and rapid determination of acrylamide in coffee beans by means of HS-SPME/GC-MS. Food Chem. 2019;301:125264.
Wawrzyniak, R., & Jasiewicz, B. (2019). Straightforward and rapid determination of acrylamide in coffee beans by means of HS-SPME/GC-MS. Food Chemistry, 301, 125264. https://doi.org/10.1016/j.foodchem.2019.125264
Wawrzyniak R, Jasiewicz B. Straightforward and Rapid Determination of Acrylamide in Coffee Beans By Means of HS-SPME/GC-MS. Food Chem. 2019 Dec 15;301:125264. PubMed PMID: 31377623.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Straightforward and rapid determination of acrylamide in coffee beans by means of HS-SPME/GC-MS. AU - Wawrzyniak,Rafał, AU - Jasiewicz,Beata, Y1 - 2019/07/26/ PY - 2018/10/22/received PY - 2019/06/19/revised PY - 2019/07/26/accepted PY - 2019/8/5/pubmed PY - 2019/11/15/medline PY - 2019/8/5/entrez KW - Acrylamide (PubChem CID: 6579) KW - Acrylamide-d(3) (PubChem CID: 12209671) KW - BSTFA KW - Deuterium acrylamide KW - GC-MS KW - HS-SPME SP - 125264 EP - 125264 JF - Food chemistry JO - Food Chem VL - 301 N2 - A straightforward and rapid preparation procedure for the extraction of the acrylamide from coffee beans, by means of the volatile silylated derivatives of acrylamide (AA) and headspace solid phase microextraction (HS-SPME) is described. Commercially available SPME fibre coated with polydimethylsiloxane (PDMS) was used. The silylation reactions of the AA were executed with N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA). The deuterium labelled d3-acrylamide was used as an internal standard. The acrylamide level was quantified using gas chromatography coupled with the mass spectrometry (GC-MS) in the selected ion monitoring (SIM) mode. The limit of quantification (LOQ) for measuring acrylamide was 3 µg/kg. The described method demonstrates satisfactory repeatability (RSD = 2.6%), with an intermediate precision (RSD = 9.4%) and recovery (99-105%). SN - 1873-7072 UR - https://www.unboundmedicine.com/medline/citation/31377623/Straightforward_and_rapid_determination_of_acrylamide_in_coffee_beans_by_means_of_HS_SPME/GC_MS_ DB - PRIME DP - Unbound Medicine ER -