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Serological reactivity to MPB83 and CFP10/ESAT-6 antigens in three suid hosts of Mycobacterium bovis infection.
Vet Microbiol 2019; 235:285-288VM

Abstract

Domestic pigs and wild suids are susceptible to Mycobacterium bovis (M. bovis) infection and may even serve as reservoir hosts in some situations. Therefore, detection of infected animals is important for understanding their role in the epidemiology of the disease as well as for management and control of bovine tuberculosis. Infected suids develop strong humoral responses, making serological screening a feasible approach to disease surveillance. However, to optimize sensitivity of the antibody assays, it is necessary to identify and incorporate immunodominant antigens recognized by the target species. The objective of this study was to characterize the antigen recognition by three suid species in a commercially available serological test, DPP VetTB Assay. Serum samples from naturally M. bovis-infected domestic pigs, wild boar and common warthogs were tested. MPB83 protein appeared to be the immunodominant antigen recognized by antibodies in all three species. Overall test sensitivity was increased in wild suids when seroreactivity to CFP10/ESAT-6 antigen was included. Infected animals with visible lesions showed more robust antibody responses than those without gross lesions. The high sensitivity and specificity of the DPP VetTB Assay demonstrated in the present study supports the utility of antibody tests employing these antigens in serological screening of the suid species for M. bovis infection.

Authors+Show Affiliations

Department of Science and Technology-National Research Foundation Centre of Excellence for Biomedical TB Research, South African Medical Research Council Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, PO Box 241, Cape Town 8000, South Africa. Electronic address: miller@sun.ac.za.SaBio Instituto de Investigación en Recursos Cinegéticos IREC (CSIC-UCLM-JCCM), Ronda de Toledo 12, 13005 Ciudad Real, Spain.Department of Science and Technology-National Research Foundation Centre of Excellence for Biomedical TB Research, South African Medical Research Council Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, PO Box 241, Cape Town 8000, South Africa.SaBio Instituto de Investigación en Recursos Cinegéticos IREC (CSIC-UCLM-JCCM), Ronda de Toledo 12, 13005 Ciudad Real, Spain; Dpto. de Anatomía y Anatomía Patológica Comparadas, Facultad de Veterinaria, Universidad de Córdoba, Agrifood Excellence International Campus (ceiA3), 14071, Córdoba, Spain.Chembio Diagnostic Systems, Inc., 3661 Horseblock Road, Medford, New York 11763, USA.Chembio Diagnostic Systems, Inc., 3661 Horseblock Road, Medford, New York 11763, USA.Chembio Diagnostic Systems, Inc., 3661 Horseblock Road, Medford, New York 11763, USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31383314

Citation

Miller, Michele A., et al. "Serological Reactivity to MPB83 and CFP10/ESAT-6 Antigens in Three Suid Hosts of Mycobacterium Bovis Infection." Veterinary Microbiology, vol. 235, 2019, pp. 285-288.
Miller MA, Gortazar C, Roos EO, et al. Serological reactivity to MPB83 and CFP10/ESAT-6 antigens in three suid hosts of Mycobacterium bovis infection. Vet Microbiol. 2019;235:285-288.
Miller, M. A., Gortazar, C., Roos, E. O., Risalde, M. A., Johnathan-Lee, A., Sridhara, A. A., & Lyashchenko, K. P. (2019). Serological reactivity to MPB83 and CFP10/ESAT-6 antigens in three suid hosts of Mycobacterium bovis infection. Veterinary Microbiology, 235, pp. 285-288. doi:10.1016/j.vetmic.2019.07.018.
Miller MA, et al. Serological Reactivity to MPB83 and CFP10/ESAT-6 Antigens in Three Suid Hosts of Mycobacterium Bovis Infection. Vet Microbiol. 2019;235:285-288. PubMed PMID: 31383314.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Serological reactivity to MPB83 and CFP10/ESAT-6 antigens in three suid hosts of Mycobacterium bovis infection. AU - Miller,Michele A, AU - Gortazar,Christian, AU - Roos,Eduard O, AU - Risalde,Maria A, AU - Johnathan-Lee,Ashley, AU - Sridhara,Archana A, AU - Lyashchenko,Konstantin P, Y1 - 2019/07/22/ PY - 2019/01/11/received PY - 2019/07/10/revised PY - 2019/07/19/accepted PY - 2019/8/7/entrez KW - Domestic pigs KW - Immunodominant antigens KW - Mycobacterium bovis KW - Serology KW - Warthogs KW - Wild boar SP - 285 EP - 288 JF - Veterinary microbiology JO - Vet. Microbiol. VL - 235 N2 - Domestic pigs and wild suids are susceptible to Mycobacterium bovis (M. bovis) infection and may even serve as reservoir hosts in some situations. Therefore, detection of infected animals is important for understanding their role in the epidemiology of the disease as well as for management and control of bovine tuberculosis. Infected suids develop strong humoral responses, making serological screening a feasible approach to disease surveillance. However, to optimize sensitivity of the antibody assays, it is necessary to identify and incorporate immunodominant antigens recognized by the target species. The objective of this study was to characterize the antigen recognition by three suid species in a commercially available serological test, DPP VetTB Assay. Serum samples from naturally M. bovis-infected domestic pigs, wild boar and common warthogs were tested. MPB83 protein appeared to be the immunodominant antigen recognized by antibodies in all three species. Overall test sensitivity was increased in wild suids when seroreactivity to CFP10/ESAT-6 antigen was included. Infected animals with visible lesions showed more robust antibody responses than those without gross lesions. The high sensitivity and specificity of the DPP VetTB Assay demonstrated in the present study supports the utility of antibody tests employing these antigens in serological screening of the suid species for M. bovis infection. SN - 1873-2542 UR - https://www.unboundmedicine.com/medline/citation/31383314/Serological_reactivity_to_MPB83_and_CFP10/ESAT-6_antigens_in_three_suid_hosts_of_Mycobacterium_bovis_infection L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-1135(19)30056-2 DB - PRIME DP - Unbound Medicine ER -