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A simple technique for assessing the cuticular diffusion of humic acid biostimulants.
Plant Methods 2019; 15:83PM

Abstract

Background

Experimental determination of the extent and rate of transport of liquid humates supplied to plants is critical in testing physiological effects of such biostimulants which are often supplied as foliar sprays. Therefore, an original experimental method for the qualitative investigation and quantitative description of the penetration of humates through plant cuticles is proposed, tested, and evaluated.

Results

The proposed method involves the isolation of model plant leaf cuticles and the subsequent in vitro evaluation of cuticular humate transport. The employed novel methodology is based on a simple diffusion couple arrangement involving continuous spectrophotometric determination of the amount of penetrated humate in a hydrogel diffusion medium. Prunus laurocerasus leaf cuticles were isolated by chemical and enzymatic treatment and the rate of cuticular penetration of a commercial humate (lignohumate) was estimated over time in quantitative and qualitative terms. Different rates of lignohumate transport were determined for abaxial and adaxial leaf cuticles also in relation to the different cuticular extraction methods tested.

Conclusions

The proposed methodology represents a simple and cheap experimental tool for the study on the trans-cuticular penetration of humic-based biostimulants.

Authors+Show Affiliations

1Institute of Physical and Applied Chemistry, Brno University of Technology, Faculty of Chemistry, Purkynova 464/118, 612 00 Brno, Czech Republic.2Materials Research Centre, Brno University of Technology, Faculty of Chemistry, Purkynova 464/118, 612 00 Brno, Czech Republic.2Materials Research Centre, Brno University of Technology, Faculty of Chemistry, Purkynova 464/118, 612 00 Brno, Czech Republic.1Institute of Physical and Applied Chemistry, Brno University of Technology, Faculty of Chemistry, Purkynova 464/118, 612 00 Brno, Czech Republic.1Institute of Physical and Applied Chemistry, Brno University of Technology, Faculty of Chemistry, Purkynova 464/118, 612 00 Brno, Czech Republic.1Institute of Physical and Applied Chemistry, Brno University of Technology, Faculty of Chemistry, Purkynova 464/118, 612 00 Brno, Czech Republic.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31384288

Citation

Smilkova, Marcela, et al. "A Simple Technique for Assessing the Cuticular Diffusion of Humic Acid Biostimulants." Plant Methods, vol. 15, 2019, p. 83.
Smilkova M, Smilek J, Kalina M, et al. A simple technique for assessing the cuticular diffusion of humic acid biostimulants. Plant Methods. 2019;15:83.
Smilkova, M., Smilek, J., Kalina, M., Klucakova, M., Pekar, M., & Sedlacek, P. (2019). A simple technique for assessing the cuticular diffusion of humic acid biostimulants. Plant Methods, 15, p. 83. doi:10.1186/s13007-019-0469-x.
Smilkova M, et al. A Simple Technique for Assessing the Cuticular Diffusion of Humic Acid Biostimulants. Plant Methods. 2019;15:83. PubMed PMID: 31384288.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A simple technique for assessing the cuticular diffusion of humic acid biostimulants. AU - Smilkova,Marcela, AU - Smilek,Jiri, AU - Kalina,Michal, AU - Klucakova,Martina, AU - Pekar,Miloslav, AU - Sedlacek,Petr, Y1 - 2019/07/31/ PY - 2019/04/02/received PY - 2019/07/23/accepted PY - 2019/8/7/entrez PY - 2019/8/7/pubmed PY - 2019/8/7/medline KW - Diffusion KW - Humic substances KW - Hydrogel KW - Liquid fertilization KW - Plant cuticle KW - Prunus laurocerasus SP - 83 EP - 83 JF - Plant methods JO - Plant Methods VL - 15 N2 - Background: Experimental determination of the extent and rate of transport of liquid humates supplied to plants is critical in testing physiological effects of such biostimulants which are often supplied as foliar sprays. Therefore, an original experimental method for the qualitative investigation and quantitative description of the penetration of humates through plant cuticles is proposed, tested, and evaluated. Results: The proposed method involves the isolation of model plant leaf cuticles and the subsequent in vitro evaluation of cuticular humate transport. The employed novel methodology is based on a simple diffusion couple arrangement involving continuous spectrophotometric determination of the amount of penetrated humate in a hydrogel diffusion medium. Prunus laurocerasus leaf cuticles were isolated by chemical and enzymatic treatment and the rate of cuticular penetration of a commercial humate (lignohumate) was estimated over time in quantitative and qualitative terms. Different rates of lignohumate transport were determined for abaxial and adaxial leaf cuticles also in relation to the different cuticular extraction methods tested. Conclusions: The proposed methodology represents a simple and cheap experimental tool for the study on the trans-cuticular penetration of humic-based biostimulants. SN - 1746-4811 UR - https://www.unboundmedicine.com/medline/citation/31384288/A_simple_technique_for_assessing_the_cuticular_diffusion_of_humic_acid_biostimulants L2 - https://plantmethods.biomedcentral.com/articles/10.1186/s13007-019-0469-x DB - PRIME DP - Unbound Medicine ER -