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Metabolic impact of red blood cell exchange with rejuvenated red blood cells in sickle cell patients.
Transfusion. 2019 10; 59(10):3102-3112.T

Abstract

BACKGROUND

Red blood cell exchange (RCE) transfusions are a mainstay in the treatment of sickle cell anemia (SCA), and allow a temporary restoration of physiological parameters with respect to erythrocyte oxygen carrying capacity and systems metabolism. Recently, we noted that 1) RCE significantly impacts recipients' metabolism in SCA; 2) fresh and end-of-storage red blood cell (RBC) units differently impact systems of metabolism in healthy autologous recipients; and 3) phosphate/inosine/pyruvate/adenine (PIPA) solution reverses the metabolic age of stored RBCs. Therefore, we hypothesized that RCE with PIPA-treated RBC units could further increase the metabolic benefits of RCE in SCA patients.

STUDY DESIGN AND METHODS

Circulating plasma and erythrocytes were collected from patients with SCA before and after RCE, with either conventional or PIPA-treated RBC units, prior to metabolomics analyses.

RESULTS

Consistent with prior work, RCE significantly decreased circulating levels of markers of systemic hypoxemia (lactate, succinate) and decreased plasma levels of acyl-carnitines and amino acids. However, PIPA-treated exchanges were superior to untreated RCEs, with a higher energy state and an increased capacity to activate the pentose phosphate pathway and glutamine metabolism. In addition, RBCs and plasma from recipients of PIPA-treated RBC units resulted in significantly decreased levels of post-transfusion plasticizers, though at the expense of higher circulating levels of oxidized purines (hypoxanthine, xanthine, and the antioxidant urate).

CONCLUSION

Transfusion of PIPA-treated RBCs further increases the metabolic benefits of RCE to patients with SCA, significantly reducing the levels of post-transfusion plasticizers.

Authors+Show Affiliations

Department of Biochemistry and Molecular Genetics, University of Colorado Denver - Anschutz Medical Campus, Aurora, Colorado.Duke University Medical Center, Durham, North Carolina.Department of Biochemistry and Molecular Genetics, University of Colorado Denver - Anschutz Medical Campus, Aurora, Colorado.Duke University School of Medicine, Durham, North Carolina.Duke University School of Medicine, Durham, North Carolina. Department of Surgery, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania.Zimmer Biomet, Braintree, Massachusetts.Zimmer Biomet, Braintree, Massachusetts.Duke University Medical Center, Durham, North Carolina.Department of Biochemistry and Molecular Genetics, University of Colorado Denver - Anschutz Medical Campus, Aurora, Colorado.

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31385330

Citation

Gehrke, Sarah, et al. "Metabolic Impact of Red Blood Cell Exchange With Rejuvenated Red Blood Cells in Sickle Cell Patients." Transfusion, vol. 59, no. 10, 2019, pp. 3102-3112.
Gehrke S, Shah N, Gamboni F, et al. Metabolic impact of red blood cell exchange with rejuvenated red blood cells in sickle cell patients. Transfusion. 2019;59(10):3102-3112.
Gehrke, S., Shah, N., Gamboni, F., Kamyszek, R., Srinivasan, A. J., Gray, A., Landrigan, M., Welsby, I., & D'Alessandro, A. (2019). Metabolic impact of red blood cell exchange with rejuvenated red blood cells in sickle cell patients. Transfusion, 59(10), 3102-3112. https://doi.org/10.1111/trf.15467
Gehrke S, et al. Metabolic Impact of Red Blood Cell Exchange With Rejuvenated Red Blood Cells in Sickle Cell Patients. Transfusion. 2019;59(10):3102-3112. PubMed PMID: 31385330.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Metabolic impact of red blood cell exchange with rejuvenated red blood cells in sickle cell patients. AU - Gehrke,Sarah, AU - Shah,Nirmish, AU - Gamboni,Fabia, AU - Kamyszek,Reed, AU - Srinivasan,Amudan J, AU - Gray,Alan, AU - Landrigan,Matthew, AU - Welsby,Ian, AU - D'Alessandro,Angelo, Y1 - 2019/08/05/ PY - 2019/05/09/received PY - 2019/07/01/revised PY - 2019/07/02/accepted PY - 2019/8/7/pubmed PY - 2020/5/30/medline PY - 2019/8/7/entrez SP - 3102 EP - 3112 JF - Transfusion JO - Transfusion VL - 59 IS - 10 N2 - BACKGROUND: Red blood cell exchange (RCE) transfusions are a mainstay in the treatment of sickle cell anemia (SCA), and allow a temporary restoration of physiological parameters with respect to erythrocyte oxygen carrying capacity and systems metabolism. Recently, we noted that 1) RCE significantly impacts recipients' metabolism in SCA; 2) fresh and end-of-storage red blood cell (RBC) units differently impact systems of metabolism in healthy autologous recipients; and 3) phosphate/inosine/pyruvate/adenine (PIPA) solution reverses the metabolic age of stored RBCs. Therefore, we hypothesized that RCE with PIPA-treated RBC units could further increase the metabolic benefits of RCE in SCA patients. STUDY DESIGN AND METHODS: Circulating plasma and erythrocytes were collected from patients with SCA before and after RCE, with either conventional or PIPA-treated RBC units, prior to metabolomics analyses. RESULTS: Consistent with prior work, RCE significantly decreased circulating levels of markers of systemic hypoxemia (lactate, succinate) and decreased plasma levels of acyl-carnitines and amino acids. However, PIPA-treated exchanges were superior to untreated RCEs, with a higher energy state and an increased capacity to activate the pentose phosphate pathway and glutamine metabolism. In addition, RBCs and plasma from recipients of PIPA-treated RBC units resulted in significantly decreased levels of post-transfusion plasticizers, though at the expense of higher circulating levels of oxidized purines (hypoxanthine, xanthine, and the antioxidant urate). CONCLUSION: Transfusion of PIPA-treated RBCs further increases the metabolic benefits of RCE to patients with SCA, significantly reducing the levels of post-transfusion plasticizers. SN - 1537-2995 UR - https://www.unboundmedicine.com/medline/citation/31385330/Metabolic_impact_of_red_blood_cell_exchange_with_rejuvenated_red_blood_cells_in_sickle_cell_patients_ L2 - https://doi.org/10.1111/trf.15467 DB - PRIME DP - Unbound Medicine ER -