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Middle East Respiratory Syndrome Coronavirus (MERS-CoV) in Dromedary Camels in Africa and Middle East.
Viruses. 2019 08 05; 11(8)V

Abstract

: Dromedary camels are the natural reservoirs of the Middle East respiratory syndrome coronavirus (MERS-CoV). Camels are mostly bred in East African countries then exported into Africa and Middle East for consumption. To understand the distribution of MERS-CoV among camels in North Africa and the Middle East, we conducted surveillance in Egypt, Senegal, Tunisia, Uganda, Jordan, Saudi Arabia, and Iraq. We also performed longitudinal studies of three camel herds in Egypt and Jordan to elucidate MERS-CoV infection and transmission. Between 2016 and 2018, a total of 4027 nasal swabs and 3267 serum samples were collected from all countries. Real- time PCR revealed that MERS-CoV RNA was detected in nasal swab samples from Egypt, Senegal, Tunisia, and Saudi Arabia. Microneutralization assay showed that antibodies were detected in all countries. Positive PCR samples were partially sequenced, and a phylogenetic tree was built. The tree suggested that all sequences are of clade C and sequences from camels in Egypt formed a separate group from previously published sequences. Longitudinal studies showed high seroprevalence in adult camels. These results indicate the widespread distribution of the virus in camels. A systematic active surveillance and longitudinal studies for MERS-CoV are needed to understand the epidemiology of the disease and dynamics of viral infection.

Authors+Show Affiliations

Center of Scientific Excellence for Influenza Virus, Environmental Research Division, National Research Centre, Giza 12622, Egypt.Center of Scientific Excellence for Influenza Virus, Environmental Research Division, National Research Centre, Giza 12622, Egypt.Center of Scientific Excellence for Influenza Virus, Environmental Research Division, National Research Centre, Giza 12622, Egypt.Center of Scientific Excellence for Influenza Virus, Environmental Research Division, National Research Centre, Giza 12622, Egypt.Center of Scientific Excellence for Influenza Virus, Environmental Research Division, National Research Centre, Giza 12622, Egypt.Center of excellence in Bionanoscience Research, King Abdulaziz University, Jeddah 80203, Saudi Arabia. Biotechnology Research Group, Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah 80203, Saudi Arabia.Nature Link, Sfax 3000, Tunisia.Nature Link, Sfax 3000, Tunisia.Department of Biology, Jordan University of Science and Technology, Irbid 22110, Jordan.Department of Life and Earth Sciences, Faculty of Sciences II, Lebanese University, Al Fanar 90656, Lebanon.Department of Epidemiology and Biostatistics, School of Medicine, Makerere University, Kampala 7062, Uganda.Department of Epidemiology and Biostatistics, School of Medicine, Makerere University, Kampala 7062, Uganda.Department of Epidemiology and Biostatistics, School of Medicine, Makerere University, Kampala 7062, Uganda.Department of Genebank and Genetic Sequence, Forensic DNA Research and Training Center, Al-Nahrain University, Baghdad 10072, Iraq.Department of Biological Sciences, King Abdulaziz University, Jeddah 80203, Saudi Arabia.Department of Biological Sciences, King Abdulaziz University, Jeddah 80203, Saudi Arabia. Princess Doctor Najla Saud Al-Saud Distinguished Research Center for Biotechnology, Jeddah 22252, Saudi Arabia. Department of Genetics, Faculty of Agriculture, Ain Shams University, Cairo 11241, Egypt.King Fahad Medical Center, King Abdulaziz University, Jeddah 80203, Saudi Arabia.Center of Scientific Excellence for Influenza Virus, Environmental Research Division, National Research Centre, Giza 12622, Egypt.Center of Scientific Excellence for Influenza Virus, Environmental Research Division, National Research Centre, Giza 12622, Egypt.Center of Scientific Excellence for Influenza Virus, Environmental Research Division, National Research Centre, Giza 12622, Egypt.Center of Scientific Excellence for Influenza Virus, Environmental Research Division, National Research Centre, Giza 12622, Egypt.Center of Scientific Excellence for Influenza Virus, Environmental Research Division, National Research Centre, Giza 12622, Egypt.Center of Scientific Excellence for Influenza Virus, Environmental Research Division, National Research Centre, Giza 12622, Egypt. St. Jude Children's Research Hospital, 262 Danny Thomas Place, Memphis TN 38105, USA.Center of Scientific Excellence for Influenza Virus, Environmental Research Division, National Research Centre, Giza 12622, Egypt.School of Public Health, The University of Hong Kong, 7 Sassoon Rd, Hong Kong, China.School of Public Health, The University of Hong Kong, 7 Sassoon Rd, Hong Kong, China.General Organizations of Veterinary Services, Ministry of Agriculture and Land Reclamation, Nadi Saed St. 1, Dokki, Giza 12618, Egypt.General Organizations of Veterinary Services, Ministry of Agriculture and Land Reclamation, Nadi Saed St. 1, Dokki, Giza 12618, Egypt.Food and Agriculture Organization of the United Nations, Emergency Center for Transboundary, Animal Diseases, Dokki, Giza ‏12611, Egypt.Food and Agriculture Organization of the United Nations, Emergency Center for Transboundary, Animal Diseases, Dokki, Giza ‏12611, Egypt.Animal Health Services (AGAH), Emergency Centre for Transboundary Animal Diseases (ECTAD), Dokki, Giza 12611, Egypt.St. Jude Children's Research Hospital, 262 Danny Thomas Place, Memphis TN 38105, USA.St. Jude Children's Research Hospital, 262 Danny Thomas Place, Memphis TN 38105, USA.School of Public Health, The University of Hong Kong, 7 Sassoon Rd, Hong Kong, China.Animal Health Services (AGAH), Emergency Centre for Transboundary Animal Diseases (ECTAD), Dokki, Giza 12611, Egypt.Center of Scientific Excellence for Influenza Virus, Environmental Research Division, National Research Centre, Giza 12622, Egypt. mohamedahmedali2004@yahoo.com. Center of excellence in Bionanoscience Research, King Abdulaziz University, Jeddah 80203, Saudi Arabia. mohamedahmedali2004@yahoo.com. Biotechnology Research Group, Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah 80203, Saudi Arabia. mohamedahmedali2004@yahoo.com.Human Link, Hazmieh 1109, Lebanon. ghazi@human-link.org. Department of Epidemiology, Human Genetics, and Environmental Sciences, University of Texas, Houston, TX 77030, USA. ghazi@human-link.org.

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

31387326

Citation

Kandeil, Ahmed, et al. "Middle East Respiratory Syndrome Coronavirus (MERS-CoV) in Dromedary Camels in Africa and Middle East." Viruses, vol. 11, no. 8, 2019.
Kandeil A, Gomaa M, Nageh A, et al. Middle East Respiratory Syndrome Coronavirus (MERS-CoV) in Dromedary Camels in Africa and Middle East. Viruses. 2019;11(8).
Kandeil, A., Gomaa, M., Nageh, A., Shehata, M. M., Kayed, A. E., Sabir, J. S. M., Abiadh, A., Jrijer, J., Amr, Z., Said, M. A., Byarugaba, D. K., Wabwire-Mangen, F., Tugume, T., Mohamed, N. S., Attar, R., Hassan, S. M., Linjawi, S. A., Moatassim, Y., Kutkat, O., ... Kayali, G. (2019). Middle East Respiratory Syndrome Coronavirus (MERS-CoV) in Dromedary Camels in Africa and Middle East. Viruses, 11(8). https://doi.org/10.3390/v11080717
Kandeil A, et al. Middle East Respiratory Syndrome Coronavirus (MERS-CoV) in Dromedary Camels in Africa and Middle East. Viruses. 2019 08 5;11(8) PubMed PMID: 31387326.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Middle East Respiratory Syndrome Coronavirus (MERS-CoV) in Dromedary Camels in Africa and Middle East. AU - Kandeil,Ahmed, AU - Gomaa,Mokhtar, AU - Nageh,Ahmed, AU - Shehata,Mahmoud M, AU - Kayed,Ahmed E, AU - Sabir,Jamal S M, AU - Abiadh,Awatef, AU - Jrijer,Jamel, AU - Amr,Zuhair, AU - Said,Mounir Abi, AU - Byarugaba,Denis K, AU - Wabwire-Mangen,Fred, AU - Tugume,Titus, AU - Mohamed,Nadira S, AU - Attar,Roba, AU - Hassan,Sabah M, AU - Linjawi,Sabah Abdulaziz, AU - Moatassim,Yassmin, AU - Kutkat,Omnia, AU - Mahmoud,Sara, AU - Bagato,Ola, AU - Shama,Noura M Abo, AU - El-Shesheny,Rabeh, AU - Mostafa,Ahmed, AU - Perera,Ranawaka Apm, AU - Chu,Daniel Kw, AU - Hassan,Nagla, AU - Elsokary,Basma, AU - Saad,Ahmed, AU - Sobhy,Heba, AU - El Masry,Ihab, AU - McKenzie,Pamela P, AU - Webby,Richard J, AU - Peiris,Malik, AU - Makonnen,Yilma J, AU - Ali,Mohamed A, AU - Kayali,Ghazi, Y1 - 2019/08/05/ PY - 2019/07/09/received PY - 2019/08/01/revised PY - 2019/08/01/accepted PY - 2019/8/8/entrez PY - 2019/8/8/pubmed PY - 2020/9/1/medline KW - MERS coronavirus KW - epidemiology KW - surveillance KW - virus infection KW - virus transmission. JF - Viruses JO - Viruses VL - 11 IS - 8 N2 - : Dromedary camels are the natural reservoirs of the Middle East respiratory syndrome coronavirus (MERS-CoV). Camels are mostly bred in East African countries then exported into Africa and Middle East for consumption. To understand the distribution of MERS-CoV among camels in North Africa and the Middle East, we conducted surveillance in Egypt, Senegal, Tunisia, Uganda, Jordan, Saudi Arabia, and Iraq. We also performed longitudinal studies of three camel herds in Egypt and Jordan to elucidate MERS-CoV infection and transmission. Between 2016 and 2018, a total of 4027 nasal swabs and 3267 serum samples were collected from all countries. Real- time PCR revealed that MERS-CoV RNA was detected in nasal swab samples from Egypt, Senegal, Tunisia, and Saudi Arabia. Microneutralization assay showed that antibodies were detected in all countries. Positive PCR samples were partially sequenced, and a phylogenetic tree was built. The tree suggested that all sequences are of clade C and sequences from camels in Egypt formed a separate group from previously published sequences. Longitudinal studies showed high seroprevalence in adult camels. These results indicate the widespread distribution of the virus in camels. A systematic active surveillance and longitudinal studies for MERS-CoV are needed to understand the epidemiology of the disease and dynamics of viral infection. SN - 1999-4915 UR - https://www.unboundmedicine.com/medline/citation/31387326/Middle_East_Respiratory_Syndrome_Coronavirus__MERS_CoV__in_Dromedary_Camels_in_Africa_and_Middle_East_ L2 - https://www.mdpi.com/resolver?pii=v11080717 DB - PRIME DP - Unbound Medicine ER -