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Maternal conjugated linoleic acid alters hepatic lipid metabolism via the AMPK signaling pathway in chick embryos.
Poult Sci. 2019 Aug 08 [Online ahead of print]PS

Abstract

The effects of maternal conjugated linoleic acid (CLA) on embryonic development and hepatic lipid metabolism were investigated in chick embryos. A total of 180 Arbor Acres female broiler breeders (36 wk old) were randomly divided into the following 3 dietary treatment groups: a basic diet (control), a basic diet containing 0.5% CLA (CLA1), and a basic diet containing 1.0% CLA (CLA2). The females were fed for 8 wk, and the eggs from each group were collected and hatched during the last 2 wk. The results showed that the addition of dietary CLA increased the broken egg rate and reduced the fertilization rate and the egg hatchability (P < 0.05). CLA enrichment decreased the polyunsaturated and monounsaturated fatty acids and increased the saturated fatty acids in the yolk sac (P < 0.05). The yolk sac weight, body weight, and body length had a linear decrease with CLA supplementation (P < 0.05). In the developing chick embryo (at E14) and newly hatched chick (D0), the serum triglyceride concentration decreased with maternal CLA supplementation and was accompanied by a reduction in subcutaneous adipose tissue deposition. In addition, maternal CLA supplementation mediated the hepatic lipid metabolism by decreasing the mRNA expression of sterol regulatory element-binding proteins-1c (SREBP-1c), fatty acid synthase and acetyl-CoA carboxylase, and increasing the mRNA expression of adenosine 5'-monophosphate-activated protein kinase α (AMPKα), peroxisome proliferator-activated receptors α (PPARα), liver fatty acid-binding protein, adipose triglyceride lipase and carnitine palmitoyltransferase in embryonic chick livers (P < 0.05). A drop in SREBP-1c protein expression and an increase in the protein expression of p-AMPKα and PPARα were also observed in the liver of chick embryo (P < 0.05). In conclusion, maternal CLA supplementation regulated the fatty acid composition in the yolk sac, and mediated embryonic chick development and hepatic lipometabolism, and these effects may be related to the AMPK pathway. These findings suggest the potential ability of maternal CLA supplementation to reduce fat deposition in chick embryos.

Authors+Show Affiliations

Poultry Institute, Shandong Academy of Agricultural Sciences, Jinan 250023, China. Shandong Provincial Key Laboratory of Poultry Diseases Diagnosis and Immunology, Jinan 250023, China. Poultry Breeding Engineering Technology Center of Shandong Province, Jinan 250023, China.Poultry Institute, Shandong Academy of Agricultural Sciences, Jinan 250023, China. Shandong Provincial Key Laboratory of Poultry Diseases Diagnosis and Immunology, Jinan 250023, China. Poultry Breeding Engineering Technology Center of Shandong Province, Jinan 250023, China.Haiyang Animal Husbandry & Veterinary Station, Yantai 265100, China.Poultry Institute, Shandong Academy of Agricultural Sciences, Jinan 250023, China. Shandong Provincial Key Laboratory of Poultry Diseases Diagnosis and Immunology, Jinan 250023, China. Poultry Breeding Engineering Technology Center of Shandong Province, Jinan 250023, China.Poultry Institute, Shandong Academy of Agricultural Sciences, Jinan 250023, China. Shandong Provincial Key Laboratory of Poultry Diseases Diagnosis and Immunology, Jinan 250023, China. Poultry Breeding Engineering Technology Center of Shandong Province, Jinan 250023, China.Poultry Institute, Shandong Academy of Agricultural Sciences, Jinan 250023, China. Shandong Provincial Key Laboratory of Poultry Diseases Diagnosis and Immunology, Jinan 250023, China. Poultry Breeding Engineering Technology Center of Shandong Province, Jinan 250023, China.Poultry Institute, Shandong Academy of Agricultural Sciences, Jinan 250023, China. Shandong Provincial Key Laboratory of Poultry Diseases Diagnosis and Immunology, Jinan 250023, China. Poultry Breeding Engineering Technology Center of Shandong Province, Jinan 250023, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31393590

Citation

Fu, Chunyan, et al. "Maternal Conjugated Linoleic Acid Alters Hepatic Lipid Metabolism Via the AMPK Signaling Pathway in Chick Embryos." Poultry Science, 2019.
Fu C, Zhang Y, Yao Q, et al. Maternal conjugated linoleic acid alters hepatic lipid metabolism via the AMPK signaling pathway in chick embryos. Poult Sci. 2019.
Fu, C., Zhang, Y., Yao, Q., Wei, X., Shi, T., Yan, P., & Liu, X. (2019). Maternal conjugated linoleic acid alters hepatic lipid metabolism via the AMPK signaling pathway in chick embryos. Poultry Science. https://doi.org/10.3382/ps/pez462
Fu C, et al. Maternal Conjugated Linoleic Acid Alters Hepatic Lipid Metabolism Via the AMPK Signaling Pathway in Chick Embryos. Poult Sci. 2019 Aug 8; PubMed PMID: 31393590.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Maternal conjugated linoleic acid alters hepatic lipid metabolism via the AMPK signaling pathway in chick embryos. AU - Fu,Chunyan, AU - Zhang,Yan, AU - Yao,Qimeng, AU - Wei,Xiangfa, AU - Shi,Tianhong, AU - Yan,Peipei, AU - Liu,Xuelan, Y1 - 2019/08/08/ PY - 2019/05/07/received PY - 2019/07/31/accepted PY - 2019/8/9/entrez PY - 2019/8/9/pubmed PY - 2019/8/9/medline KW - adenosine 5′-monophosphate- activated protein kinase pathways KW - embryonic development KW - fatty acid composition KW - lipid metabolism KW - maternal conjugated linoleic acid JF - Poultry science JO - Poult Sci N2 - The effects of maternal conjugated linoleic acid (CLA) on embryonic development and hepatic lipid metabolism were investigated in chick embryos. A total of 180 Arbor Acres female broiler breeders (36 wk old) were randomly divided into the following 3 dietary treatment groups: a basic diet (control), a basic diet containing 0.5% CLA (CLA1), and a basic diet containing 1.0% CLA (CLA2). The females were fed for 8 wk, and the eggs from each group were collected and hatched during the last 2 wk. The results showed that the addition of dietary CLA increased the broken egg rate and reduced the fertilization rate and the egg hatchability (P < 0.05). CLA enrichment decreased the polyunsaturated and monounsaturated fatty acids and increased the saturated fatty acids in the yolk sac (P < 0.05). The yolk sac weight, body weight, and body length had a linear decrease with CLA supplementation (P < 0.05). In the developing chick embryo (at E14) and newly hatched chick (D0), the serum triglyceride concentration decreased with maternal CLA supplementation and was accompanied by a reduction in subcutaneous adipose tissue deposition. In addition, maternal CLA supplementation mediated the hepatic lipid metabolism by decreasing the mRNA expression of sterol regulatory element-binding proteins-1c (SREBP-1c), fatty acid synthase and acetyl-CoA carboxylase, and increasing the mRNA expression of adenosine 5'-monophosphate-activated protein kinase α (AMPKα), peroxisome proliferator-activated receptors α (PPARα), liver fatty acid-binding protein, adipose triglyceride lipase and carnitine palmitoyltransferase in embryonic chick livers (P < 0.05). A drop in SREBP-1c protein expression and an increase in the protein expression of p-AMPKα and PPARα were also observed in the liver of chick embryo (P < 0.05). In conclusion, maternal CLA supplementation regulated the fatty acid composition in the yolk sac, and mediated embryonic chick development and hepatic lipometabolism, and these effects may be related to the AMPK pathway. These findings suggest the potential ability of maternal CLA supplementation to reduce fat deposition in chick embryos. SN - 1525-3171 UR - https://www.unboundmedicine.com/medline/citation/31393590/Maternal_conjugated_linoleic_acid_alters_hepatic_lipid_metabolism_via_the_AMPK_signaling_pathway_in_chick_embryos_ L2 - https://linkinghub.elsevier.com/retrieve/pii/5545195 DB - PRIME DP - Unbound Medicine ER -
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