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Microbial Inoculum Composition and Pre-weaned Dairy Calf Age Alter the Developing Rumen Microbial Environment.
Front Microbiol 2019; 10:1651FM

Abstract

The objective of this experiment was to determine if dosing pre-weaned calves with enriched ruminal microbiota alters the rumen microbial environment and growth performance. Twenty Holstein bull calves were removed from their dam at birth, fed 3.8 L colostrum within 4 h after birth, and housed individually. Calves were fed pasteurized milk 3×/d from 0 to 7 weeks of age and offered a texturized calf starter ad libitum at 6 days of age. A randomized complete block design with repeated measures and a 2 × 2 factorial arrangement of treatments was used to evaluate responses. Treatments were administered by stomach intubation once per week from 3 to 6 weeks of age and included: 50 mL autoclaved rumen fluid (RF), 50 mL bacterial-enriched RF (BE), 50 mL protozoal-enriched RF (PE); or 50 mL of each BE and PE inoculum. A rumen content composite was collected from 4 rumen fistulated, lactating cows and used to create the inocula. BE inocula were microscopically confirmed to be free of ciliate protozoa before inoculation, while PE contained 2.9 ± 2.2 × 105 protozoa/mL. RF was collected from the calves once per week before 50 mL of the inoculum was administered. Animal performance (e.g., weight gain and dry matter intake) was not altered by inocula type. All calves were microscopically free of rumen ciliates before inoculum administration and calves that did not receive PE remained ciliate-free. Ciliate protozoa were observed in RF from 6, 8, and 6 PE treated calves (n = 10) at weeks 4, 5, and 6, respectively. Ruminal NH3 was lower in PE treated calves (3.3 vs. 6.8 ± 1.0 mM), while ruminal butyrate molar percent was greater in BE treated calves (10.8 vs. 8.3 ± 0.8). Rumen bacterial diversity measures did not differ by treatment at 3-6 weeks. Individual calf bacterial communities from treated calves became temporarily similar to the inocula at 4 weeks of age, but these communities diverged from the inocula at 5 weeks. This study provides new information about two types of rumen-derived inocula and insight into the challenges of directing the rumen microbial environment in the pre-weaned calf.

Authors+Show Affiliations

Department of Animal Sciences, University of Florida, Gainesville, FL, United States.United States Department of Agriculture (USDA)-Agricultural Research Service, Dairy Forage Research Center, Madison, WI, United States.United States Department of Agriculture (USDA)-Agricultural Research Service, Dairy Forage Research Center, Madison, WI, United States.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31396179

Citation

Cersosimo, Laura M., et al. "Microbial Inoculum Composition and Pre-weaned Dairy Calf Age Alter the Developing Rumen Microbial Environment." Frontiers in Microbiology, vol. 10, 2019, p. 1651.
Cersosimo LM, Radloff W, Zanton GI. Microbial Inoculum Composition and Pre-weaned Dairy Calf Age Alter the Developing Rumen Microbial Environment. Front Microbiol. 2019;10:1651.
Cersosimo, L. M., Radloff, W., & Zanton, G. I. (2019). Microbial Inoculum Composition and Pre-weaned Dairy Calf Age Alter the Developing Rumen Microbial Environment. Frontiers in Microbiology, 10, p. 1651. doi:10.3389/fmicb.2019.01651.
Cersosimo LM, Radloff W, Zanton GI. Microbial Inoculum Composition and Pre-weaned Dairy Calf Age Alter the Developing Rumen Microbial Environment. Front Microbiol. 2019;10:1651. PubMed PMID: 31396179.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Microbial Inoculum Composition and Pre-weaned Dairy Calf Age Alter the Developing Rumen Microbial Environment. AU - Cersosimo,Laura M, AU - Radloff,Wendy, AU - Zanton,Geoffrey I, Y1 - 2019/07/23/ PY - 2019/02/08/received PY - 2019/07/03/accepted PY - 2019/8/10/entrez PY - 2019/8/10/pubmed PY - 2019/8/10/medline KW - bacteria KW - ciliates KW - microbiota KW - protozoa KW - ruminant SP - 1651 EP - 1651 JF - Frontiers in microbiology JO - Front Microbiol VL - 10 N2 - The objective of this experiment was to determine if dosing pre-weaned calves with enriched ruminal microbiota alters the rumen microbial environment and growth performance. Twenty Holstein bull calves were removed from their dam at birth, fed 3.8 L colostrum within 4 h after birth, and housed individually. Calves were fed pasteurized milk 3×/d from 0 to 7 weeks of age and offered a texturized calf starter ad libitum at 6 days of age. A randomized complete block design with repeated measures and a 2 × 2 factorial arrangement of treatments was used to evaluate responses. Treatments were administered by stomach intubation once per week from 3 to 6 weeks of age and included: 50 mL autoclaved rumen fluid (RF), 50 mL bacterial-enriched RF (BE), 50 mL protozoal-enriched RF (PE); or 50 mL of each BE and PE inoculum. A rumen content composite was collected from 4 rumen fistulated, lactating cows and used to create the inocula. BE inocula were microscopically confirmed to be free of ciliate protozoa before inoculation, while PE contained 2.9 ± 2.2 × 105 protozoa/mL. RF was collected from the calves once per week before 50 mL of the inoculum was administered. Animal performance (e.g., weight gain and dry matter intake) was not altered by inocula type. All calves were microscopically free of rumen ciliates before inoculum administration and calves that did not receive PE remained ciliate-free. Ciliate protozoa were observed in RF from 6, 8, and 6 PE treated calves (n = 10) at weeks 4, 5, and 6, respectively. Ruminal NH3 was lower in PE treated calves (3.3 vs. 6.8 ± 1.0 mM), while ruminal butyrate molar percent was greater in BE treated calves (10.8 vs. 8.3 ± 0.8). Rumen bacterial diversity measures did not differ by treatment at 3-6 weeks. Individual calf bacterial communities from treated calves became temporarily similar to the inocula at 4 weeks of age, but these communities diverged from the inocula at 5 weeks. This study provides new information about two types of rumen-derived inocula and insight into the challenges of directing the rumen microbial environment in the pre-weaned calf. SN - 1664-302X UR - https://www.unboundmedicine.com/medline/citation/31396179/Microbial_Inoculum_Composition_and_Pre-weaned_Dairy_Calf_Age_Alter_the_Developing_Rumen_Microbial_Environment L2 - https://dx.doi.org/10.3389/fmicb.2019.01651 DB - PRIME DP - Unbound Medicine ER -