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Effects of polyethylene microplastic on the phytotoxicity of di-n-butyl phthalate in lettuce (Lactuca sativa L. var. ramosa Hort).
Chemosphere 2019; 237:124482C

Abstract

The increase in the proportion of microplastics in the environment has intensified the interest in phthalate and microplastic contamination in recent years. In this study, we investigated the response of photosynthetic parameters and the antioxidant system of lettuce to di-n-butyl phthalate (DBP) stress and exposure to various concentrations of microplastic polyethylene (MP) for different durations (14 d and 28 d). Lettuce growth, photosynthetic parameters, and chlorophyll content were reduced significantly after MP- and DBP-only treatments and after the combined (MP + DBP) treatments with both pollutants (P < 0.05), when compared with the control. Our findings indicated that the exposure to MP can inhibit growth, hinder photosynthesis, and interfere with the antioxidant defense system in lettuce. Specifically, compared with the DBP-only treatment group, in all MP + DBP treatment groups, the lettuce growth parameters (dry and fresh weights of the leaves and roots and the number of leaves) decreased (P < 0.05). Moreover, the photosynthetic rate, stomatal conductance, transient transpiration rate, fluorescence parameters, chlorophyll content of leaves, and activity of Rubisco decreased, but the intercellular CO2 concentration increased in all MP + DBP treatment groups. The reduction in photosynthesis was attributed to the limitation of non-porosity and inhibition of the photoelectron flow, and the increase in exogenous MP content aggravated the effect of DBP on photosynthesis in lettuce. Compared with the DBP-only group, in all MP + DBP treatment groups, the content of superoxide radicals and hydrogen peroxide in lettuce leaves and roots increased. Antioxidant levels increased with the increase in MP content, except in the 1.0 mg mL-1 MP treatment after 14 d. Although the antioxidant system exhibited certain protective effects in the latter treatment, the cell membranes were still damaged. The degree of damage to cells decreased with the growth of lettuce, but the damage to root tissue always remained higher than that of the leaves. In conclusion, exposure to exogenous MP exacerbated the damage to lettuce by DBP.

Authors+Show Affiliations

School of Environmental Science and Engineering, Tianjin Polytechnic University, Tianjin, 300387, China; Department of Civil and Environmental Engineering, Shantou University, Shantou, 515063, China.School of Environmental Science and Engineering, Tianjin Polytechnic University, Tianjin, 300387, China.Department of Civil and Environmental Engineering, Shantou University, Shantou, 515063, China; Agro-Environmental Protection Institute, Ministry of Agriculture of China, Tianjin, 300191, China. Electronic address: forestman1218@163.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31398608

Citation

Gao, Minling, et al. "Effects of Polyethylene Microplastic On the Phytotoxicity of Di-n-butyl Phthalate in Lettuce (Lactuca Sativa L. Var. Ramosa Hort)." Chemosphere, vol. 237, 2019, p. 124482.
Gao M, Liu Y, Song Z. Effects of polyethylene microplastic on the phytotoxicity of di-n-butyl phthalate in lettuce (Lactuca sativa L. var. ramosa Hort). Chemosphere. 2019;237:124482.
Gao, M., Liu, Y., & Song, Z. (2019). Effects of polyethylene microplastic on the phytotoxicity of di-n-butyl phthalate in lettuce (Lactuca sativa L. var. ramosa Hort). Chemosphere, 237, p. 124482. doi:10.1016/j.chemosphere.2019.124482.
Gao M, Liu Y, Song Z. Effects of Polyethylene Microplastic On the Phytotoxicity of Di-n-butyl Phthalate in Lettuce (Lactuca Sativa L. Var. Ramosa Hort). Chemosphere. 2019;237:124482. PubMed PMID: 31398608.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of polyethylene microplastic on the phytotoxicity of di-n-butyl phthalate in lettuce (Lactuca sativa L. var. ramosa Hort). AU - Gao,Minling, AU - Liu,Yu, AU - Song,Zhengguo, Y1 - 2019/07/30/ PY - 2019/05/31/received PY - 2019/07/26/revised PY - 2019/07/28/accepted PY - 2019/8/10/pubmed PY - 2019/8/10/medline PY - 2019/8/10/entrez KW - Di-n-butyl phthalate KW - Microplastic polyethylene KW - Oxidative damage KW - Photosynthesis SP - 124482 EP - 124482 JF - Chemosphere JO - Chemosphere VL - 237 N2 - The increase in the proportion of microplastics in the environment has intensified the interest in phthalate and microplastic contamination in recent years. In this study, we investigated the response of photosynthetic parameters and the antioxidant system of lettuce to di-n-butyl phthalate (DBP) stress and exposure to various concentrations of microplastic polyethylene (MP) for different durations (14 d and 28 d). Lettuce growth, photosynthetic parameters, and chlorophyll content were reduced significantly after MP- and DBP-only treatments and after the combined (MP + DBP) treatments with both pollutants (P < 0.05), when compared with the control. Our findings indicated that the exposure to MP can inhibit growth, hinder photosynthesis, and interfere with the antioxidant defense system in lettuce. Specifically, compared with the DBP-only treatment group, in all MP + DBP treatment groups, the lettuce growth parameters (dry and fresh weights of the leaves and roots and the number of leaves) decreased (P < 0.05). Moreover, the photosynthetic rate, stomatal conductance, transient transpiration rate, fluorescence parameters, chlorophyll content of leaves, and activity of Rubisco decreased, but the intercellular CO2 concentration increased in all MP + DBP treatment groups. The reduction in photosynthesis was attributed to the limitation of non-porosity and inhibition of the photoelectron flow, and the increase in exogenous MP content aggravated the effect of DBP on photosynthesis in lettuce. Compared with the DBP-only group, in all MP + DBP treatment groups, the content of superoxide radicals and hydrogen peroxide in lettuce leaves and roots increased. Antioxidant levels increased with the increase in MP content, except in the 1.0 mg mL-1 MP treatment after 14 d. Although the antioxidant system exhibited certain protective effects in the latter treatment, the cell membranes were still damaged. The degree of damage to cells decreased with the growth of lettuce, but the damage to root tissue always remained higher than that of the leaves. In conclusion, exposure to exogenous MP exacerbated the damage to lettuce by DBP. SN - 1879-1298 UR - https://www.unboundmedicine.com/medline/citation/31398608/Effects_of_polyethylene_microplastic_on_the_phytotoxicity_of_di_n_butyl_phthalate_in_lettuce__Lactuca_sativa_L__var__ramosa_Hort__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0045-6535(19)31706-0 DB - PRIME DP - Unbound Medicine ER -