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Therapeutic effect of DNA vaccine encoding the 60-kDa-heat shock protein from Paracoccidoides brasiliensis on experimental paracoccidioidomycosis in mice.
Vaccine 2019; 37(37):5607-5613V

Abstract

Paracoccidioidomycosis (PCM) is a systemic mycosis autochthonous to Latin America and endemic to Brazil, which has the majority of the PCM cases. PCM is acquired through the inhalation of propagules of fungi from genus Paracoccidioides spp. and mainly affects the lungs. We have previously shown that P. brasiliensis-infected mice treated with single-dose of recombinant 60-kDa-heat shock protein from P. brasiliensis (rPbHsp60) had a worsening infection in comparison to animals only infected. In this study, we investigate whether the treatment of infected mice with PB_HSP60 gene cloned into a plasmid (pVAX1-PB_HSP60) would result in efficient immune response and better control of the disease. The harmful impact of single-dose therapy with protein was not seen with plasmid preparations. Most importantly, three doses of pVAX1-PB_HSP60 and protein induced a beneficial effect in experimental PCM with a reduction in fungal load and lung injury when compared with infected mice treated with pVAX1 or PBS. The increase of the cytokines IFN-γ, TNF, and IL-17 and the decrease of IL-10 observed after treatment with three doses of pVAX1-PB_HSP60 appears to be responsible for the control of infection. These results open perspectives of the therapeutic use of Hsp60 in PCM.

Authors+Show Affiliations

Ribeirão Preto Medical School, University of São Paulo, 14049-900 Ribeirão Preto, SP, Brazil.Paulista University, 14024-270 Ribeirão Preto, SP, Brazil.Ribeirão Preto Medical School, University of São Paulo, 14049-900 Ribeirão Preto, SP, Brazil.Ribeirão Preto Medical School, University of São Paulo, 14049-900 Ribeirão Preto, SP, Brazil.Department of Biology, Faculty of Philosophy, Sciences, and Letters at Ribeirão Preto - University of São Paulo, 14040-901 Ribeirão Preto, SP, Brazil. Electronic address: apcastelo@usp.br.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31399276

Citation

Souza, Igor Emiliano L., et al. "Therapeutic Effect of DNA Vaccine Encoding the 60-kDa-heat Shock Protein From Paracoccidoides Brasiliensis On Experimental Paracoccidioidomycosis in Mice." Vaccine, vol. 37, no. 37, 2019, pp. 5607-5613.
Souza IEL, Fernandes FF, Schiavoni MCL, et al. Therapeutic effect of DNA vaccine encoding the 60-kDa-heat shock protein from Paracoccidoides brasiliensis on experimental paracoccidioidomycosis in mice. Vaccine. 2019;37(37):5607-5613.
Souza, I. E. L., Fernandes, F. F., Schiavoni, M. C. L., Silva, C. L., & Panunto-Castelo, A. (2019). Therapeutic effect of DNA vaccine encoding the 60-kDa-heat shock protein from Paracoccidoides brasiliensis on experimental paracoccidioidomycosis in mice. Vaccine, 37(37), pp. 5607-5613. doi:10.1016/j.vaccine.2019.07.090.
Souza IEL, et al. Therapeutic Effect of DNA Vaccine Encoding the 60-kDa-heat Shock Protein From Paracoccidoides Brasiliensis On Experimental Paracoccidioidomycosis in Mice. Vaccine. 2019 Sep 3;37(37):5607-5613. PubMed PMID: 31399276.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Therapeutic effect of DNA vaccine encoding the 60-kDa-heat shock protein from Paracoccidoides brasiliensis on experimental paracoccidioidomycosis in mice. AU - Souza,Igor Emiliano L, AU - Fernandes,Fabrício F, AU - Schiavoni,Maria Cristina L, AU - Silva,Célio L, AU - Panunto-Castelo,Ademilson, Y1 - 2019/08/06/ PY - 2019/01/04/received PY - 2019/07/20/revised PY - 2019/07/26/accepted PY - 2019/8/11/pubmed PY - 2019/8/11/medline PY - 2019/8/11/entrez KW - DNA vaccine KW - Heat-shock protein KW - Paracoccidioides KW - Paracoccidioidomycosis SP - 5607 EP - 5613 JF - Vaccine JO - Vaccine VL - 37 IS - 37 N2 - Paracoccidioidomycosis (PCM) is a systemic mycosis autochthonous to Latin America and endemic to Brazil, which has the majority of the PCM cases. PCM is acquired through the inhalation of propagules of fungi from genus Paracoccidioides spp. and mainly affects the lungs. We have previously shown that P. brasiliensis-infected mice treated with single-dose of recombinant 60-kDa-heat shock protein from P. brasiliensis (rPbHsp60) had a worsening infection in comparison to animals only infected. In this study, we investigate whether the treatment of infected mice with PB_HSP60 gene cloned into a plasmid (pVAX1-PB_HSP60) would result in efficient immune response and better control of the disease. The harmful impact of single-dose therapy with protein was not seen with plasmid preparations. Most importantly, three doses of pVAX1-PB_HSP60 and protein induced a beneficial effect in experimental PCM with a reduction in fungal load and lung injury when compared with infected mice treated with pVAX1 or PBS. The increase of the cytokines IFN-γ, TNF, and IL-17 and the decrease of IL-10 observed after treatment with three doses of pVAX1-PB_HSP60 appears to be responsible for the control of infection. These results open perspectives of the therapeutic use of Hsp60 in PCM. SN - 1873-2518 UR - https://www.unboundmedicine.com/medline/citation/31399276/Therapeutic_effect_of_DNA_vaccine_encoding_the_60-kDa-heat_shock_protein_from_Paracoccidoides_brasiliensis_on_experimental_paracoccidioidomycosis_in_mice L2 - https://linkinghub.elsevier.com/retrieve/pii/S0264-410X(19)31011-4 DB - PRIME DP - Unbound Medicine ER -