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Glochidion zeylanicum leaf extracts exhibit lifespan extending and oxidative stress resistance properties in Caenorhabditis elegans via DAF-16/FoxO and SKN-1/Nrf-2 signaling pathways.
Phytomedicine 2019; 64:153061P

Abstract

BACKGROUND

Glochidion zeylanicum (GZ), a common plant in Thailand and Eastern Asia, is rich in antioxidants. However, the possible anti-aging and oxidative stress resistance properties of GZ leaf extracts (hexane and methanol extracts) have not been reported.

PURPOSE

We aimed to provide the first science-based evidence of the beneficial effects of GZ on anti-aging and oxidative stress resistance in the Caenorhabditis elegans model.

METHODS

The phytochemical composition of the hexane and methanol extracts were analyzed using GLC-MS and LC-MS. Fingerprinting analysis of the extract was performed by RP-HPLC. We determined total phenolics, flavonoids, and antioxidant properties via DPPH and ABTS assays. Oxidative stress resistance, anti-aging and lifespan were studied in C. elegans treated with leaf extracts.

RESULTS

GZ leaf extracts protected the worms against oxidative stress and attenuated ROS accumulation. The expression of stress-response genes, such as SOD-3, and GST-4 were up-regulated, whereas HSP-16.2 was down-regulated after GZ treatment. The oxidative stress resistance properties of GZ possibly involved the DAF-16/FoxO and SKN-1/Nrf-2 transcription factors. GZ leaf extracts improved pharyngeal pumping function and autofluorescent pigment attenuation suggesting anti-aging properties. GZ leaf extracts modulated the lifespan extension in C. elegans.

CONCLUSION

This study reports novel anti-aging and antioxidant activities of GZ leaf extracts, suggesting a novel bioactivity for a medicinally important plant and supplementary drug against oxidative stress.

Authors+Show Affiliations

Graduate Program in Clinical Biochemistry and Molecular Medicine, Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand; Institute of Pharmacy and Molecular Biotechnology, Im Neuenheimer Feld 364, Heidelberg University, Heidelberg 69120, Germany; Age-Related Inflammation and Degeneration Research Unit, Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand.Graduate Program in Clinical Biochemistry and Molecular Medicine, Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand; Institute of Pharmacy and Molecular Biotechnology, Im Neuenheimer Feld 364, Heidelberg University, Heidelberg 69120, Germany; Age-Related Inflammation and Degeneration Research Unit, Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand.Department of Biotechnology, School of Environmental and Chemical Engineering, Dalian Jiaotong University, Dalian 116028, China; Institute of Pharmacy and Molecular Biotechnology, Im Neuenheimer Feld 364, Heidelberg University, Heidelberg 69120, Germany.College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Institute of Pharmacy and Molecular Biotechnology, Im Neuenheimer Feld 364, Heidelberg University, Heidelberg 69120, Germany.Institute of Pharmacy and Molecular Biotechnology, Im Neuenheimer Feld 364, Heidelberg University, Heidelberg 69120, Germany. Electronic address: wink@uni-heidelberg.de.Graduate Program in Clinical Biochemistry and Molecular Medicine, Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand; Age-Related Inflammation and Degeneration Research Unit, Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand. Electronic address: tewin.t@chula.ac.th.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31401497

Citation

Duangjan, Chatrawee, et al. "Glochidion Zeylanicum Leaf Extracts Exhibit Lifespan Extending and Oxidative Stress Resistance Properties in Caenorhabditis Elegans Via DAF-16/FoxO and SKN-1/Nrf-2 Signaling Pathways." Phytomedicine : International Journal of Phytotherapy and Phytopharmacology, vol. 64, 2019, p. 153061.
Duangjan C, Rangsinth P, Gu X, et al. Glochidion zeylanicum leaf extracts exhibit lifespan extending and oxidative stress resistance properties in Caenorhabditis elegans via DAF-16/FoxO and SKN-1/Nrf-2 signaling pathways. Phytomedicine. 2019;64:153061.
Duangjan, C., Rangsinth, P., Gu, X., Zhang, S., Wink, M., & Tencomnao, T. (2019). Glochidion zeylanicum leaf extracts exhibit lifespan extending and oxidative stress resistance properties in Caenorhabditis elegans via DAF-16/FoxO and SKN-1/Nrf-2 signaling pathways. Phytomedicine : International Journal of Phytotherapy and Phytopharmacology, 64, p. 153061. doi:10.1016/j.phymed.2019.153061.
Duangjan C, et al. Glochidion Zeylanicum Leaf Extracts Exhibit Lifespan Extending and Oxidative Stress Resistance Properties in Caenorhabditis Elegans Via DAF-16/FoxO and SKN-1/Nrf-2 Signaling Pathways. Phytomedicine. 2019 Jul 31;64:153061. PubMed PMID: 31401497.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Glochidion zeylanicum leaf extracts exhibit lifespan extending and oxidative stress resistance properties in Caenorhabditis elegans via DAF-16/FoxO and SKN-1/Nrf-2 signaling pathways. AU - Duangjan,Chatrawee, AU - Rangsinth,Panthakarn, AU - Gu,Xiaojie, AU - Zhang,Shaoxiong, AU - Wink,Michael, AU - Tencomnao,Tewin, Y1 - 2019/07/31/ PY - 2019/05/31/received PY - 2019/07/23/revised PY - 2019/07/29/accepted PY - 2019/8/12/pubmed PY - 2019/8/12/medline PY - 2019/8/12/entrez KW - Aging KW - Antioxidants KW - Caenorhabditis elegans KW - DAF-16/FoxO KW - Glochidion zeylanicum KW - SKN-1/Nrf2 SP - 153061 EP - 153061 JF - Phytomedicine : international journal of phytotherapy and phytopharmacology JO - Phytomedicine VL - 64 N2 - BACKGROUND: Glochidion zeylanicum (GZ), a common plant in Thailand and Eastern Asia, is rich in antioxidants. However, the possible anti-aging and oxidative stress resistance properties of GZ leaf extracts (hexane and methanol extracts) have not been reported. PURPOSE: We aimed to provide the first science-based evidence of the beneficial effects of GZ on anti-aging and oxidative stress resistance in the Caenorhabditis elegans model. METHODS: The phytochemical composition of the hexane and methanol extracts were analyzed using GLC-MS and LC-MS. Fingerprinting analysis of the extract was performed by RP-HPLC. We determined total phenolics, flavonoids, and antioxidant properties via DPPH and ABTS assays. Oxidative stress resistance, anti-aging and lifespan were studied in C. elegans treated with leaf extracts. RESULTS: GZ leaf extracts protected the worms against oxidative stress and attenuated ROS accumulation. The expression of stress-response genes, such as SOD-3, and GST-4 were up-regulated, whereas HSP-16.2 was down-regulated after GZ treatment. The oxidative stress resistance properties of GZ possibly involved the DAF-16/FoxO and SKN-1/Nrf-2 transcription factors. GZ leaf extracts improved pharyngeal pumping function and autofluorescent pigment attenuation suggesting anti-aging properties. GZ leaf extracts modulated the lifespan extension in C. elegans. CONCLUSION: This study reports novel anti-aging and antioxidant activities of GZ leaf extracts, suggesting a novel bioactivity for a medicinally important plant and supplementary drug against oxidative stress. SN - 1618-095X UR - https://www.unboundmedicine.com/medline/citation/31401497/Glochidion_zeylanicum_leaf_extracts_exhibit_lifespan_extending_and_oxidative_stress_resistance_properties_in_Caenorhabditis_elegans_via_DAF_16/FoxO_and_SKN_1/Nrf_2_signaling_pathways_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0944-7113(19)30227-2 DB - PRIME DP - Unbound Medicine ER -