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Nephroprotective Effect of Moringa Oleifera Seed Oil on Gentamicin-Induced Nephrotoxicity in Rats: Biochemical Evaluation of Antioxidant, Anti-inflammatory, and Antiapoptotic Pathways.
J Am Coll Nutr. 2020 May-Jun; 39(4):307-315.JA

Abstract

Objective: Gentamicin is an efficacious aminoglycoside antibiotic widely used to treat life-threatening Gram-negative bacteria infections. However, its specific non-targeted induction of nephrotoxicity is a worrying clinical challenge. The study explored the nephroprotective effect of Moringa oleifera seed oil (MOO) against gentamicin-induced oxidative nephrotoxicity, pro-inflammation, and apoptosis in male Wistar rats.Method: Twenty-four rats divided into 4 groups (n = 6) were administered MOO (5 ml/kg) for 16 days and/or gentamicin (100 mg/kg bw/d, ip) injected from day 11 to day 16. The renal antioxidant enzyme activities reduced glutathione, lipid peroxidation, and serum renal markers. Urea and creatinine levels were estimated. The renal expression of interleukin-1β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and nitric oxide (NO) were determined. Renal levels of inducible nitric oxide synthase (iNOS), nuclear factor-ĸB (NF-ĸB), and caspase-3 were determined to detect possible mechanism of inflammation and apoptosis with histology.Results: MOO prominently reduced serum creatinine and urea levels with amelioration of histopathological abrasions induced by gentamicin (GM). It significantly depressed oxidative stress through lowering of renal malondialdehyde (MDA) and elevation of renal superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) activities, and reduced glutathione (GSH) level. MOO restored renal content of IL-1β, IL-6, TNF-α, and NO, coupled with the mechanistic downregulation of NF-ĸB, iNOS, and caspase-3 activities. The histopathological alterations were ameliorated by MOO.Conclusions: MOO possesses marked nephroprotective effect against GM-induced renal damage via modulating oxidative stress, inflammation, and apoptosis in Wistar rats.

Authors+Show Affiliations

Department of Medical Biochemistry, Faculty of Basic Medicine, Ebonyi State University, Abakaliki, Nigeria.Department of Medical Laboratory Science, Faculty of Health Sciences and Technology, Ebonyi State University, Abakaliki, Nigeria.Department of Medical Biochemistry, Faculty of Basic Medical Sciences, College of Medicine, Alex Ekwueme Federal University, Ikwo, Ebonyi State, Nigeria.Central Research and Diagnostic Laboratory, Ilorin, Kwara State, Nigeria.Department of Medical Biochemistry, Faculty of Basic Medical Sciences, College of Medicine, Alex Ekwueme Federal University, Ikwo, Ebonyi State, Nigeria.Department of Medical Biochemistry, Faculty of Basic Medical Sciences, College of Medicine, Alex Ekwueme Federal University, Ikwo, Ebonyi State, Nigeria.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31403889

Citation

Edeogu, C O., et al. "Nephroprotective Effect of Moringa Oleifera Seed Oil On Gentamicin-Induced Nephrotoxicity in Rats: Biochemical Evaluation of Antioxidant, Anti-inflammatory, and Antiapoptotic Pathways." Journal of the American College of Nutrition, vol. 39, no. 4, 2020, pp. 307-315.
Edeogu CO, Kalu ME, Famurewa AC, et al. Nephroprotective Effect of Moringa Oleifera Seed Oil on Gentamicin-Induced Nephrotoxicity in Rats: Biochemical Evaluation of Antioxidant, Anti-inflammatory, and Antiapoptotic Pathways. J Am Coll Nutr. 2020;39(4):307-315.
Edeogu, C. O., Kalu, M. E., Famurewa, A. C., Asogwa, N. T., Onyeji, G. N., & Ikpemo, K. O. (2020). Nephroprotective Effect of Moringa Oleifera Seed Oil on Gentamicin-Induced Nephrotoxicity in Rats: Biochemical Evaluation of Antioxidant, Anti-inflammatory, and Antiapoptotic Pathways. Journal of the American College of Nutrition, 39(4), 307-315. https://doi.org/10.1080/07315724.2019.1649218
Edeogu CO, et al. Nephroprotective Effect of Moringa Oleifera Seed Oil On Gentamicin-Induced Nephrotoxicity in Rats: Biochemical Evaluation of Antioxidant, Anti-inflammatory, and Antiapoptotic Pathways. J Am Coll Nutr. 2020 May-Jun;39(4):307-315. PubMed PMID: 31403889.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nephroprotective Effect of Moringa Oleifera Seed Oil on Gentamicin-Induced Nephrotoxicity in Rats: Biochemical Evaluation of Antioxidant, Anti-inflammatory, and Antiapoptotic Pathways. AU - Edeogu,C O, AU - Kalu,Michael E, AU - Famurewa,Ademola C, AU - Asogwa,Nnaemeka T, AU - Onyeji,Gertrude N, AU - Ikpemo,Kelechi O, Y1 - 2019/08/12/ PY - 2019/8/14/pubmed PY - 2021/7/15/medline PY - 2019/8/13/entrez KW - Gentamicin KW - Moringa oil KW - apoptosis KW - nephrotoxicity KW - oxidative stress SP - 307 EP - 315 JF - Journal of the American College of Nutrition JO - J Am Coll Nutr VL - 39 IS - 4 N2 - Objective: Gentamicin is an efficacious aminoglycoside antibiotic widely used to treat life-threatening Gram-negative bacteria infections. However, its specific non-targeted induction of nephrotoxicity is a worrying clinical challenge. The study explored the nephroprotective effect of Moringa oleifera seed oil (MOO) against gentamicin-induced oxidative nephrotoxicity, pro-inflammation, and apoptosis in male Wistar rats.Method: Twenty-four rats divided into 4 groups (n = 6) were administered MOO (5 ml/kg) for 16 days and/or gentamicin (100 mg/kg bw/d, ip) injected from day 11 to day 16. The renal antioxidant enzyme activities reduced glutathione, lipid peroxidation, and serum renal markers. Urea and creatinine levels were estimated. The renal expression of interleukin-1β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and nitric oxide (NO) were determined. Renal levels of inducible nitric oxide synthase (iNOS), nuclear factor-ĸB (NF-ĸB), and caspase-3 were determined to detect possible mechanism of inflammation and apoptosis with histology.Results: MOO prominently reduced serum creatinine and urea levels with amelioration of histopathological abrasions induced by gentamicin (GM). It significantly depressed oxidative stress through lowering of renal malondialdehyde (MDA) and elevation of renal superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) activities, and reduced glutathione (GSH) level. MOO restored renal content of IL-1β, IL-6, TNF-α, and NO, coupled with the mechanistic downregulation of NF-ĸB, iNOS, and caspase-3 activities. The histopathological alterations were ameliorated by MOO.Conclusions: MOO possesses marked nephroprotective effect against GM-induced renal damage via modulating oxidative stress, inflammation, and apoptosis in Wistar rats. SN - 1541-1087 UR - https://www.unboundmedicine.com/medline/citation/31403889/Nephroprotective_Effect_of_Moringa_Oleifera_Seed_Oil_on_Gentamicin_Induced_Nephrotoxicity_in_Rats:_Biochemical_Evaluation_of_Antioxidant_Anti_inflammatory_and_Antiapoptotic_Pathways_ L2 - https://www.tandfonline.com/doi/full/10.1080/07315724.2019.1649218 DB - PRIME DP - Unbound Medicine ER -