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Comparative transcriptome analyses reveal genes related to pigmentation in the petals of red and white Primula vulgaris cultivars.
Physiol Mol Biol Plants 2019; 25(4):1029-1041PM

Abstract

Primula vulgaris is an important ornamental plant species with various flower color. To explore the molecular mechanism of its color formation, comparative transcriptome analyses of the petals in red and white cultivars was performed. A total of 4451 differentially expressed genes were identified and annotated into 128 metabolic pathways. Candidate genes FLS, F3'H, DFR, ANS and AOMT in the anthocyanin pathway were expressed significantly higher in the red cultivar than the white and may be responsible for the red coloration. In the red petals, a putative transcription factors bHLH (c52273.graph_c0) was up-regulated about 14-fold, while a R2R3-MYB unigene (c36140.graph_c0) was identified as a repressor involved in anthocyanin regulation and was significantly down-regulated. In addition, the anatomy analyses and pigments composition in the red and white petals were also analyzed. The papillae on the adaxial epidermis of the red petals of P. vulgaris display a triangle-shapes, in contrast with a spherical shape for the white petals. Although flavonoids were detected in both cultivars, anthocyanins could only be identified in the red cultivar. Gossypetin and peonidin/rosinin were the most abundant pigments in red petals. This study shed light on the genetic and biochemistry mechanisms underlying the flower coloration in Primula.

Authors+Show Affiliations

1College of Forestry, Northwest A&F University, Yangling, 712100 Shaanxi China.2College of Landscape Architecture and Art, Northwest A&F University, Yangling, 712100 Shaanxi China.2College of Landscape Architecture and Art, Northwest A&F University, Yangling, 712100 Shaanxi China.Beijing Key Lab of Digital Plant, No. 11 Shuguang Huayuan Middle Road, Haidian District, Beijing, 100097 China.2College of Landscape Architecture and Art, Northwest A&F University, Yangling, 712100 Shaanxi China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31404227

Citation

Li, Long, et al. "Comparative Transcriptome Analyses Reveal Genes Related to Pigmentation in the Petals of Red and White Primula Vulgaris Cultivars." Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology, vol. 25, no. 4, 2019, pp. 1029-1041.
Li L, Zhai Y, Luo X, et al. Comparative transcriptome analyses reveal genes related to pigmentation in the petals of red and white Primula vulgaris cultivars. Physiol Mol Biol Plants. 2019;25(4):1029-1041.
Li, L., Zhai, Y., Luo, X., Zhang, Y., & Shi, Q. (2019). Comparative transcriptome analyses reveal genes related to pigmentation in the petals of red and white Primula vulgaris cultivars. Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology, 25(4), pp. 1029-1041. doi:10.1007/s12298-019-00664-6.
Li L, et al. Comparative Transcriptome Analyses Reveal Genes Related to Pigmentation in the Petals of Red and White Primula Vulgaris Cultivars. Physiol Mol Biol Plants. 2019;25(4):1029-1041. PubMed PMID: 31404227.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative transcriptome analyses reveal genes related to pigmentation in the petals of red and white Primula vulgaris cultivars. AU - Li,Long, AU - Zhai,Yuhui, AU - Luo,Xiaoning, AU - Zhang,Ying, AU - Shi,Qianqian, Y1 - 2019/05/21/ PY - 2018/06/18/received PY - 2019/02/05/revised PY - 2019/04/01/accepted PY - 2020/07/01/pmc-release PY - 2019/8/13/entrez PY - 2019/8/14/pubmed PY - 2019/8/14/medline KW - Anthocyanin biosynthesis KW - Epigenetic analysis KW - Flower coloration KW - Primula vulgaris KW - R2R3-MYB KW - Red petal SP - 1029 EP - 1041 JF - Physiology and molecular biology of plants : an international journal of functional plant biology JO - Physiol Mol Biol Plants VL - 25 IS - 4 N2 - Primula vulgaris is an important ornamental plant species with various flower color. To explore the molecular mechanism of its color formation, comparative transcriptome analyses of the petals in red and white cultivars was performed. A total of 4451 differentially expressed genes were identified and annotated into 128 metabolic pathways. Candidate genes FLS, F3'H, DFR, ANS and AOMT in the anthocyanin pathway were expressed significantly higher in the red cultivar than the white and may be responsible for the red coloration. In the red petals, a putative transcription factors bHLH (c52273.graph_c0) was up-regulated about 14-fold, while a R2R3-MYB unigene (c36140.graph_c0) was identified as a repressor involved in anthocyanin regulation and was significantly down-regulated. In addition, the anatomy analyses and pigments composition in the red and white petals were also analyzed. The papillae on the adaxial epidermis of the red petals of P. vulgaris display a triangle-shapes, in contrast with a spherical shape for the white petals. Although flavonoids were detected in both cultivars, anthocyanins could only be identified in the red cultivar. Gossypetin and peonidin/rosinin were the most abundant pigments in red petals. This study shed light on the genetic and biochemistry mechanisms underlying the flower coloration in Primula. SN - 0971-5894 UR - https://www.unboundmedicine.com/medline/citation/31404227/Comparative_transcriptome_analyses_reveal_genes_related_to_pigmentation_in_the_petals_of_red_and_white_Primula_vulgaris_cultivars DB - PRIME DP - Unbound Medicine ER -
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