Tags

Type your tag names separated by a space and hit enter

The Nucleolus: A Multiphase Condensate Balancing Ribosome Synthesis and Translational Capacity in Health, Aging and Ribosomopathies.
Cells 2019; 8(8)C

Abstract

The nucleolus is the largest membrane-less structure in the eukaryotic nucleus. It is involved in the biogenesis of ribosomes, essential macromolecular machines responsible for synthesizing all proteins required by the cell. The assembly of ribosomes is evolutionarily conserved and is the most energy-consuming cellular process needed for cell growth, proliferation, and homeostasis. Despite the significance of this process, the intricate pathophysiological relationship between the nucleolus and protein synthesis has only recently begun to emerge. Here, we provide perspective on new principles governing nucleolar formation and the resulting multiphase organization driven by liquid-liquid phase separation. With recent advances in the structural analysis of ribosome formation, we highlight the current understanding of the step-wise assembly of pre-ribosomal subunits and the quality control required for proper function. Finally, we address how aging affects ribosome genesis and how genetic defects in ribosome formation cause ribosomopathies, complex diseases with a predisposition to cancer.

Authors+Show Affiliations

Center for Proteomics and Molecular Therapeutics, Rosalind Franklin University of Medicine & Science, North Chicago, IL 60064, USA. carl.correll@rosalindfranklin.edu.Danish Cancer Society Research Center, Genome Integrity Unit, DK-2100 Copenhagen, Denmark. Division of Genome Biology, Department of Medical Biochemistry and Biophysics, Science for Life Laboratory, Karolinska Institute, SE-171 77 Stockholm, Sweden.Center for Cancer Cell Biology Immunology and Infection, Rosalind Franklin University of Medicine & Science, North Chicago, IL 60064, USA. mirek.dundr@rosalindfranklin.edu.

Pub Type(s)

Journal Article
Review

Language

eng

PubMed ID

31405125

Citation

Correll, Carl C., et al. "The Nucleolus: a Multiphase Condensate Balancing Ribosome Synthesis and Translational Capacity in Health, Aging and Ribosomopathies." Cells, vol. 8, no. 8, 2019.
Correll CC, Bartek J, Dundr M. The Nucleolus: A Multiphase Condensate Balancing Ribosome Synthesis and Translational Capacity in Health, Aging and Ribosomopathies. Cells. 2019;8(8).
Correll, C. C., Bartek, J., & Dundr, M. (2019). The Nucleolus: A Multiphase Condensate Balancing Ribosome Synthesis and Translational Capacity in Health, Aging and Ribosomopathies. Cells, 8(8), doi:10.3390/cells8080869.
Correll CC, Bartek J, Dundr M. The Nucleolus: a Multiphase Condensate Balancing Ribosome Synthesis and Translational Capacity in Health, Aging and Ribosomopathies. Cells. 2019 Aug 10;8(8) PubMed PMID: 31405125.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The Nucleolus: A Multiphase Condensate Balancing Ribosome Synthesis and Translational Capacity in Health, Aging and Ribosomopathies. AU - Correll,Carl C, AU - Bartek,Jiri, AU - Dundr,Miroslav, Y1 - 2019/08/10/ PY - 2019/06/14/received PY - 2019/07/31/revised PY - 2019/08/06/accepted PY - 2019/8/14/entrez PY - 2019/8/14/pubmed PY - 2019/8/14/medline KW - genome instability KW - links to diseases KW - nucleolus KW - phase separation KW - pre-rRNA processing KW - rDNA genes KW - ribosomal genes KW - ribosome biogenesis JF - Cells JO - Cells VL - 8 IS - 8 N2 - The nucleolus is the largest membrane-less structure in the eukaryotic nucleus. It is involved in the biogenesis of ribosomes, essential macromolecular machines responsible for synthesizing all proteins required by the cell. The assembly of ribosomes is evolutionarily conserved and is the most energy-consuming cellular process needed for cell growth, proliferation, and homeostasis. Despite the significance of this process, the intricate pathophysiological relationship between the nucleolus and protein synthesis has only recently begun to emerge. Here, we provide perspective on new principles governing nucleolar formation and the resulting multiphase organization driven by liquid-liquid phase separation. With recent advances in the structural analysis of ribosome formation, we highlight the current understanding of the step-wise assembly of pre-ribosomal subunits and the quality control required for proper function. Finally, we address how aging affects ribosome genesis and how genetic defects in ribosome formation cause ribosomopathies, complex diseases with a predisposition to cancer. SN - 2073-4409 UR - https://www.unboundmedicine.com/medline/citation/31405125/The_Nucleolus:_A_Multiphase_Condensate_Balancing_Ribosome_Synthesis_and_Translational_Capacity_in_Health,_Aging_and_Ribosomopathies L2 - http://www.mdpi.com/resolver?pii=cells8080869 DB - PRIME DP - Unbound Medicine ER -
Unbound Prime app for iOS iPhone iPadUnbound PubMed app for AndroidAlso Available:
Unbound MEDLINE
Unbound PubMed app for WindowsUnbound PubMed app for MAC OSX Yosemite Macbook Air pro