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Ovarian and follicular variables used to determine ewes with different FecGE genotypes.
Anim Reprod Sci 2019; 208:106117AR

Abstract

Based on ovarian and follicular variables, there was determination of ewes with different FecGE genotypes. Based on the FecGE genotype, 65 Santa Inês ewes were assigned to three experimental groups: homozygous wild-type (n = 25; FecG+/+), mutant heterozygous (n = 27; FecG+/E) and mutant homozygous (n = 13; FecGE/E). The ewe's ovaries were weighed and measured, then the follicles (oocyte, nucleus and nucleolus) were histologically evaluated for morphometry and morphology. Morphologically normal follicles, in the primordial and transitional stages, explained 70.18% of the variability morphological characteristics between mutant and wild-type ewes. Conducting the morphometric evaluation resulted in a more precise determination of the genotype groups when there was assessment of the primordial and secondary follicular developmental stages. The diameter of the oocyte and the oocyte nucleus of the primordial follicles explained 36.76% of the variability in follicular morphology between ewes with the mutation and those with the wildtype group. Similarly, the core diameter of oocytes in secondary follicles explained 10.63% of the variability in follicular morphology among FecGE/E, FecG+/E and FecG+/+ ewes. Thus, morphologically normal follicles in the primordial and transitional stages of development are the variables that allow for a more precise differentiation of Santa Inês ewes with the FecGE mutation. These variables may be evaluated to make more efficient the adoption of biotechniques that when conducted there is utilisation of follicles in the initial developmental stages as a physiological basis for classifying whether specific follicles are useful when conducting the techniques.

Authors+Show Affiliations

Reproductive Biotechniques Laboratory, Federal Rural University of Pernambuco, Rua Dom, R. Manuel de Medeiros, s/n- Dois Irmãos, 52171-900, Recife-PE, Brazil.Cesmac University Center, Rua da Harmônia- Farol, 57081-350, Macéio-AL, Brazil.Reproductive Biotechniques Laboratory, Federal Rural University of Pernambuco, Rua Dom, R. Manuel de Medeiros, s/n- Dois Irmãos, 52171-900, Recife-PE, Brazil.Brazilian Agricultural Research Corporation - Embrapa Recursos Genéticos e Biotecnologia (Embrapa Genetic Resources and Biotechnology), PqEB s/n Brasília-DF, 70770917, DF, Brazil; Graduation Program in Biotechnology, Federal University of Tocantins (UFT), Rua Bandejós, chácaras 69/72, Zona Rural, Gurupi-TO, 77402-970, Brazil.Graduation Program in Biotechnology, Federal University of Tocantins (UFT), Rua Bandejós, chácaras 69/72, Zona Rural, Gurupi-TO, 77402-970, Brazil.Brazilian Agricultural Research Corporation- Embrapa Gado de Leite (Embrapa Dairy Cattle), Rua Eugênio do Nascimento, 610 - Dom Bosco, 36038-330, Juiz de Fora-MG, Brazil.Reproductive Biotechniques Laboratory, Federal Rural University of Pernambuco, Rua Dom, R. Manuel de Medeiros, s/n- Dois Irmãos, 52171-900, Recife-PE, Brazil.Brazilian Agricultural Research Corporation - Embrapa Tabuleiros Costeiros (Embrapa Coastal Tablelands), Avenida Beira Mar, 3250 Jardins, 49025-040, Aracaju-SE, Brazil. Electronic address: hymerson.azevedo@embrapa.br.Reproductive Biotechniques Laboratory, Federal Rural University of Pernambuco, Rua Dom, R. Manuel de Medeiros, s/n- Dois Irmãos, 52171-900, Recife-PE, Brazil.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31405468

Citation

Chaves, Maiana Silva, et al. "Ovarian and Follicular Variables Used to Determine Ewes With Different FecGE Genotypes." Animal Reproduction Science, vol. 208, 2019, p. 106117.
Chaves MS, Luz VB, Ferreira-Silva JC, et al. Ovarian and follicular variables used to determine ewes with different FecGE genotypes. Anim Reprod Sci. 2019;208:106117.
Chaves, M. S., Luz, V. B., Ferreira-Silva, J. C., de Oliveira Melo, E., Paiva, S. R., de Barros, I., ... Lemos Oliveira, M. A. (2019). Ovarian and follicular variables used to determine ewes with different FecGE genotypes. Animal Reproduction Science, 208, p. 106117. doi:10.1016/j.anireprosci.2019.106117.
Chaves MS, et al. Ovarian and Follicular Variables Used to Determine Ewes With Different FecGE Genotypes. Anim Reprod Sci. 2019;208:106117. PubMed PMID: 31405468.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ovarian and follicular variables used to determine ewes with different FecGE genotypes. AU - Chaves,Maiana Silva, AU - Luz,Valesca Barreto, AU - Ferreira-Silva,José Carlos, AU - de Oliveira Melo,Eduardo, AU - Paiva,Samuel Rezende, AU - de Barros,Inácio, AU - Bartolomeu,Cláudio Coutinho, AU - Azevedo,Hymerson Costa, AU - Lemos Oliveira,Marcos Antonio, Y1 - 2019/07/03/ PY - 2019/01/03/received PY - 2019/06/20/revised PY - 2019/07/02/accepted PY - 2019/8/14/entrez KW - GDF-9 KW - Ovine KW - Ovulation KW - Polymorphism KW - Prolificacy SP - 106117 EP - 106117 JF - Animal reproduction science JO - Anim. Reprod. Sci. VL - 208 N2 - Based on ovarian and follicular variables, there was determination of ewes with different FecGE genotypes. Based on the FecGE genotype, 65 Santa Inês ewes were assigned to three experimental groups: homozygous wild-type (n = 25; FecG+/+), mutant heterozygous (n = 27; FecG+/E) and mutant homozygous (n = 13; FecGE/E). The ewe's ovaries were weighed and measured, then the follicles (oocyte, nucleus and nucleolus) were histologically evaluated for morphometry and morphology. Morphologically normal follicles, in the primordial and transitional stages, explained 70.18% of the variability morphological characteristics between mutant and wild-type ewes. Conducting the morphometric evaluation resulted in a more precise determination of the genotype groups when there was assessment of the primordial and secondary follicular developmental stages. The diameter of the oocyte and the oocyte nucleus of the primordial follicles explained 36.76% of the variability in follicular morphology between ewes with the mutation and those with the wildtype group. Similarly, the core diameter of oocytes in secondary follicles explained 10.63% of the variability in follicular morphology among FecGE/E, FecG+/E and FecG+/+ ewes. Thus, morphologically normal follicles in the primordial and transitional stages of development are the variables that allow for a more precise differentiation of Santa Inês ewes with the FecGE mutation. These variables may be evaluated to make more efficient the adoption of biotechniques that when conducted there is utilisation of follicles in the initial developmental stages as a physiological basis for classifying whether specific follicles are useful when conducting the techniques. SN - 1873-2232 UR - https://www.unboundmedicine.com/medline/citation/31405468/Ovarian_and_follicular_variables_used_to_determine_ewes_with_different_FecGE_genotypes L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-4320(19)30009-0 DB - PRIME DP - Unbound Medicine ER -