Tags

Type your tag names separated by a space and hit enter

Serologic Evidence for Orientia Exposure in the Democratic Republic of Sao Tome and Principe.
Vector Borne Zoonotic Dis. 2019 11; 19(11):821-827.VB

Abstract

Orientia tsutsugamushi is an obligate intracellular bacterium that causes scrub typhus in humans. Formerly thought to be confined to the "tsutsugamushi triangle" within the Asia-Pacific region, scrub typhus was recently identified in the Western Hemisphere. Moreover, a new species of Orientia bacterial genus was isolated from a patient in Dubai. This study investigated Orientia exposure in an African country, the Democratic Republic of Sao Tome and Principe. Two sets of samples were analyzed in the study: 240 dried blood spots (DBSs) collected in 2016 and 863 serum samples from 570 pregnant women in 2003. Antibodies against O. tsutsugamushi were examined by immunofluorescence assay (IFA) and enzyme-linked immunosorbent assay (ELISA). The positive samples were further confirmed by Western blot. The results of IFA showed that 5.8% (14/240) of DBSs and 20.4% (116/570) of the serum samples contained reactive antibodies, whereas IgG ELISA yielded a positive rate of 15.4% (88/570) for the serum samples. These findings provided serologic evidence of potential Orientia exposure even though case of scrub typhus has never been diagnosed in the nation. Further studies are needed to determine the epidemiology and the burden of this neglected tropical disease in Africa.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Yen TY
Institute of Epidemiology and Preventive Medicine, College of Public Health, National Taiwan University, Taipei, Taiwan.
Zhang Z
Department of Viral and Rickettsial Diseases, Infectious Diseases Directorate, Naval Medical Research Center, Silver Spring, Maryland. Department of Preventive Medicine and Biostatistics, Uniformed Services University of the Health Sciences, Bethesda, Maryland.
Chao CC
Department of Viral and Rickettsial Diseases, Infectious Diseases Directorate, Naval Medical Research Center, Silver Spring, Maryland. Department of Preventive Medicine and Biostatistics, Uniformed Services University of the Health Sciences, Bethesda, Maryland.
Ching WM
Department of Viral and Rickettsial Diseases, Infectious Diseases Directorate, Naval Medical Research Center, Silver Spring, Maryland. Department of Preventive Medicine and Biostatistics, Uniformed Services University of the Health Sciences, Bethesda, Maryland.
Shu PY
Research and Diagnostic Center, Center for Disease Control, The Ministry of Health and Welfare, Taipei, Taiwan.
Tseng LF
Taiwan Anti-Malaria Advisory Mission, Sao Tome, Democratic Republic of Sao Tome and Principe.
Carvalho AVA
Centro National de Endemias, Sao Tome, Democratic Republic of Sao Tome and Principe.
Tsai KH
Taiwan Anti-Malaria Advisory Mission, Sao Tome, Democratic Republic of Sao Tome and Principe. Institute of Environmental Health, College of Public Health, National Taiwan University, Taipei, Taiwan. Department of Public Health, College of Public Health, National Taiwan University, Taipei, Taiwan.

MeSH

AdolescentAdultAgedAntibodies, BacterialChildChild, PreschoolEnzyme-Linked Immunosorbent AssayFemaleFluorescent Antibody TechniqueHumansImmunoglobulin GImmunoglobulin MInfantMiddle AgedNeglected DiseasesOrientia tsutsugamushiPregnancySao Tome and PrincipeScrub TyphusSeroepidemiologic Studies

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

31407963

Citation

Yen, Tsai-Ying, et al. "Serologic Evidence for Orientia Exposure in the Democratic Republic of Sao Tome and Principe." Vector Borne and Zoonotic Diseases (Larchmont, N.Y.), vol. 19, no. 11, 2019, pp. 821-827.
Yen TY, Zhang Z, Chao CC, et al. Serologic Evidence for Orientia Exposure in the Democratic Republic of Sao Tome and Principe. Vector Borne Zoonotic Dis. 2019;19(11):821-827.
Yen, T. Y., Zhang, Z., Chao, C. C., Ching, W. M., Shu, P. Y., Tseng, L. F., Carvalho, A. V. A., & Tsai, K. H. (2019). Serologic Evidence for Orientia Exposure in the Democratic Republic of Sao Tome and Principe. Vector Borne and Zoonotic Diseases (Larchmont, N.Y.), 19(11), 821-827. https://doi.org/10.1089/vbz.2018.2426
Yen TY, et al. Serologic Evidence for Orientia Exposure in the Democratic Republic of Sao Tome and Principe. Vector Borne Zoonotic Dis. 2019;19(11):821-827. PubMed PMID: 31407963.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Serologic Evidence for Orientia Exposure in the Democratic Republic of Sao Tome and Principe. AU - Yen,Tsai-Ying, AU - Zhang,Zhiwen, AU - Chao,Chien-Chung, AU - Ching,Wei-Mei, AU - Shu,Pei-Yun, AU - Tseng,Lien-Feng, AU - Carvalho,Arlindo Vicente de Assunção, AU - Tsai,Kun-Hsien, Y1 - 2019/08/13/ PY - 2019/8/14/pubmed PY - 2020/9/4/medline PY - 2019/8/14/entrez KW - Democratic Republic of Sao Tome and Principe KW - Orientia tsutsugamushi KW - scrub typhus KW - seroprevalence SP - 821 EP - 827 JF - Vector borne and zoonotic diseases (Larchmont, N.Y.) JO - Vector Borne Zoonotic Dis VL - 19 IS - 11 N2 - Orientia tsutsugamushi is an obligate intracellular bacterium that causes scrub typhus in humans. Formerly thought to be confined to the "tsutsugamushi triangle" within the Asia-Pacific region, scrub typhus was recently identified in the Western Hemisphere. Moreover, a new species of Orientia bacterial genus was isolated from a patient in Dubai. This study investigated Orientia exposure in an African country, the Democratic Republic of Sao Tome and Principe. Two sets of samples were analyzed in the study: 240 dried blood spots (DBSs) collected in 2016 and 863 serum samples from 570 pregnant women in 2003. Antibodies against O. tsutsugamushi were examined by immunofluorescence assay (IFA) and enzyme-linked immunosorbent assay (ELISA). The positive samples were further confirmed by Western blot. The results of IFA showed that 5.8% (14/240) of DBSs and 20.4% (116/570) of the serum samples contained reactive antibodies, whereas IgG ELISA yielded a positive rate of 15.4% (88/570) for the serum samples. These findings provided serologic evidence of potential Orientia exposure even though case of scrub typhus has never been diagnosed in the nation. Further studies are needed to determine the epidemiology and the burden of this neglected tropical disease in Africa. SN - 1557-7759 UR - https://www.unboundmedicine.com/medline/citation/31407963/Serologic_Evidence_for_Orientia_Exposure_in_the_Democratic_Republic_of_Sao_Tome_and_Principe_ DB - PRIME DP - Unbound Medicine ER -