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Pol5 is an essential ribosome biogenesis factor required for 60S ribosomal subunit maturation in Saccharomyces cerevisiae.
RNA. 2019 11; 25(11):1561-1575.RNA

Abstract

In Saccharomyces cerevisiae, more than 250 trans-acting factors are involved in the maturation of 40S and 60S ribosomal subunits. The expression of most of these factors is transcriptionally coregulated to ensure correct ribosome production under a wide variety of environmental and intracellular conditions. Here, we identified the essential nucleolar Pol5 protein as a novel trans-acting factor required for the synthesis of 60S ribosomal subunits. Pol5 weakly and/or transiently associates with early to medium pre-60S ribosomal particles. Depletion of and temperature-sensitive mutations in Pol5 result in a deficiency of 60S ribosomal subunits and accumulation of half-mer polysomes. Both processing of 27SB pre-rRNA to mature 25S rRNA and release of pre-60S ribosomal particles from the nucle(ol)us to the cytoplasm are impaired in the Pol5-depleted strain. Moreover, we identified the genes encoding ribosomal proteins uL23 and eL27A as multicopy suppressors of the slow growth of a temperature-sensitive pol5 mutant. These results suggest that Pol5 could function in ensuring the correct folding of 25S rRNA domain III; thus, favoring the correct assembly of these two ribosomal proteins at their respective binding sites into medium pre-60S ribosomal particles. Pol5 is homologous to the human tumor suppressor Myb-binding protein 1A (MYBBP1A). However, expression of MYBBP1A failed to complement the lethal phenotype of a pol5 null mutant strain though interfered with 60S ribosomal subunit biogenesis.

Authors+Show Affiliations

Instituto de Biomedicina de Sevilla, Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla, E-41013, Seville, Spain. Departamento de Genética, Facultad de Biología, Universidad de Sevilla, E-41012, Seville, Spain.Instituto de Biomedicina de Sevilla, Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla, E-41013, Seville, Spain. Departamento de Genética, Facultad de Biología, Universidad de Sevilla, E-41012, Seville, Spain.Instituto de Biomedicina de Sevilla, Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla, E-41013, Seville, Spain. Departamento de Genética, Facultad de Biología, Universidad de Sevilla, E-41012, Seville, Spain.Instituto de Biomedicina de Sevilla, Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla, E-41013, Seville, Spain.Centro de Investigación del Cáncer and Instituto de Biología Molecular y Celular del Cáncer, CSIC-Universidad de Salamanca, E-37007, Salamanca, Spain. Centro de Investigación Biomédica en Red en Cáncer (CIBERONC), CSIC-Universidad de Salamanca, E-37007, Salamanca, Spain.Instituto de Biomedicina de Sevilla, Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla, E-41013, Seville, Spain. Departamento de Microbiología, Facultad de Biología, Universidad de Sevilla, E-41012, Seville, Spain.Centro de Investigación del Cáncer and Instituto de Biología Molecular y Celular del Cáncer, CSIC-Universidad de Salamanca, E-37007, Salamanca, Spain. Centro de Investigación Biomédica en Red en Cáncer (CIBERONC), CSIC-Universidad de Salamanca, E-37007, Salamanca, Spain. Departamento de Bioquímica y Biología Molecular, Universidad de Salamanca, E-37007, Salamanca, Spain.Instituto de Biomedicina de Sevilla, Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla, E-41013, Seville, Spain. Departamento de Genética, Facultad de Biología, Universidad de Sevilla, E-41012, Seville, Spain.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31413149

Citation

Ramos-Sáenz, Ana, et al. "Pol5 Is an Essential Ribosome Biogenesis Factor Required for 60S Ribosomal Subunit Maturation in Saccharomyces Cerevisiae." RNA (New York, N.Y.), vol. 25, no. 11, 2019, pp. 1561-1575.
Ramos-Sáenz A, González-Álvarez D, Rodríguez-Galán O, et al. Pol5 is an essential ribosome biogenesis factor required for 60S ribosomal subunit maturation in Saccharomyces cerevisiae. RNA. 2019;25(11):1561-1575.
Ramos-Sáenz, A., González-Álvarez, D., Rodríguez-Galán, O., Rodríguez-Gil, A., Gaspar, S. G., Villalobo, E., Dosil, M., & de la Cruz, J. (2019). Pol5 is an essential ribosome biogenesis factor required for 60S ribosomal subunit maturation in Saccharomyces cerevisiae. RNA (New York, N.Y.), 25(11), 1561-1575. https://doi.org/10.1261/rna.072116.119
Ramos-Sáenz A, et al. Pol5 Is an Essential Ribosome Biogenesis Factor Required for 60S Ribosomal Subunit Maturation in Saccharomyces Cerevisiae. RNA. 2019;25(11):1561-1575. PubMed PMID: 31413149.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pol5 is an essential ribosome biogenesis factor required for 60S ribosomal subunit maturation in Saccharomyces cerevisiae. AU - Ramos-Sáenz,Ana, AU - González-Álvarez,Daniel, AU - Rodríguez-Galán,Olga, AU - Rodríguez-Gil,Alfonso, AU - Gaspar,Sonia G, AU - Villalobo,Eduardo, AU - Dosil,Mercedes, AU - de la Cruz,Jesús, Y1 - 2019/08/14/ PY - 2019/05/24/received PY - 2019/08/08/accepted PY - 2020/11/01/pmc-release PY - 2019/8/16/pubmed PY - 2020/1/7/medline PY - 2019/8/16/entrez KW - MYBBP1A KW - nucleolus KW - pre-rRNA processing KW - ribosomal proteins uL23 and eL27 KW - ribosome biogenesis SP - 1561 EP - 1575 JF - RNA (New York, N.Y.) JO - RNA VL - 25 IS - 11 N2 - In Saccharomyces cerevisiae, more than 250 trans-acting factors are involved in the maturation of 40S and 60S ribosomal subunits. The expression of most of these factors is transcriptionally coregulated to ensure correct ribosome production under a wide variety of environmental and intracellular conditions. Here, we identified the essential nucleolar Pol5 protein as a novel trans-acting factor required for the synthesis of 60S ribosomal subunits. Pol5 weakly and/or transiently associates with early to medium pre-60S ribosomal particles. Depletion of and temperature-sensitive mutations in Pol5 result in a deficiency of 60S ribosomal subunits and accumulation of half-mer polysomes. Both processing of 27SB pre-rRNA to mature 25S rRNA and release of pre-60S ribosomal particles from the nucle(ol)us to the cytoplasm are impaired in the Pol5-depleted strain. Moreover, we identified the genes encoding ribosomal proteins uL23 and eL27A as multicopy suppressors of the slow growth of a temperature-sensitive pol5 mutant. These results suggest that Pol5 could function in ensuring the correct folding of 25S rRNA domain III; thus, favoring the correct assembly of these two ribosomal proteins at their respective binding sites into medium pre-60S ribosomal particles. Pol5 is homologous to the human tumor suppressor Myb-binding protein 1A (MYBBP1A). However, expression of MYBBP1A failed to complement the lethal phenotype of a pol5 null mutant strain though interfered with 60S ribosomal subunit biogenesis. SN - 1469-9001 UR - https://www.unboundmedicine.com/medline/citation/31413149/Pol5_is_an_essential_ribosome_biogenesis_factor_required_for_60S_ribosomal_subunit_maturation_in_Saccharomyces_cerevisiae L2 - http://www.rnajournal.org/cgi/pmidlookup?view=long&pmid=31413149 DB - PRIME DP - Unbound Medicine ER -