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Effect of acute exercise on RBC deformability and RBC nitric oxide synthase signalling pathway in young sickle cell anaemia patients.
Sci Rep. 2019 08 14; 9(1):11813.SR

Abstract

Sickle cell anaemia (SCA) is characterized by reduced red blood cell (RBC) deformability and nitric oxide (NO) bioavailability. The aim of the study was to investigate whether exercise might affect these parameters in SCA. SCA patients and healthy controls (AA) performed an acute submaximal exercise test until subjects reached the first ventilatory threshold (VT 1). Blood was sampled at rest and at VT 1. At rest, free haemoglobin level was higher and RBC count, haemoglobin and haematocrit were lower in SCA compared to AA. RBC deformability was lower in SCA. Exercise had no effect on the tested parameters. RBC NO level was higher in SCA compared to AA at rest and significantly decreased after exercise in SCA. This might be related to a reduction in RBC-NO synthase (RBC-NOS) activation which was only observed in SCA after exercise. Free radical levels were higher in SCA at rest but concentration was not affected by exercise. Marker for lipid peroxidation and antioxidative capacity were similar in SCA and AA and not affected by exercise. In conclusion, a single acute submaximal bout of exercise has no deleterious effects on RBC deformability or oxidative stress markers in SCA, and seems to modulate RBC-NOS signalling pathway.

Links

Authors+Show Affiliations

German Sport University Cologne, Department of Molecular and Cellular Sports Medicine, Cologne, Germany. m.grau@dshs-koeln.de.

German Sport University Cologne, Department of Molecular and Cellular Sports Medicine, Cologne, Germany.

University of Lyon, University Claude Bernard Lyon 1, Interuniversity Laboratory of Human Movement Biology EA7424, "Vascular Biology and Red Blood Cell" team, Villeurbanne, France. Laboratory of Excellence "GR-Ex", Paris, France.

German Sport University Cologne, Department of Molecular and Cellular Sports Medicine, Cologne, Germany.

University of Lyon, University Claude Bernard Lyon 1, Interuniversity Laboratory of Human Movement Biology EA7424, "Vascular Biology and Red Blood Cell" team, Villeurbanne, France. Laboratory of Excellence "GR-Ex", Paris, France. East Biology Centre, UF "Biochemistry of Red Blood Cell Disease", Academic Hospital of Lyon, HCL, Lyon, France.

German Sport University Cologne, Department of Molecular and Cellular Sports Medicine, Cologne, Germany.

German Sport University Cologne, Department of Molecular and Cellular Sports Medicine, Cologne, Germany.

German Sport University Cologne, Department of Molecular and Cellular Sports Medicine, Cologne, Germany.

University of Lyon, University Claude Bernard Lyon 1, Interuniversity Laboratory of Human Movement Biology EA7424, "Vascular Biology and Red Blood Cell" team, Villeurbanne, France. Laboratory of Excellence "GR-Ex", Paris, France. East Biology Centre, UF "Biochemistry of Red Blood Cell Disease", Academic Hospital of Lyon, HCL, Lyon, France.

German Sport University Cologne, Department of Molecular and Cellular Sports Medicine, Cologne, Germany.

University of Lyon, University Claude Bernard Lyon 1, Interuniversity Laboratory of Human Movement Biology EA7424, "Vascular Biology and Red Blood Cell" team, Villeurbanne, France. Laboratory of Excellence "GR-Ex", Paris, France.

Children's Hospital Amsterdamer Straβe Cologne; Clinic for Children and Youth Medicine, Paediatric Oncology/Haematology, Cologne, Germany.

MeSH

AdolescentAnemia, Sickle CellCase-Control StudiesErythrocyte DeformabilityErythrocytesExerciseFemaleHumansMaleNitric Oxide SynthaseOxidative StressSignal Transduction

Pub Type(s)

Journal Article

Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31413300

Citation

Grau, Marijke, et al. "Effect of Acute Exercise On RBC Deformability and RBC Nitric Oxide Synthase Signalling Pathway in Young Sickle Cell Anaemia Patients." Scientific Reports, vol. 9, no. 1, 2019, p. 11813.

Grau M, Jerke M, Nader E, et al. Effect of acute exercise on RBC deformability and RBC nitric oxide synthase signalling pathway in young sickle cell anaemia patients. Sci Rep. 2019;9(1):11813.

Grau, M., Jerke, M., Nader, E., Schenk, A., Renoux, C., Collins, B., Dietz, T., Bizjak, D. A., Joly, P., Bloch, W., Connes, P., & Prokop, A. (2019). Effect of acute exercise on RBC deformability and RBC nitric oxide synthase signalling pathway in young sickle cell anaemia patients. Scientific Reports, 9(1), 11813. https://doi.org/10.1038/s41598-019-48364-1

Grau M, et al. Effect of Acute Exercise On RBC Deformability and RBC Nitric Oxide Synthase Signalling Pathway in Young Sickle Cell Anaemia Patients. Sci Rep. 2019 08 14;9(1):11813. PubMed PMID: 31413300.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Effect of acute exercise on RBC deformability and RBC nitric oxide synthase signalling pathway in young sickle cell anaemia patients. AU - Grau,Marijke, AU - Jerke,Max, AU - Nader,Elie, AU - Schenk,Alexander, AU - Renoux,Celine, AU - Collins,Bianca, AU - Dietz,Thomas, AU - Bizjak,Daniel Alexander, AU - Joly,Philippe, AU - Bloch,Wilhelm, AU - Connes,Philippe, AU - Prokop,Aram, Y1 - 2019/08/14/ PY - 2019/06/07/received PY - 2019/08/02/accepted PY - 2019/8/16/entrez PY - 2019/8/16/pubmed PY - 2020/11/11/medline SP - 11813 EP - 11813 JF - Scientific reports JO - Sci Rep VL - 9 IS - 1 N2 - Sickle cell anaemia (SCA) is characterized by reduced red blood cell (RBC) deformability and nitric oxide (NO) bioavailability. The aim of the study was to investigate whether exercise might affect these parameters in SCA. SCA patients and healthy controls (AA) performed an acute submaximal exercise test until subjects reached the first ventilatory threshold (VT 1). Blood was sampled at rest and at VT 1. At rest, free haemoglobin level was higher and RBC count, haemoglobin and haematocrit were lower in SCA compared to AA. RBC deformability was lower in SCA. Exercise had no effect on the tested parameters. RBC NO level was higher in SCA compared to AA at rest and significantly decreased after exercise in SCA. This might be related to a reduction in RBC-NO synthase (RBC-NOS) activation which was only observed in SCA after exercise. Free radical levels were higher in SCA at rest but concentration was not affected by exercise. Marker for lipid peroxidation and antioxidative capacity were similar in SCA and AA and not affected by exercise. In conclusion, a single acute submaximal bout of exercise has no deleterious effects on RBC deformability or oxidative stress markers in SCA, and seems to modulate RBC-NOS signalling pathway. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/31413300/Effect_of_acute_exercise_on_RBC_deformability_and_RBC_nitric_oxide_synthase_signalling_pathway_in_young_sickle_cell_anaemia_patients_ DB - PRIME DP - Unbound Medicine ER -