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Evaluation of carbon sources for the production of inulinase by Aspergillus niger A42 and its characterization.
Bioprocess Biosyst Eng. 2019 Dec; 42(12):1993-2005.BB

Abstract

Inulinases are used for the production of high-fructose syrup and fructooligosaccharides, and are widely utilized in food and pharmaceutical industries. In this study, different carbon sources were screened for inulinase production by Aspergillus niger in shake flask fermentation. Optimum working conditions of the enzyme were determined. Additionally, some properties of produced enzyme were determined [activation (Ea)/inactivation (Eia) energies, Q10 value, inactivation rate constant (kd), half-life (t1/2), D value, Z value, enthalpy (ΔH), free energy (ΔG), and entropy (ΔS)]. Results showed that sugar beet molasses (SBM) was the best in the production of inulinase, which gave 383.73 U/mL activity at 30 °C, 200 rpm and initial pH 5.0 for 10 days with 2% (v/v) of the prepared spore solution. Optimum working conditions were 4.8 pH, 60 °C, and 10 min, which yielded 604.23 U/mL, 1.09 inulinase/sucrase ratio, and 2924.39 U/mg. Additionally, Ea and Eia of inulinase reaction were 37.30 and 112.86 kJ/mol, respectively. Beyond 60 °C, Q10 values of inulinase dropped below one. At 70 and 80 °C, t1/2 of inulinase was 33.6 and 7.2 min; therefore, inulinase is unstable at high temperatures, respectively. Additionally, t1/2, D, ΔH, ΔG values of inulinase decreased with the increase in temperature. Z values of inulinase were 7.21 °C. Negative values of ΔS showed that enzymes underwent a significant process of aggregation during denaturation. Consequently, SBM is a promising carbon source for inulinase production by A. niger. Also, this is the first report on the determination of some properties of A. niger A42 (ATCC 204,447) inulinase.

Authors+Show Affiliations

Department of Food Engineering, Akdeniz University, 07058, Antalya, Turkey.Department of Food Engineering, Akdeniz University, 07058, Antalya, Turkey. iturhan@akdeniz.edu.tr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31414183

Citation

Germec, Mustafa, and Irfan Turhan. "Evaluation of Carbon Sources for the Production of Inulinase By Aspergillus Niger A42 and Its Characterization." Bioprocess and Biosystems Engineering, vol. 42, no. 12, 2019, pp. 1993-2005.
Germec M, Turhan I. Evaluation of carbon sources for the production of inulinase by Aspergillus niger A42 and its characterization. Bioprocess Biosyst Eng. 2019;42(12):1993-2005.
Germec, M., & Turhan, I. (2019). Evaluation of carbon sources for the production of inulinase by Aspergillus niger A42 and its characterization. Bioprocess and Biosystems Engineering, 42(12), 1993-2005. https://doi.org/10.1007/s00449-019-02192-9
Germec M, Turhan I. Evaluation of Carbon Sources for the Production of Inulinase By Aspergillus Niger A42 and Its Characterization. Bioprocess Biosyst Eng. 2019;42(12):1993-2005. PubMed PMID: 31414183.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of carbon sources for the production of inulinase by Aspergillus niger A42 and its characterization. AU - Germec,Mustafa, AU - Turhan,Irfan, Y1 - 2019/08/15/ PY - 2019/04/18/received PY - 2019/08/07/accepted PY - 2019/8/16/pubmed PY - 2020/4/10/medline PY - 2019/8/16/entrez KW - Activation energy KW - Aspergillus niger KW - Inulinase KW - Sucrose KW - Thermal inactivation KW - Thermodynamics SP - 1993 EP - 2005 JF - Bioprocess and biosystems engineering JO - Bioprocess Biosyst Eng VL - 42 IS - 12 N2 - Inulinases are used for the production of high-fructose syrup and fructooligosaccharides, and are widely utilized in food and pharmaceutical industries. In this study, different carbon sources were screened for inulinase production by Aspergillus niger in shake flask fermentation. Optimum working conditions of the enzyme were determined. Additionally, some properties of produced enzyme were determined [activation (Ea)/inactivation (Eia) energies, Q10 value, inactivation rate constant (kd), half-life (t1/2), D value, Z value, enthalpy (ΔH), free energy (ΔG), and entropy (ΔS)]. Results showed that sugar beet molasses (SBM) was the best in the production of inulinase, which gave 383.73 U/mL activity at 30 °C, 200 rpm and initial pH 5.0 for 10 days with 2% (v/v) of the prepared spore solution. Optimum working conditions were 4.8 pH, 60 °C, and 10 min, which yielded 604.23 U/mL, 1.09 inulinase/sucrase ratio, and 2924.39 U/mg. Additionally, Ea and Eia of inulinase reaction were 37.30 and 112.86 kJ/mol, respectively. Beyond 60 °C, Q10 values of inulinase dropped below one. At 70 and 80 °C, t1/2 of inulinase was 33.6 and 7.2 min; therefore, inulinase is unstable at high temperatures, respectively. Additionally, t1/2, D, ΔH, ΔG values of inulinase decreased with the increase in temperature. Z values of inulinase were 7.21 °C. Negative values of ΔS showed that enzymes underwent a significant process of aggregation during denaturation. Consequently, SBM is a promising carbon source for inulinase production by A. niger. Also, this is the first report on the determination of some properties of A. niger A42 (ATCC 204,447) inulinase. SN - 1615-7605 UR - https://www.unboundmedicine.com/medline/citation/31414183/Evaluation_of_carbon_sources_for_the_production_of_inulinase_by_Aspergillus_niger_A42_and_its_characterization L2 - https://dx.doi.org/10.1007/s00449-019-02192-9 DB - PRIME DP - Unbound Medicine ER -