Tags

Type your tag names separated by a space and hit enter

Cloning, expression & evaluation of potential immunogenic recombinant capsid premembrane protein of West Nile virus.
Indian J Med Res 2019; 149(5):656-661IJ

Abstract

Background & objectives

West Nile virus (WNV) is a neurotropic flavivirus that has emerged globally as a significant cause of viral encephalitis. The early confirmatory diagnosis of WNV infections is important for timely clinical management and in areas where multiple flaviviruses are endemic. Diagnosis of WNV infection is primarily based on serodiagnosis, followed by virus isolation and identification. The aim of this study was to develop and evaluate a highly sensitive and specific immunoglobulin M (IgM) ELISA using the recombinant CprM protein (rWNV-CprM) for rapid, early and accurate diagnosis of WNV.

Methods

The gene coding for the CprM protein of WNV was cloned and expressed in pET 28a vector followed by purification. An indirect IgM microplate ELISA using purified rWNV-CprM protein was optimized having no cross-reactivity with healthy human serum and serum samples obtained from patients with dengue and Japanese encephalitis viruses infection.

Results

The comparative evaluation of this rWNV-CprM protein-specific IgM ELISA with plaque reduction neutralization test using 105 blood samples collected from patients suspected to have acute WNV infection revealed 98 per cent concordance with sensitivity and specificity of 100 and 97 per cent, respectively.

Interpretation & conclusions

The recombinant CprM protein-based WNV-specific ELISA reported in this study may be useful for rapid screening of large numbers of blood samples in endemic areas during outbreaks.

Authors+Show Affiliations

Division of Virology, Defence Research & Development Establishment, Gwalior, India.Department of Ophthalmology, Aravind Eye Hospital, Madurai, India.Division of Virology, Defence Research & Development Establishment, Gwalior, India.Division of Virology, Defence Research & Development Establishment, Gwalior, India.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31417034

Citation

Kumar, Jyoti S., et al. "Cloning, Expression & Evaluation of Potential Immunogenic Recombinant Capsid Premembrane Protein of West Nile Virus." The Indian Journal of Medical Research, vol. 149, no. 5, 2019, pp. 656-661.
Kumar JS, Rathinam S, Karothia D, et al. Cloning, expression & evaluation of potential immunogenic recombinant capsid premembrane protein of West Nile virus. Indian J Med Res. 2019;149(5):656-661.
Kumar, J. S., Rathinam, S., Karothia, D., & Parida, M. (2019). Cloning, expression & evaluation of potential immunogenic recombinant capsid premembrane protein of West Nile virus. The Indian Journal of Medical Research, 149(5), pp. 656-661. doi:10.4103/ijmr.IJMR_305_17.
Kumar JS, et al. Cloning, Expression & Evaluation of Potential Immunogenic Recombinant Capsid Premembrane Protein of West Nile Virus. Indian J Med Res. 2019;149(5):656-661. PubMed PMID: 31417034.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cloning, expression & evaluation of potential immunogenic recombinant capsid premembrane protein of West Nile virus. AU - Kumar,Jyoti S, AU - Rathinam,Sivakumar, AU - Karothia,Divanyshi, AU - Parida,Manmohan, PY - 2019/8/17/entrez PY - 2019/8/17/pubmed PY - 2019/8/17/medline KW - CprM gene KW - ELISA KW - blood samples KW - plaque reduction neutralization test KW - recombinant protein - West Nile virus SP - 656 EP - 661 JF - The Indian journal of medical research JO - Indian J. Med. Res. VL - 149 IS - 5 N2 - Background & objectives: West Nile virus (WNV) is a neurotropic flavivirus that has emerged globally as a significant cause of viral encephalitis. The early confirmatory diagnosis of WNV infections is important for timely clinical management and in areas where multiple flaviviruses are endemic. Diagnosis of WNV infection is primarily based on serodiagnosis, followed by virus isolation and identification. The aim of this study was to develop and evaluate a highly sensitive and specific immunoglobulin M (IgM) ELISA using the recombinant CprM protein (rWNV-CprM) for rapid, early and accurate diagnosis of WNV. Methods: The gene coding for the CprM protein of WNV was cloned and expressed in pET 28a vector followed by purification. An indirect IgM microplate ELISA using purified rWNV-CprM protein was optimized having no cross-reactivity with healthy human serum and serum samples obtained from patients with dengue and Japanese encephalitis viruses infection. Results: The comparative evaluation of this rWNV-CprM protein-specific IgM ELISA with plaque reduction neutralization test using 105 blood samples collected from patients suspected to have acute WNV infection revealed 98 per cent concordance with sensitivity and specificity of 100 and 97 per cent, respectively. Interpretation & conclusions: The recombinant CprM protein-based WNV-specific ELISA reported in this study may be useful for rapid screening of large numbers of blood samples in endemic areas during outbreaks. SN - 0971-5916 UR - https://www.unboundmedicine.com/medline/citation/31417034/Cloning,_expression__evaluation_of_potential_immunogenic_recombinant_capsid_premembrane_protein_of_West_Nile_virus L2 - http://www.ijmr.org.in/article.asp?issn=0971-5916;year=2019;volume=149;issue=5;spage=656;epage=661;aulast=Kumar DB - PRIME DP - Unbound Medicine ER -