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Enhanced cellulase production by decreasing intercellular pH through H+-ATPase gene deletion in Trichoderma reesei RUT-C30.
Biotechnol Biofuels 2019; 12:195BB

Abstract

Background

Cellulolytic enzymes produced by Trichoderma reesei are widely used for the industrial production of biofuels and chemicals from lignocellulose. We speculated that intracellular pH during the fermentation process can affect cellulase induction.

Results

In this study, two H+-ATPase genes, tre76238 and tre78757, were first identified in T. reesei. Deletion of tre76238 and tre78757 in T. reesei RUT-C30 confirmed that tre76238 has a major function in maintaining intracellular pH, whereas tre78757 has a minor function. The tre76238 deletion strain Δ76238 displayed a high level of cellulase production using cellulase-repressive glucose as a sole carbon source, along with intracellular acid accumulation and growth retardation. Our results indicated that intracellular acid accumulation in Δ76238 stimulated a significant increase in the cytosolic Ca2+ levels. Ca2+ channels were shown to be necessary for cellulase production using glucose as the carbon source in Δ76238. Delayed Δ76238 growth could be reversed by optimizing the medium's nitrogen sources to produce ammonia for intracellular acid neutralization in the early phase. This may be useful for scale-up of cellulase production using glucose as the carbon source.

Conclusions

This study provides a new perspective for significant alterations in the cellulase expression pattern of T. reesei Δ76238, indicating a new mechanism for cellulase regulation under conditions of low intracellular pH.

Authors+Show Affiliations

State Key Lab of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, P.O.B. 311, 130 Meilong Road, Shanghai, 200237 China.State Key Lab of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, P.O.B. 311, 130 Meilong Road, Shanghai, 200237 China.State Key Lab of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, P.O.B. 311, 130 Meilong Road, Shanghai, 200237 China.State Key Lab of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, P.O.B. 311, 130 Meilong Road, Shanghai, 200237 China.State Key Lab of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, P.O.B. 311, 130 Meilong Road, Shanghai, 200237 China.State Key Lab of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, P.O.B. 311, 130 Meilong Road, Shanghai, 200237 China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31417630

Citation

Liu, Pei, et al. "Enhanced Cellulase Production By Decreasing Intercellular pH Through H+-ATPase Gene Deletion in Trichoderma Reesei RUT-C30." Biotechnology for Biofuels, vol. 12, 2019, p. 195.
Liu P, Zhang G, Chen Y, et al. Enhanced cellulase production by decreasing intercellular pH through H+-ATPase gene deletion in Trichoderma reesei RUT-C30. Biotechnol Biofuels. 2019;12:195.
Liu, P., Zhang, G., Chen, Y., Zhao, J., Wang, W., & Wei, D. (2019). Enhanced cellulase production by decreasing intercellular pH through H+-ATPase gene deletion in Trichoderma reesei RUT-C30. Biotechnology for Biofuels, 12, p. 195. doi:10.1186/s13068-019-1536-2.
Liu P, et al. Enhanced Cellulase Production By Decreasing Intercellular pH Through H+-ATPase Gene Deletion in Trichoderma Reesei RUT-C30. Biotechnol Biofuels. 2019;12:195. PubMed PMID: 31417630.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enhanced cellulase production by decreasing intercellular pH through H+-ATPase gene deletion in Trichoderma reesei RUT-C30. AU - Liu,Pei, AU - Zhang,Guoxiu, AU - Chen,Yumeng, AU - Zhao,Jian, AU - Wang,Wei, AU - Wei,Dongzhi, Y1 - 2019/08/13/ PY - 2019/05/07/received PY - 2019/08/03/accepted PY - 2019/8/17/entrez PY - 2019/8/17/pubmed PY - 2019/8/17/medline KW - Ca2+ channels KW - Calcium signaling KW - Glucose KW - H+-ATPase KW - Intercellular pH KW - Trichoderma reesei SP - 195 EP - 195 JF - Biotechnology for biofuels JO - Biotechnol Biofuels VL - 12 N2 - Background: Cellulolytic enzymes produced by Trichoderma reesei are widely used for the industrial production of biofuels and chemicals from lignocellulose. We speculated that intracellular pH during the fermentation process can affect cellulase induction. Results: In this study, two H+-ATPase genes, tre76238 and tre78757, were first identified in T. reesei. Deletion of tre76238 and tre78757 in T. reesei RUT-C30 confirmed that tre76238 has a major function in maintaining intracellular pH, whereas tre78757 has a minor function. The tre76238 deletion strain Δ76238 displayed a high level of cellulase production using cellulase-repressive glucose as a sole carbon source, along with intracellular acid accumulation and growth retardation. Our results indicated that intracellular acid accumulation in Δ76238 stimulated a significant increase in the cytosolic Ca2+ levels. Ca2+ channels were shown to be necessary for cellulase production using glucose as the carbon source in Δ76238. Delayed Δ76238 growth could be reversed by optimizing the medium's nitrogen sources to produce ammonia for intracellular acid neutralization in the early phase. This may be useful for scale-up of cellulase production using glucose as the carbon source. Conclusions: This study provides a new perspective for significant alterations in the cellulase expression pattern of T. reesei Δ76238, indicating a new mechanism for cellulase regulation under conditions of low intracellular pH. SN - 1754-6834 UR - https://www.unboundmedicine.com/medline/citation/31417630/Enhanced_cellulase_production_by_decreasing_intercellular_pH_through_H+-ATPase_gene_deletion_in_Trichoderma_reesei_RUT-C30 L2 - https://biotechnologyforbiofuels.biomedcentral.com/articles/10.1186/s13068-019-1536-2 DB - PRIME DP - Unbound Medicine ER -