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Evaluation of the antioxidant effects of acid hydrolysates from Auricularia auricular polysaccharides using a Caenorhabditis elegans model.
Food Funct. 2019 Sep 01; 10(9):5531-5543.FF

Abstract

Caenorhabditis elegans is an important model organism for studying stress response mechanisms and identifying genetic pathways that influence longevity. The present study was designed to explore the in vivo-antioxidant potential and the probable mechanism of acid hydrolysates prepared from A. auricula polysaccharides (AAPHs-F) with the optimal acid hydrolysis conditions using Box-Behnken design, and C. elegans was used as a model organism. The effects of AAPHs-F on the locomotory behavior, lifespan, activities of antioxidant-related enzymes and levels of antioxidants in C. elegans were studied. In addition, the potential of AAPHs-F in up-regulating the expression of antioxidant-related genes in C. elegans, such as daf-16, skn-1, sod-1, sod-2 and sir-2.1, and the inhibition of cell apoptosis of C. elegans were also discussed. The results indicated that AAPHs-F could significantly increase the U-Turn frequency of nematodes, extend their lifespan, enhance antioxidant systems including superoxide dismutase (SOD) by 70.60%, catalase (CAT) by 73.45% and glutathione reductase (GR) by 258.68% (p < 0.01), increase the level of glutathione (GSH) by 110.22% (p < 0.01), and decrease the level of reactive oxygen species (ROS) and malondialdehyde (MDA) by 31.86% and 46.16% (p < 0.01), respectively. Quantitative real-time polymerase chain reaction (qRT-PCR) results showed that AAPHs-F could up-regulate mRNA expression levels of daf-16, skn-1, sir, sod-1 and sod-2 in wild-type C. elegans (>1.3 fold) when treated at a concentration of 0.1 mg mL-1 (p < 0.05 or p < 0.01). AAPHs-F was concluded to be heteropolysaccharides composed of mannose, glucose and galactose with a molar ratio of 12.7 : 3.25 : 1. The molecular weight of AAPHs-F was determined to be 885.37 Da. Furthermore, AAPHs-F is mainly formed of (1 → 3)-linked-α-d-glucopyranose, and carboxyl or acetamide is present in the molecule. In summary, our studies provide evidence that AAPHs-F helps improve the antioxidant defense system, and up-regulation of stress and longevity related genes suggests the possible involvement of these genes in the prevention of stress damage in C. elegans.

Authors+Show Affiliations

College of Life Sciences, China Jiliang University, Hangzhou, Zhejiang 310018, China. yjzhang@vip.163.com.College of Life Sciences, China Jiliang University, Hangzhou, Zhejiang 310018, China. yjzhang@vip.163.com.College of Modern Science and Technology, China Jiliang University, Zhejiang Province, China.College of Life Sciences, China Jiliang University, Hangzhou, Zhejiang 310018, China. yjzhang@vip.163.com.College of Life Sciences, China Jiliang University, Hangzhou, Zhejiang 310018, China. yjzhang@vip.163.com.College of Life Sciences, China Jiliang University, Hangzhou, Zhejiang 310018, China. yjzhang@vip.163.com.College of Life Sciences, China Jiliang University, Hangzhou, Zhejiang 310018, China. yjzhang@vip.163.com.College of Life Sciences, China Jiliang University, Hangzhou, Zhejiang 310018, China. yjzhang@vip.163.com.College of Life Sciences, China Jiliang University, Hangzhou, Zhejiang 310018, China. yjzhang@vip.163.com.College of Life Sciences, China Jiliang University, Hangzhou, Zhejiang 310018, China. yjzhang@vip.163.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31418439

Citation

Fang, Zhiyu, et al. "Evaluation of the Antioxidant Effects of Acid Hydrolysates From Auricularia Auricular Polysaccharides Using a Caenorhabditis Elegans Model." Food & Function, vol. 10, no. 9, 2019, pp. 5531-5543.
Fang Z, Chen Y, Wang G, et al. Evaluation of the antioxidant effects of acid hydrolysates from Auricularia auricular polysaccharides using a Caenorhabditis elegans model. Food Funct. 2019;10(9):5531-5543.
Fang, Z., Chen, Y., Wang, G., Feng, T., Shen, M., Xiao, B., Gu, J., Wang, W., Li, J., & Zhang, Y. (2019). Evaluation of the antioxidant effects of acid hydrolysates from Auricularia auricular polysaccharides using a Caenorhabditis elegans model. Food & Function, 10(9), 5531-5543. https://doi.org/10.1039/c8fo02589d
Fang Z, et al. Evaluation of the Antioxidant Effects of Acid Hydrolysates From Auricularia Auricular Polysaccharides Using a Caenorhabditis Elegans Model. Food Funct. 2019 Sep 1;10(9):5531-5543. PubMed PMID: 31418439.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of the antioxidant effects of acid hydrolysates from Auricularia auricular polysaccharides using a Caenorhabditis elegans model. AU - Fang,Zhiyu, AU - Chen,Yutao, AU - Wang,Ge, AU - Feng,Tao, AU - Shen,Meng, AU - Xiao,Bin, AU - Gu,Jingyi, AU - Wang,Weimin, AU - Li,Jia, AU - Zhang,Yongjun, Y1 - 2019/08/16/ PY - 2019/8/17/pubmed PY - 2020/2/8/medline PY - 2019/8/17/entrez SP - 5531 EP - 5543 JF - Food & function JO - Food Funct VL - 10 IS - 9 N2 - Caenorhabditis elegans is an important model organism for studying stress response mechanisms and identifying genetic pathways that influence longevity. The present study was designed to explore the in vivo-antioxidant potential and the probable mechanism of acid hydrolysates prepared from A. auricula polysaccharides (AAPHs-F) with the optimal acid hydrolysis conditions using Box-Behnken design, and C. elegans was used as a model organism. The effects of AAPHs-F on the locomotory behavior, lifespan, activities of antioxidant-related enzymes and levels of antioxidants in C. elegans were studied. In addition, the potential of AAPHs-F in up-regulating the expression of antioxidant-related genes in C. elegans, such as daf-16, skn-1, sod-1, sod-2 and sir-2.1, and the inhibition of cell apoptosis of C. elegans were also discussed. The results indicated that AAPHs-F could significantly increase the U-Turn frequency of nematodes, extend their lifespan, enhance antioxidant systems including superoxide dismutase (SOD) by 70.60%, catalase (CAT) by 73.45% and glutathione reductase (GR) by 258.68% (p < 0.01), increase the level of glutathione (GSH) by 110.22% (p < 0.01), and decrease the level of reactive oxygen species (ROS) and malondialdehyde (MDA) by 31.86% and 46.16% (p < 0.01), respectively. Quantitative real-time polymerase chain reaction (qRT-PCR) results showed that AAPHs-F could up-regulate mRNA expression levels of daf-16, skn-1, sir, sod-1 and sod-2 in wild-type C. elegans (>1.3 fold) when treated at a concentration of 0.1 mg mL-1 (p < 0.05 or p < 0.01). AAPHs-F was concluded to be heteropolysaccharides composed of mannose, glucose and galactose with a molar ratio of 12.7 : 3.25 : 1. The molecular weight of AAPHs-F was determined to be 885.37 Da. Furthermore, AAPHs-F is mainly formed of (1 → 3)-linked-α-d-glucopyranose, and carboxyl or acetamide is present in the molecule. In summary, our studies provide evidence that AAPHs-F helps improve the antioxidant defense system, and up-regulation of stress and longevity related genes suggests the possible involvement of these genes in the prevention of stress damage in C. elegans. SN - 2042-650X UR - https://www.unboundmedicine.com/medline/citation/31418439/Evaluation_of_the_antioxidant_effects_of_acid_hydrolysates_from_Auricularia_auricular_polysaccharides_using_a_Caenorhabditis_elegans_model_ L2 - https://doi.org/10.1039/c8fo02589d DB - PRIME DP - Unbound Medicine ER -