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Headspace Solid Phase Microextraction Coupled to GC/MS for the Analysis of Volatiles of Honeys from Arid and Mediterranean Areas of Algeria.
Chem Biodivers. 2019 Oct; 16(10):e1900267.CB

Abstract

The volatile composition of seven honey samples collected from various regions of Algeria and feeding on different plants have been determined. The Headspace Solid-Phase MicroExtraction (HS-SPME) coupled with Gas Chromatography-Mass Spectrometry (GC/MS) was used to achieve the purpose. In this work, different parameters of the HS-SPME analytical method were investigated in order to reach maximal sensitivity, and thus to obtain maximum information about the volatile profile of Algerian honey. These parameters include saline medium, HS extraction temperature, and the nature of the fiber used. The results showed a great diversity in the chemical composition, in total 124 compounds from different chemical classes were identified, including compounds found for the first time in honey. The Ascending Hierarchical Classification (AHC) demonstrated the importance of choosing SPME extraction conditions to find volatile compounds, which could be as specific markers of the floral or geographical origin of honey, the latter was optimized in the parameter PDMS-55 °C.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Neggad A
University of Sciences and Technology Houari Boumediene, Faculty of Chemistry, Laboratory of Functional Organic Analysis, BP 32 El-Alia, 16111, Bab-Ezzouar, Algiers, Algeria.
Benkaci-Ali F
University of Sciences and Technology Houari Boumediene, Faculty of Chemistry, Laboratory of Functional Organic Analysis, BP 32 El-Alia, 16111, Bab-Ezzouar, Algiers, Algeria.
Alsafra Z
University of Liege, Laboratoire de Spectrometrie de Masse L.S.M, Allee du 6 Ao슩t, Bât B6c, 4000, Liege (Sart-Tilman), Belgium.
Eppe G
University of Liege, Laboratoire de Spectrometrie de Masse L.S.M, Allee du 6 Ao슩t, Bât B6c, 4000, Liege (Sart-Tilman), Belgium.

MeSH

AlgeriaGas Chromatography-Mass SpectrometryHoneySolid Phase MicroextractionVolatile Organic Compounds

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31419038

Citation

Neggad, Abdelhamid, et al. "Headspace Solid Phase Microextraction Coupled to GC/MS for the Analysis of Volatiles of Honeys From Arid and Mediterranean Areas of Algeria." Chemistry & Biodiversity, vol. 16, no. 10, 2019, pp. e1900267.
Neggad A, Benkaci-Ali F, Alsafra Z, et al. Headspace Solid Phase Microextraction Coupled to GC/MS for the Analysis of Volatiles of Honeys from Arid and Mediterranean Areas of Algeria. Chem Biodivers. 2019;16(10):e1900267.
Neggad, A., Benkaci-Ali, F., Alsafra, Z., & Eppe, G. (2019). Headspace Solid Phase Microextraction Coupled to GC/MS for the Analysis of Volatiles of Honeys from Arid and Mediterranean Areas of Algeria. Chemistry & Biodiversity, 16(10), e1900267. https://doi.org/10.1002/cbdv.201900267
Neggad A, et al. Headspace Solid Phase Microextraction Coupled to GC/MS for the Analysis of Volatiles of Honeys From Arid and Mediterranean Areas of Algeria. Chem Biodivers. 2019;16(10):e1900267. PubMed PMID: 31419038.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Headspace Solid Phase Microextraction Coupled to GC/MS for the Analysis of Volatiles of Honeys from Arid and Mediterranean Areas of Algeria. AU - Neggad,Abdelhamid, AU - Benkaci-Ali,Farid, AU - Alsafra,Zouheir, AU - Eppe,Gauthier, Y1 - 2019/09/18/ PY - 2019/05/16/received PY - 2019/08/15/accepted PY - 2019/8/17/pubmed PY - 2019/11/7/medline PY - 2019/8/17/entrez KW - AHC statistic study KW - HS-SPME/GC/MS KW - honeys SP - e1900267 EP - e1900267 JF - Chemistry & biodiversity JO - Chem Biodivers VL - 16 IS - 10 N2 - The volatile composition of seven honey samples collected from various regions of Algeria and feeding on different plants have been determined. The Headspace Solid-Phase MicroExtraction (HS-SPME) coupled with Gas Chromatography-Mass Spectrometry (GC/MS) was used to achieve the purpose. In this work, different parameters of the HS-SPME analytical method were investigated in order to reach maximal sensitivity, and thus to obtain maximum information about the volatile profile of Algerian honey. These parameters include saline medium, HS extraction temperature, and the nature of the fiber used. The results showed a great diversity in the chemical composition, in total 124 compounds from different chemical classes were identified, including compounds found for the first time in honey. The Ascending Hierarchical Classification (AHC) demonstrated the importance of choosing SPME extraction conditions to find volatile compounds, which could be as specific markers of the floral or geographical origin of honey, the latter was optimized in the parameter PDMS-55 °C. SN - 1612-1880 UR - https://www.unboundmedicine.com/medline/citation/31419038/Headspace_Solid_Phase_Microextraction_Coupled_to_GC/MS_for_the_Analysis_of_Volatiles_of_Honeys_from_Arid_and_Mediterranean_Areas_of_Algeria_ DB - PRIME DP - Unbound Medicine ER -