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Induction and kinetics of complement-fixing antibodies against Plasmodium vivax MSP3α and relationship with IgG subclasses and IgM.

Abstract

BACKGROUND

Complement-fixing antibodies are important mediators of protection against Plasmodium falciparum malaria. However, complement-fixing antibodies remain uncharacterised for P. vivax malaria. Plasmodium vivax merozoite Surface Protein-3α (PvMSP3α) is a target of acquired immunity and a potential vaccine candidate.

METHODS

Plasma from children and adults with P. vivax malaria in Sabah, Malaysia, were collected during acute infection, 7 and 28 days following drug treatment. Complement-fixing antibodies and IgM and IgG, targeting three distinctive regions of PvMSP3α were measured by ELISA.

RESULTS

Seroprevalence of complement-fixing antibodies was highest against PvMSP3α Central region (77.6%). IgG1, IgG3, and IgM were significantly correlated with C1q-fixation and both purified IgG and IgM were capable of mediating C1q-fixation to PvMSP3α. Complement-fixing antibody levels were similar between age groups, but IgM was predominant in children and IgG3 more prevalent in adults. Functional antibodies increased following acute infection to 7 days after treatment, however rapidly waned by day 28.

CONCLUSION

Our study demonstrates PvMSP3α antibodies acquired during P. vivax infection can mediate complement-fixation and shows the important influence of age in shaping these specific antibody responses. Further studies are warranted to understand the role of these functional antibodies in protective immunity against P. vivax malaria.

Authors+Show Affiliations

Menzies School of Health Research, Darwin, NT, Australia. Charles Darwin University, Darwin, NT, Australia.Research Centre for Infectious Diseases, School of Biological Sciences, University of Adelaide, Adelaide, Australia. Burnet Institute, Melbourne, VIC, Australia.Menzies School of Health Research, Darwin, NT, Australia. Infectious Diseases Society, Sabah-Menzies School of Health Research Clinical Research Unit, Queen Elizabeth Hospital, Sabah, Malaysia.Infectious Diseases Society, Sabah-Menzies School of Health Research Clinical Research Unit, Queen Elizabeth Hospital, Sabah, Malaysia. Jesselton Medical Centre, Kota Kinabalu, Sabah, Malaysia. Gleneagles Medical Centre, Kota Kinabalu, Sabah, Malaysia.Emory Vaccine Center, Yerkes National Primate Research Center, Emory University, Atlanta, GA, USA.Emory Vaccine Center, Yerkes National Primate Research Center, Emory University, Atlanta, GA, USA. Division of Infectious Diseases, Department of Medicine, Emory University, Atlanta, GA, USA.Burnet Institute, Melbourne, VIC, Australia. Centre for Epidemiology and Biostatistics, Melbourne School of Population and Global Health, University of Melbourne, Melbourne, VIC, Australia. Department of Infectious Diseases, Monash University, Melbourne, VIC, Australia. Department of Epidemiology and Preventive Medicine, Monash University, Melbourne, VIC, Australia.Menzies School of Health Research, Darwin, NT, Australia. Infectious Diseases Society, Sabah-Menzies School of Health Research Clinical Research Unit, Queen Elizabeth Hospital, Sabah, Malaysia.Burnet Institute, Melbourne, VIC, Australia. Department of Immunology and Pathology, Monash University, Melbourne, VIC, Australia. Department of Microbiology, Monash University, Clayton, VIC, Australia. Department of Medicine, University of Melbourne, Parkville, VIC, Australia.Menzies School of Health Research, Darwin, NT, Australia.Menzies School of Health Research, Darwin, NT, Australia. Burnet Institute, Melbourne, VIC, Australia. QIMR Berghofer Medical Research Institute, Brisbane, QLD, Australia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31419296

Citation

Oyong, D A., et al. "Induction and Kinetics of Complement-fixing Antibodies Against Plasmodium Vivax MSP3α and Relationship With IgG Subclasses and IgM." The Journal of Infectious Diseases, 2019.
Oyong DA, Wilson DW, Barber BE, et al. Induction and kinetics of complement-fixing antibodies against Plasmodium vivax MSP3α and relationship with IgG subclasses and IgM. J Infect Dis. 2019.
Oyong, D. A., Wilson, D. W., Barber, B. E., William, T., Jiang, J., Galinski, M. R., ... Boyle, M. J. (2019). Induction and kinetics of complement-fixing antibodies against Plasmodium vivax MSP3α and relationship with IgG subclasses and IgM. The Journal of Infectious Diseases, doi:10.1093/infdis/jiz407.
Oyong DA, et al. Induction and Kinetics of Complement-fixing Antibodies Against Plasmodium Vivax MSP3α and Relationship With IgG Subclasses and IgM. J Infect Dis. 2019 Aug 17; PubMed PMID: 31419296.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Induction and kinetics of complement-fixing antibodies against Plasmodium vivax MSP3α and relationship with IgG subclasses and IgM. AU - Oyong,D A, AU - Wilson,D W, AU - Barber,B E, AU - William,T, AU - Jiang,J, AU - Galinski,M R, AU - Fowkes,F J I, AU - Grigg,M J, AU - Beeson,J G, AU - Anstey,N M, AU - Boyle,M J, Y1 - 2019/08/17/ PY - 2019/06/25/received PY - 2019/8/17/entrez PY - 2019/8/17/pubmed PY - 2019/8/17/medline KW - Plasmodium vivax KW - Complement-fixing antibodies KW - PvMSP3α KW - malaria JF - The Journal of infectious diseases JO - J. Infect. Dis. N2 - BACKGROUND: Complement-fixing antibodies are important mediators of protection against Plasmodium falciparum malaria. However, complement-fixing antibodies remain uncharacterised for P. vivax malaria. Plasmodium vivax merozoite Surface Protein-3α (PvMSP3α) is a target of acquired immunity and a potential vaccine candidate. METHODS: Plasma from children and adults with P. vivax malaria in Sabah, Malaysia, were collected during acute infection, 7 and 28 days following drug treatment. Complement-fixing antibodies and IgM and IgG, targeting three distinctive regions of PvMSP3α were measured by ELISA. RESULTS: Seroprevalence of complement-fixing antibodies was highest against PvMSP3α Central region (77.6%). IgG1, IgG3, and IgM were significantly correlated with C1q-fixation and both purified IgG and IgM were capable of mediating C1q-fixation to PvMSP3α. Complement-fixing antibody levels were similar between age groups, but IgM was predominant in children and IgG3 more prevalent in adults. Functional antibodies increased following acute infection to 7 days after treatment, however rapidly waned by day 28. CONCLUSION: Our study demonstrates PvMSP3α antibodies acquired during P. vivax infection can mediate complement-fixation and shows the important influence of age in shaping these specific antibody responses. Further studies are warranted to understand the role of these functional antibodies in protective immunity against P. vivax malaria. SN - 1537-6613 UR - https://www.unboundmedicine.com/medline/citation/31419296/Induction_and_kinetics_of_complement-fixing_antibodies_against_Plasmodium_vivax_MSP3α_and_relationship_with_IgG_subclasses_and_IgM L2 - https://academic.oup.com/jid/article-lookup/doi/10.1093/infdis/jiz407 DB - PRIME DP - Unbound Medicine ER -