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Transcriptomic analysis of clam extrapallial fluids reveals immunity and cytoskeleton alterations in the first week of Brown Ring Disease development.
Fish Shellfish Immunol 2019; 93:940-948FS

Abstract

The Brown Ring Disease is an infection caused by the bacterium Vibrio tapetis on the Manila clam Ruditapes philippinarum. The process of infection, in the extrapallial fluids (EPFs) of clams, involves alteration of immune functions, in particular on hemocytes which are the cells responsible of phagocytosis. Disorganization of the actin-cytoskeleton in infected clams is a part of what leads to this alteration. This study is the first transcriptomic approach based on collection of extrapallial fluids on living animals experimentally infected by V. tapetis. We performed differential gene expression analysis of EPFs in two experimental treatments (healthy-against infected-clams by V. tapetis), and showed the deregulation of 135 genes. In infected clams, a downregulation of transcripts implied in immune functions (lysosomal activity and complement- and lectin-dependent PRR pathways) was observed during infection. We also showed a deregulation of transcripts encoding proteins involved in the actin cytoskeleton organization such as an overexpression of β12-Thymosin (which is an actin sequestration protein) or a downregulation of proteins that closely interact with capping proteins such as Coactosin, that counteract action of capping proteins, or Profilin. We validated these transcriptomic results by cellular physiological analyses that showed a decrease of the lysosome amounts and the disorganization of actin cytoskeleton in infected hemocytes.

Authors+Show Affiliations

Univ Brest, CNRS, IRD, Ifremer, UMR 6539 LEMAR, F-29280, Plouzane, France. Electronic address: alexandra.rahmani@univ-brest.fr.Sorbonne Universités, Université Pierre et Marie Curie-Paris 6, CNRS, FR2424, Station Biologique de Roscoff, Roscoff, France.Univ Brest, CNRS, IRD, Ifremer, UMR 6539 LEMAR, F-29280, Plouzane, France.Univ Brest, CNRS, IRD, Ifremer, UMR 6539 LEMAR, F-29280, Plouzane, France.Univ Brest, CNRS, IRD, Ifremer, UMR 6539 LEMAR, F-29280, Plouzane, France.Centro de Ciências da Saúde, Instituto de Biologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.Centro de Ciências da Saúde, Instituto de Biologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.Centro de Ciências da Saúde, Instituto de Biologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.Univ Brest, CNRS, IRD, Ifremer, UMR 6539 LEMAR, F-29280, Plouzane, France. Electronic address: vianney.pichereau@univ-brest.fr.Univ Brest, CNRS, IRD, Ifremer, UMR 6539 LEMAR, F-29280, Plouzane, France. Electronic address: christine.paillard@univ-brest.fr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31419531

Citation

Rahmani, Alexandra, et al. "Transcriptomic Analysis of Clam Extrapallial Fluids Reveals Immunity and Cytoskeleton Alterations in the First Week of Brown Ring Disease Development." Fish & Shellfish Immunology, vol. 93, 2019, pp. 940-948.
Rahmani A, Corre E, Richard G, et al. Transcriptomic analysis of clam extrapallial fluids reveals immunity and cytoskeleton alterations in the first week of Brown Ring Disease development. Fish Shellfish Immunol. 2019;93:940-948.
Rahmani, A., Corre, E., Richard, G., Bidault, A., Lambert, C., Oliveira, L., ... Paillard, C. (2019). Transcriptomic analysis of clam extrapallial fluids reveals immunity and cytoskeleton alterations in the first week of Brown Ring Disease development. Fish & Shellfish Immunology, 93, pp. 940-948. doi:10.1016/j.fsi.2019.08.025.
Rahmani A, et al. Transcriptomic Analysis of Clam Extrapallial Fluids Reveals Immunity and Cytoskeleton Alterations in the First Week of Brown Ring Disease Development. Fish Shellfish Immunol. 2019 Aug 13;93:940-948. PubMed PMID: 31419531.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transcriptomic analysis of clam extrapallial fluids reveals immunity and cytoskeleton alterations in the first week of Brown Ring Disease development. AU - Rahmani,Alexandra, AU - Corre,Erwan, AU - Richard,Gaëlle, AU - Bidault,Adeline, AU - Lambert,Christophe, AU - Oliveira,Louisi, AU - Thompson,Cristiane, AU - Thompson,Fabiano, AU - Pichereau,Vianney, AU - Paillard,Christine, Y1 - 2019/08/13/ PY - 2019/02/21/received PY - 2019/08/09/revised PY - 2019/08/10/accepted PY - 2019/8/17/pubmed PY - 2019/8/17/medline PY - 2019/8/17/entrez KW - Actin cytoskeleton KW - Brown ring disease KW - Coactosin KW - Hemocytes KW - R. philippinarum KW - Resting cells KW - V. tapetis KW - β-Thymosin SP - 940 EP - 948 JF - Fish & shellfish immunology JO - Fish Shellfish Immunol. VL - 93 N2 - The Brown Ring Disease is an infection caused by the bacterium Vibrio tapetis on the Manila clam Ruditapes philippinarum. The process of infection, in the extrapallial fluids (EPFs) of clams, involves alteration of immune functions, in particular on hemocytes which are the cells responsible of phagocytosis. Disorganization of the actin-cytoskeleton in infected clams is a part of what leads to this alteration. This study is the first transcriptomic approach based on collection of extrapallial fluids on living animals experimentally infected by V. tapetis. We performed differential gene expression analysis of EPFs in two experimental treatments (healthy-against infected-clams by V. tapetis), and showed the deregulation of 135 genes. In infected clams, a downregulation of transcripts implied in immune functions (lysosomal activity and complement- and lectin-dependent PRR pathways) was observed during infection. We also showed a deregulation of transcripts encoding proteins involved in the actin cytoskeleton organization such as an overexpression of β12-Thymosin (which is an actin sequestration protein) or a downregulation of proteins that closely interact with capping proteins such as Coactosin, that counteract action of capping proteins, or Profilin. We validated these transcriptomic results by cellular physiological analyses that showed a decrease of the lysosome amounts and the disorganization of actin cytoskeleton in infected hemocytes. SN - 1095-9947 UR - https://www.unboundmedicine.com/medline/citation/31419531/Transcriptomic_analysis_of_clam_extra_pallial_fluids_reveals_immunity_and_cytoskeleton_alterations_in_the_first_week_of_Brown_Ring_Disease_development L2 - https://linkinghub.elsevier.com/retrieve/pii/S1050-4648(19)30825-3 DB - PRIME DP - Unbound Medicine ER -