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Curcumin C3 complex®/Bioperine® has antineoplastic activity in mesothelioma: an in vitro and in vivo analysis.
J Exp Clin Cancer Res 2019; 38(1):360JE

Abstract

BACKGROUND

A major limitation in the treatment for malignant mesothelioma is related to serious side effects caused by chemotherapeutics and to the development of cancer-resistance. Advances in cancer therapies have been reached thanks to the introduction of alternative approaches, such as the use of phytochemicals. Curcumin-C3complex®/Bioperine® is a commercially standardized extract containing a ratio-defined mixture of three curcuminoids and piperine that greatly increase its bioavailability. Interestingly, the anticancer effect of this formulation has been described in different studies and several clinical trials have been started, but to our knowledge none refers to human mesothelioma.

METHODS

Curcumin-C3complex®/Bioperine® anticancer effect was evaluated in vitro in different human mesothelioma cell lines analysing cell proliferation, colony-forming assay, wound healing assays, invasion assay and FACS analysis. In vivo anticancer properties were analysed in a mesothelioma xenograft mouse model in CD1 Nude mice.

RESULTS

Curcumin-C3complex®/Bioperine® in vitro induced growth inhibition in all mesothelioma cell lines analysed in a dose- and time-depended manner and reduced self-renewal cell migration and cell invasive ability. Cell death was due to apoptosis. The analysis of the molecular signalling pathway suggested that intrinsic apoptotic pathway is activated by this treatment. This treatment in vivo delayed the growth of the ectopic tumours in a mesothelioma xenograft mouse model.

CONCLUSIONS

Curcumin-C3complex®/Bioperine® treatment strongly reduces in vitro tumorigenic properties of mesothelioma cells by impairing cellular self-renewal ability, proliferative cell rate and cell migration and delays tumor growth in xenograft mouse model by reducing angiogenesis and increasing apoptosis. Considering that curcumin in vivo synergizes drug effects, its administration to treatment regimen may help to enhance drug therapeutic efficacy in mesothelioma. Our results suggest that implementation of standard pharmacological therapies with novel compounds may pave the way to develop alternative approaches to mesothelioma.

Authors+Show Affiliations

Institute of Biosciences and BioResources-UOS Naples CNR, via P. Castellino, 111-, 80131, Naples, Italy. Department of Biology, University of Naples Federico II, Complesso Universitario Monte Sant'Angelo via Cinthia, Naples, Italy.Institute of Biosciences and BioResources-UOS Naples CNR, via P. Castellino, 111-, 80131, Naples, Italy. IRCCS Neuromed, Localitá Camerelle, Pozzilli, IS, Italy.IRCCS Neuromed, Localitá Camerelle, Pozzilli, IS, Italy.Institute of Biosciences and BioResources-UOS Naples CNR, via P. Castellino, 111-, 80131, Naples, Italy.IRCCS Neuromed, Localitá Camerelle, Pozzilli, IS, Italy.IRCCS Neuromed, Localitá Camerelle, Pozzilli, IS, Italy.Institute of Biosciences and BioResources-UOS Naples CNR, via P. Castellino, 111-, 80131, Naples, Italy. alfonsobaldi@tiscali.it. Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania "L. Vanvitelli", Caserta, Italy. alfonsobaldi@tiscali.it.Institute of Biosciences and BioResources-UOS Naples CNR, via P. Castellino, 111-, 80131, Naples, Italy. stefania.crispi@ibbr.cnr.it.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31419989

Citation

Di Meo, Francesco, et al. "Curcumin C3 complex®/Bioperine® Has Antineoplastic Activity in Mesothelioma: an in Vitro and in Vivo Analysis." Journal of Experimental & Clinical Cancer Research : CR, vol. 38, no. 1, 2019, p. 360.
Di Meo F, Filosa S, Madonna M, et al. Curcumin C3 complex®/Bioperine® has antineoplastic activity in mesothelioma: an in vitro and in vivo analysis. J Exp Clin Cancer Res. 2019;38(1):360.
Di Meo, F., Filosa, S., Madonna, M., Giello, G., Di Pardo, A., Maglione, V., ... Crispi, S. (2019). Curcumin C3 complex®/Bioperine® has antineoplastic activity in mesothelioma: an in vitro and in vivo analysis. Journal of Experimental & Clinical Cancer Research : CR, 38(1), p. 360. doi:10.1186/s13046-019-1368-8.
Di Meo F, et al. Curcumin C3 complex®/Bioperine® Has Antineoplastic Activity in Mesothelioma: an in Vitro and in Vivo Analysis. J Exp Clin Cancer Res. 2019 Aug 16;38(1):360. PubMed PMID: 31419989.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Curcumin C3 complex®/Bioperine® has antineoplastic activity in mesothelioma: an in vitro and in vivo analysis. AU - Di Meo,Francesco, AU - Filosa,Stefania, AU - Madonna,Michele, AU - Giello,Gerarda, AU - Di Pardo,Alba, AU - Maglione,Vittorio, AU - Baldi,Alfonso, AU - Crispi,Stefania, Y1 - 2019/08/16/ PY - 2019/05/30/received PY - 2019/08/08/accepted PY - 2019/8/18/entrez PY - 2019/8/20/pubmed PY - 2019/8/20/medline KW - Curcumin C3 complex KW - Intrinsic apoptosis KW - Mesothelioma KW - Tumor growth inhibition SP - 360 EP - 360 JF - Journal of experimental & clinical cancer research : CR JO - J. Exp. Clin. Cancer Res. VL - 38 IS - 1 N2 - BACKGROUND: A major limitation in the treatment for malignant mesothelioma is related to serious side effects caused by chemotherapeutics and to the development of cancer-resistance. Advances in cancer therapies have been reached thanks to the introduction of alternative approaches, such as the use of phytochemicals. Curcumin-C3complex®/Bioperine® is a commercially standardized extract containing a ratio-defined mixture of three curcuminoids and piperine that greatly increase its bioavailability. Interestingly, the anticancer effect of this formulation has been described in different studies and several clinical trials have been started, but to our knowledge none refers to human mesothelioma. METHODS: Curcumin-C3complex®/Bioperine® anticancer effect was evaluated in vitro in different human mesothelioma cell lines analysing cell proliferation, colony-forming assay, wound healing assays, invasion assay and FACS analysis. In vivo anticancer properties were analysed in a mesothelioma xenograft mouse model in CD1 Nude mice. RESULTS: Curcumin-C3complex®/Bioperine® in vitro induced growth inhibition in all mesothelioma cell lines analysed in a dose- and time-depended manner and reduced self-renewal cell migration and cell invasive ability. Cell death was due to apoptosis. The analysis of the molecular signalling pathway suggested that intrinsic apoptotic pathway is activated by this treatment. This treatment in vivo delayed the growth of the ectopic tumours in a mesothelioma xenograft mouse model. CONCLUSIONS: Curcumin-C3complex®/Bioperine® treatment strongly reduces in vitro tumorigenic properties of mesothelioma cells by impairing cellular self-renewal ability, proliferative cell rate and cell migration and delays tumor growth in xenograft mouse model by reducing angiogenesis and increasing apoptosis. Considering that curcumin in vivo synergizes drug effects, its administration to treatment regimen may help to enhance drug therapeutic efficacy in mesothelioma. Our results suggest that implementation of standard pharmacological therapies with novel compounds may pave the way to develop alternative approaches to mesothelioma. SN - 1756-9966 UR - https://www.unboundmedicine.com/medline/citation/31419989/Curcumin_C3_complex®/Bioperine®_has_antineoplastic_activity_in_mesothelioma:_an_in_vitro_and_in_vivo_analysis L2 - https://jeccr.biomedcentral.com/articles/10.1186/s13046-019-1368-8 DB - PRIME DP - Unbound Medicine ER -