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Effects of Paenibacillus xylanexedens on growth performance, intestinal histomorphology, intestinal microflora, and immune response in broiler chickens challenged with Escherichia coli K88.
Poult Sci 2019PS

Abstract

This study investigated the effects of dietary Paenibacillus xylanexedens ysm1 supplementation on growth performance, intestinal morphology, immune response, and cecal microbiota of broiler chickens challenged with Escherichia coli K88. A total of 320 one-day-old male broiler chicks were randomly allocated to 4 treatments (8 floor pens, 10 birds/pen) including 1) negative control (NC) birds fed a basal diet and not challenged with E. coli K88; 2) positive control (PC) birds fed a basal diet and challenged with of E. coli K88; 3) P. xylanexedens ysm1 treatment (PRO) birds fed a basal diet supplemented with 1 × 109P. xylanexedens ysm1 cfu/kg feed and challenged with E. coli K88; and 4) antibiotic treatment (ANT) birds fed a basal diet supplemented with 20 mg of colistin sulphate/kg of feed and challenged with E. coli K88. The E. coli challenge decreased (P < 0.05) BWG in PC birds compared with the ANT birds on days 21 and 28. The FCR was higher (P < 0.01) in PC birds compared with the NC, PRO, and ANT birds on days 14, 21, and 28. Compared with the NC, PRO, and ANT birds on day 28, PC birds had shorter villi and higher number of goblet cells in both jejunum and ileum (P < 0.001). Irrespective of the dietary treatments, the E. coli challenge reduced the number of PCNA-positive cells in both the jejunum and ileum on day 28. Paenibacillus xylanexedens ysm1 treatment resulted in higher concentration of mucosal sIgA in the jejunum as compared to the other treatment groups on days 14 and 28. The numbers of cecal E. coli were reduced (P = 0.017) in broilers treated with P. xylanexedens ysm1 or antibiotic in comparison with the PC group on day 28. In conclusion, the present study demonstrated that dietary supplementation of this new probiotic bacteria P. xylanexedens ysm1 improved broiler performance by modulating intestinal morphology, enhancing immune response, and reducing the number of E. coli in the cecum.

Authors+Show Affiliations

Life Sciences Research and Application Centre, Gazi University, 06830 Ankara, Turkey.Department of Animal Nutrition & Nutritional Diseases, Faculty of Veterinary Medicine, Ankara University, 06110 Ankara, Turkey.Department of Histology Embryology, Faculty of Veterinary Medicine, Ankara University, 06110 Ankara, Turkey.Department of Animal Nutrition & Nutritional Diseases, Faculty of Veterinary Medicine, Ankara University, 06110 Ankara, Turkey.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31420669

Citation

Ekim, Burcu, et al. "Effects of Paenibacillus Xylanexedens On Growth Performance, Intestinal Histomorphology, Intestinal Microflora, and Immune Response in Broiler Chickens Challenged With Escherichia Coli K88." Poultry Science, 2019.
Ekim B, Calik A, Ceylan A, et al. Effects of Paenibacillus xylanexedens on growth performance, intestinal histomorphology, intestinal microflora, and immune response in broiler chickens challenged with Escherichia coli K88. Poult Sci. 2019.
Ekim, B., Calik, A., Ceylan, A., & Saçaklı, P. (2019). Effects of Paenibacillus xylanexedens on growth performance, intestinal histomorphology, intestinal microflora, and immune response in broiler chickens challenged with Escherichia coli K88. Poultry Science, doi:10.3382/ps/pez460.
Ekim B, et al. Effects of Paenibacillus Xylanexedens On Growth Performance, Intestinal Histomorphology, Intestinal Microflora, and Immune Response in Broiler Chickens Challenged With Escherichia Coli K88. Poult Sci. 2019 Aug 16; PubMed PMID: 31420669.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of Paenibacillus xylanexedens on growth performance, intestinal histomorphology, intestinal microflora, and immune response in broiler chickens challenged with Escherichia coli K88. AU - Ekim,Burcu, AU - Calik,Ali, AU - Ceylan,Ahmet, AU - Saçaklı,Pınar, Y1 - 2019/08/16/ PY - 2019/03/15/received PY - 2019/07/26/accepted PY - 2019/8/18/entrez KW - Escherichia coli K88 KW - Paenibacillus xylanexedens ysm1 KW - broiler KW - intestinal histomorphology KW - probiotic JF - Poultry science JO - Poult. Sci. N2 - This study investigated the effects of dietary Paenibacillus xylanexedens ysm1 supplementation on growth performance, intestinal morphology, immune response, and cecal microbiota of broiler chickens challenged with Escherichia coli K88. A total of 320 one-day-old male broiler chicks were randomly allocated to 4 treatments (8 floor pens, 10 birds/pen) including 1) negative control (NC) birds fed a basal diet and not challenged with E. coli K88; 2) positive control (PC) birds fed a basal diet and challenged with of E. coli K88; 3) P. xylanexedens ysm1 treatment (PRO) birds fed a basal diet supplemented with 1 × 109P. xylanexedens ysm1 cfu/kg feed and challenged with E. coli K88; and 4) antibiotic treatment (ANT) birds fed a basal diet supplemented with 20 mg of colistin sulphate/kg of feed and challenged with E. coli K88. The E. coli challenge decreased (P < 0.05) BWG in PC birds compared with the ANT birds on days 21 and 28. The FCR was higher (P < 0.01) in PC birds compared with the NC, PRO, and ANT birds on days 14, 21, and 28. Compared with the NC, PRO, and ANT birds on day 28, PC birds had shorter villi and higher number of goblet cells in both jejunum and ileum (P < 0.001). Irrespective of the dietary treatments, the E. coli challenge reduced the number of PCNA-positive cells in both the jejunum and ileum on day 28. Paenibacillus xylanexedens ysm1 treatment resulted in higher concentration of mucosal sIgA in the jejunum as compared to the other treatment groups on days 14 and 28. The numbers of cecal E. coli were reduced (P = 0.017) in broilers treated with P. xylanexedens ysm1 or antibiotic in comparison with the PC group on day 28. In conclusion, the present study demonstrated that dietary supplementation of this new probiotic bacteria P. xylanexedens ysm1 improved broiler performance by modulating intestinal morphology, enhancing immune response, and reducing the number of E. coli in the cecum. SN - 1525-3171 UR - https://www.unboundmedicine.com/medline/citation/31420669/Effects_of_Paenibacillus_xylanexedens_on_growth_performance,_intestinal_histomorphology,_intestinal_microflora,_and_immune_response_in_broiler_chickens_challenged_with_Escherichia_coli_K88 L2 - https://academic.oup.com/ps/article-lookup/doi/10.3382/ps/pez460 DB - PRIME DP - Unbound Medicine ER -