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Hsa_circ_101882 promotes migration and invasion of gastric cancer cells by regulating EMT.
J Clin Lab Anal 2019; :e23002JC

Abstract

BACKGROUND

At present, gastric cancer (GC) is a serious threat to human life and health. Non-coding circular RNAs (circRNAs) have been found abnormal expression in multiple tumors. However, circRNAs remain largely unknown in tumor progression. In the present study, we mainly examined the expression, function, and molecular mechanisms of a new circRNAs (hsa_circ_101882) in GC.

MATERIALS AND METHODS

The expression of hsa_circ_101882 in GC tissue, corresponding adjacent normal tissues, and GC cell lines was examined by RT-PCR. The function of hsa_circ_101882 in GC was evaluated by MTT assay, cell migration, and invasion assay, colony formation assay, and flow cytometric assay. The effect of hsa_circ_101882 on epithelial-to-mesenchymal transition (EMT)-related gene expression was detected by RT-PCR and Western blot.

RESULTS

Hsa_circ_101882 expression levels were significantly increased in GC tissue and GC cell lines. Functionally, low expression of hsa_circ_101882 revealed anti-tumor effects via inhibiting cell growth, migration, and invasion and promoting cell apoptosis. Mechanically, the dysregulated expression of hsa_circ_101882 affects EMT signaling pathway, which was examined by detecting E-cadherin, N-cadherin, vimentin, and Snail expression levels.

CONCLUSIONS

Therefore, our research reveals that hsa_circ_101882 is considered a metastasis promoter by activating EMT and may serve as a critical oncogene and potential new biomarker in GC.

Authors+Show Affiliations

Intensive-care Unit of Linyi Central Hospital, Linyi, China.Galactophore Department of Linyi Central Hospital, Linyi, China.Galactophore Department of Linyi Central Hospital, Linyi, China.Ultrasonic Department of Linyi Central Hospital, Linyi, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31420917

Citation

Yin, Gui-Hua, et al. "Hsa_circ_101882 Promotes Migration and Invasion of Gastric Cancer Cells By Regulating EMT." Journal of Clinical Laboratory Analysis, 2019, pp. e23002.
Yin GH, Gao FC, Tian J, et al. Hsa_circ_101882 promotes migration and invasion of gastric cancer cells by regulating EMT. J Clin Lab Anal. 2019.
Yin, G. H., Gao, F. C., Tian, J., & Zhang, W. B. (2019). Hsa_circ_101882 promotes migration and invasion of gastric cancer cells by regulating EMT. Journal of Clinical Laboratory Analysis, pp. e23002. doi:10.1002/jcla.23002.
Yin GH, et al. Hsa_circ_101882 Promotes Migration and Invasion of Gastric Cancer Cells By Regulating EMT. J Clin Lab Anal. 2019 Aug 16;e23002. PubMed PMID: 31420917.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hsa_circ_101882 promotes migration and invasion of gastric cancer cells by regulating EMT. AU - Yin,Gui-Hua, AU - Gao,Fu-Cun, AU - Tian,Juan, AU - Zhang,Wen-Bo, Y1 - 2019/08/16/ PY - 2019/06/20/received PY - 2019/07/18/revised PY - 2019/07/19/accepted PY - 2019/8/18/entrez KW - epithelial-to-mesenchymal transition KW - gastric cancer KW - hsa_circ_101882 KW - invasion KW - migration SP - e23002 EP - e23002 JF - Journal of clinical laboratory analysis JO - J. Clin. Lab. Anal. N2 - BACKGROUND: At present, gastric cancer (GC) is a serious threat to human life and health. Non-coding circular RNAs (circRNAs) have been found abnormal expression in multiple tumors. However, circRNAs remain largely unknown in tumor progression. In the present study, we mainly examined the expression, function, and molecular mechanisms of a new circRNAs (hsa_circ_101882) in GC. MATERIALS AND METHODS: The expression of hsa_circ_101882 in GC tissue, corresponding adjacent normal tissues, and GC cell lines was examined by RT-PCR. The function of hsa_circ_101882 in GC was evaluated by MTT assay, cell migration, and invasion assay, colony formation assay, and flow cytometric assay. The effect of hsa_circ_101882 on epithelial-to-mesenchymal transition (EMT)-related gene expression was detected by RT-PCR and Western blot. RESULTS: Hsa_circ_101882 expression levels were significantly increased in GC tissue and GC cell lines. Functionally, low expression of hsa_circ_101882 revealed anti-tumor effects via inhibiting cell growth, migration, and invasion and promoting cell apoptosis. Mechanically, the dysregulated expression of hsa_circ_101882 affects EMT signaling pathway, which was examined by detecting E-cadherin, N-cadherin, vimentin, and Snail expression levels. CONCLUSIONS: Therefore, our research reveals that hsa_circ_101882 is considered a metastasis promoter by activating EMT and may serve as a critical oncogene and potential new biomarker in GC. SN - 1098-2825 UR - https://www.unboundmedicine.com/medline/citation/31420917/Hsa_circ_101882_promotes_migration_and_invasion_of_gastric_cancer_cells_by_regulating_EMT L2 - https://doi.org/10.1002/jcla.23002 DB - PRIME DP - Unbound Medicine ER -