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MAT1 facilitates the lung metastasis of osteosarcoma through upregulation of AKT1 expression.
Life Sci 2019; 234:116771LS

Abstract

AIMS

We aimed to elucidate the effects and mechanisms of MAT1 in the progression of osteosarcoma, especially for its lung metastasis.

MAIN METHODS

CCK-8 and flow cytometry assays were carried out to detect the proliferation and apoptosis of osteosarcoma cells. Wound healing and transwell assays were used to determine cell migration and invasion abilities. Real time quantitative PCR (RT-PCR) and western blot technologies were applied to detect the expression levels of RNA and protein, respectively.

KEY FINDS

The results showed that both the mRNA and protein expression levels of MAT1 were elevated in osteosarcoma tissues with lung metastasis and metastatic lung tissues, particularly in the metastatic lung tissues, as compared to the osteosarcoma tissues without lung metastasis. High expression level of MAT1 in osteosarcoma patients showed a negative association with the overall survival. In addition, upregulation of MAT1 induced significant increases in cell growth, migration and invasion and an obvious inhibition in cell apoptosis in osteosarcoma MG63 and 143B cells, as well as elevated AKT1 expression level. Moreover, knockdown of AKT1 obviously impaired MAT1-mediated promotions in cell migration and invasion in vitro, as well as repressed tumor growth and reduced the number of metastatic lung tumors in xenografted nude mice.

SIGNIFICANCE

This study reveals that high expression of MAT1 closely related to the poor prognosis and malignant clinical process of osteosarcoma patients. MAT1 serves as a promoter in the lung metastasis of osteosarcoma through increasing AKT1 expression. Our study may provide a potent therapeutic target for the lung metastasis of osteosarcoma.

Authors+Show Affiliations

Department of Orthopaedics, The 2nd Xiangya Hospital, Central South University, Changsha 410011, China.Department of Orthopaedics, The 2nd Xiangya Hospital, Central South University, Changsha 410011, China. Electronic address: nijiangdong001@csu.edu.cn.Department of Orthopaedics, The 2nd Xiangya Hospital, Central South University, Changsha 410011, China.Department of Orthopaedics, The 2nd Xiangya Hospital, Central South University, Changsha 410011, China.Department of Orthopaedics, The 2nd Xiangya Hospital, Central South University, Changsha 410011, China.Department of Orthopaedics, The 2nd Xiangya Hospital, Central South University, Changsha 410011, China.Department of Orthopaedics, The 2nd Xiangya Hospital, Central South University, Changsha 410011, China.

Pub Type(s)

Journal Article
Review

Language

eng

PubMed ID

31421084

Citation

Wang, Junjie, et al. "MAT1 Facilitates the Lung Metastasis of Osteosarcoma Through Upregulation of AKT1 Expression." Life Sciences, vol. 234, 2019, p. 116771.
Wang J, Ni J, Song D, et al. MAT1 facilitates the lung metastasis of osteosarcoma through upregulation of AKT1 expression. Life Sci. 2019;234:116771.
Wang, J., Ni, J., Song, D., Ding, M., Huang, J., Li, W., & He, G. (2019). MAT1 facilitates the lung metastasis of osteosarcoma through upregulation of AKT1 expression. Life Sciences, 234, p. 116771. doi:10.1016/j.lfs.2019.116771.
Wang J, et al. MAT1 Facilitates the Lung Metastasis of Osteosarcoma Through Upregulation of AKT1 Expression. Life Sci. 2019 Oct 1;234:116771. PubMed PMID: 31421084.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - MAT1 facilitates the lung metastasis of osteosarcoma through upregulation of AKT1 expression. AU - Wang,Junjie, AU - Ni,Jiangdong, AU - Song,Deye, AU - Ding,Muliang, AU - Huang,Jun, AU - Li,Wenzhao, AU - He,Guangxu, Y1 - 2019/08/14/ PY - 2019/03/29/received PY - 2019/08/05/revised PY - 2019/08/13/accepted PY - 2019/8/20/pubmed PY - 2019/8/20/medline PY - 2019/8/18/entrez KW - AKT1 KW - Lung metastasis KW - MAT1 KW - Osteosarcoma SP - 116771 EP - 116771 JF - Life sciences JO - Life Sci. VL - 234 N2 - AIMS: We aimed to elucidate the effects and mechanisms of MAT1 in the progression of osteosarcoma, especially for its lung metastasis. MAIN METHODS: CCK-8 and flow cytometry assays were carried out to detect the proliferation and apoptosis of osteosarcoma cells. Wound healing and transwell assays were used to determine cell migration and invasion abilities. Real time quantitative PCR (RT-PCR) and western blot technologies were applied to detect the expression levels of RNA and protein, respectively. KEY FINDS: The results showed that both the mRNA and protein expression levels of MAT1 were elevated in osteosarcoma tissues with lung metastasis and metastatic lung tissues, particularly in the metastatic lung tissues, as compared to the osteosarcoma tissues without lung metastasis. High expression level of MAT1 in osteosarcoma patients showed a negative association with the overall survival. In addition, upregulation of MAT1 induced significant increases in cell growth, migration and invasion and an obvious inhibition in cell apoptosis in osteosarcoma MG63 and 143B cells, as well as elevated AKT1 expression level. Moreover, knockdown of AKT1 obviously impaired MAT1-mediated promotions in cell migration and invasion in vitro, as well as repressed tumor growth and reduced the number of metastatic lung tumors in xenografted nude mice. SIGNIFICANCE: This study reveals that high expression of MAT1 closely related to the poor prognosis and malignant clinical process of osteosarcoma patients. MAT1 serves as a promoter in the lung metastasis of osteosarcoma through increasing AKT1 expression. Our study may provide a potent therapeutic target for the lung metastasis of osteosarcoma. SN - 1879-0631 UR - https://www.unboundmedicine.com/medline/citation/31421084/MAT1_facilitates_the_lung_metastasis_of_osteosarcoma_through_upregulation_of_AKT1_expression L2 - https://linkinghub.elsevier.com/retrieve/pii/S0024-3205(19)30698-8 DB - PRIME DP - Unbound Medicine ER -