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Rapid and automated production of [68Ga]gallium chloride and [68Ga]Ga-DOTA-TATE on a medical cyclotron.
Nucl Med Biol 2019; 74-75:12-18NM

Abstract

INTRODUCTION

The demand for Gallium-68 (68Ga) for labelling PET radiopharmaceuticals has increased over the past few years. 68Ga is obtained through the decayed parent radionuclide 68Ge using commercial 68Ge/68Ga generators. The principal limitation of commercial 68Ge/68Ga generators is that only a limited and finite quantity of 68Ga (<1.85 GBq at start of synthesis) may be accessed. The focus of this study was to investigate the use of a low energy medical cyclotron for the production of greater quantities of 68Ga and to develop an automated and rapid procedure for processing the product.

METHODS

Enriched ZnCl2 was electrodeposited on a platinum backing using a NH4Cl (pH 2-4) buffer. The Zn target was irradiated with GE PETtrace 880 at 35 μA and 14.5 and 12.0 MeV beam energy. The irradiated Zn target was purified using octanol resin on an automated system.

RESULTS

Following the described procedure, 68Ga was obtained in 6.30 ± 0.42 GBq after 8.5 min bombardment and with low radionuclidic impurities (66Ga (<0.005%) and 67Ga (<0.09%)). Purification on a single octanol resin gave 82% recovery with resulting [68Ga]GaCl3 obtained in 3.5 mL of 0.2 M HCl. [68Ga]GaCl3 production from irradiation to final product was <45 min. To highlight the utility of the automated procedure, [68Ga]Ga-DOTA-TATE labelling was incorporated to give 1.56 GBq at EOS of the labelled peptide with RCY of >70%.

CONCLUSIONS

A straightforward procedure for producing 68Ga on a low energy medical cyclotron was described. Current efforts are focus on high activity production and radiolabelling using solid target produced 68Ga.

Authors+Show Affiliations

Molecular Imaging and Therapy Research Unit, South Australian Health and Medical Research Institute, Adelaide, Australia. Electronic address: William.Tieu@SAHMRI.com.Molecular Imaging and Therapy Research Unit, South Australian Health and Medical Research Institute, Adelaide, Australia.Molecular Imaging and Therapy Research Unit, South Australian Health and Medical Research Institute, Adelaide, Australia.Molecular Imaging and Therapy Research Unit, South Australian Health and Medical Research Institute, Adelaide, Australia.School of Physical Sciences, Institute for Photonics and Advanced Sensing (IPAS), and Institute for Mineral and Energy Resources (IMER), University of Adelaide, North Terrace Campus, Adelaide, SA 5005, Australia.School of Physical Sciences, Institute for Photonics and Advanced Sensing (IPAS), and Institute for Mineral and Energy Resources (IMER), University of Adelaide, North Terrace Campus, Adelaide, SA 5005, Australia; Weapons and Combat Systems Division, Defence Science and Technology Group, P.O. Box 1500, Edinburgh 5111, Australia.Comecer S.p.A., Via Maestri del Lavoro 90, Castel Bolognese, RA, Italy.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31421441

Citation

Tieu, William, et al. "Rapid and Automated Production of [68Ga]gallium Chloride and [68Ga]Ga-DOTA-TATE On a Medical Cyclotron." Nuclear Medicine and Biology, vol. 74-75, 2019, pp. 12-18.
Tieu W, Hollis CA, Kuan KKW, et al. Rapid and automated production of [68Ga]gallium chloride and [68Ga]Ga-DOTA-TATE on a medical cyclotron. Nucl Med Biol. 2019;74-75:12-18.
Tieu, W., Hollis, C. A., Kuan, K. K. W., Takhar, P., Stuckings, M., Spooner, N., & Malinconico, M. (2019). Rapid and automated production of [68Ga]gallium chloride and [68Ga]Ga-DOTA-TATE on a medical cyclotron. Nuclear Medicine and Biology, 74-75, pp. 12-18. doi:10.1016/j.nucmedbio.2019.07.005.
Tieu W, et al. Rapid and Automated Production of [68Ga]gallium Chloride and [68Ga]Ga-DOTA-TATE On a Medical Cyclotron. Nucl Med Biol. 2019 Jul 12;74-75:12-18. PubMed PMID: 31421441.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid and automated production of [68Ga]gallium chloride and [68Ga]Ga-DOTA-TATE on a medical cyclotron. AU - Tieu,William, AU - Hollis,Courtney A, AU - Kuan,Kevin K W, AU - Takhar,Prab, AU - Stuckings,Mick, AU - Spooner,Nigel, AU - Malinconico,Mario, Y1 - 2019/07/12/ PY - 2018/12/19/received PY - 2019/06/06/revised PY - 2019/07/11/accepted PY - 2019/8/20/pubmed PY - 2019/8/20/medline PY - 2019/8/18/entrez KW - Cyclotron KW - DOTA-TATE KW - Ga-68 KW - PET KW - Solid targetry SP - 12 EP - 18 JF - Nuclear medicine and biology JO - Nucl. Med. Biol. VL - 74-75 N2 - INTRODUCTION: The demand for Gallium-68 (68Ga) for labelling PET radiopharmaceuticals has increased over the past few years. 68Ga is obtained through the decayed parent radionuclide 68Ge using commercial 68Ge/68Ga generators. The principal limitation of commercial 68Ge/68Ga generators is that only a limited and finite quantity of 68Ga (<1.85 GBq at start of synthesis) may be accessed. The focus of this study was to investigate the use of a low energy medical cyclotron for the production of greater quantities of 68Ga and to develop an automated and rapid procedure for processing the product. METHODS: Enriched ZnCl2 was electrodeposited on a platinum backing using a NH4Cl (pH 2-4) buffer. The Zn target was irradiated with GE PETtrace 880 at 35 μA and 14.5 and 12.0 MeV beam energy. The irradiated Zn target was purified using octanol resin on an automated system. RESULTS: Following the described procedure, 68Ga was obtained in 6.30 ± 0.42 GBq after 8.5 min bombardment and with low radionuclidic impurities (66Ga (<0.005%) and 67Ga (<0.09%)). Purification on a single octanol resin gave 82% recovery with resulting [68Ga]GaCl3 obtained in 3.5 mL of 0.2 M HCl. [68Ga]GaCl3 production from irradiation to final product was <45 min. To highlight the utility of the automated procedure, [68Ga]Ga-DOTA-TATE labelling was incorporated to give 1.56 GBq at EOS of the labelled peptide with RCY of >70%. CONCLUSIONS: A straightforward procedure for producing 68Ga on a low energy medical cyclotron was described. Current efforts are focus on high activity production and radiolabelling using solid target produced 68Ga. SN - 1872-9614 UR - https://www.unboundmedicine.com/medline/citation/31421441/Rapid_and_automated_production_of_[68Ga]gallium_chloride_and_[68Ga]Ga-DOTA-TATE_on_a_medical_cyclotron L2 - https://linkinghub.elsevier.com/retrieve/pii/S0969-8051(18)30365-2 DB - PRIME DP - Unbound Medicine ER -