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Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) in bulk tank milk, livestock and dairy-farm personnel in north-central and north-eastern Greece: Prevalence, characterization and genetic relatedness.
Food Microbiol. 2019 Dec; 84:103249.FM

Abstract

Recently, there has been an increased tendency towards raw-milk consumption, which may pose a consumer risk, due to the possible presence of human pathogenic microorganisms, such as Staphylococcus aureus and even methicillin-resistant S. aureus (MRSA). The prevalence of S. aureus and methicillin-resistant S. aureus (MRSA) was investigated in 40 dairy (cattle, sheep and goat) farms in northern Greece. S. aureus and MRSA were detected in 47.8% and 4.1% of the 387 samples (raw milk, farmers and animal samples) tested, respectively. Most (81.3%) of the MRSA isolates harbored the mecA gene, whereas the mecC or Panton-Valentine Leucocidin (PVL) genes were not detected. Seven spa types were identified, with t127 being the most prevalent. Spa type t034 (CC398) was isolated for the first time from livestock in Greece. Staphylococcal enterotoxin genes were detected in 93.8% of the MRSA isolates. The MRSA isolates were genetically diverse and were all capable of biofilm production. Our results confirm the lurking threat of MRSA in raw milk and dairy farms and suggest the need for surveillance programs starting at the farm level.

Authors+Show Affiliations

Laboratory of Hygiene of Foods of Animal Origin-Veterinary Public Health, School of Veterinary Medicine, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece. Electronic address: ppanagie@vet.auth.gr.Laboratory of Safety and Quality of Milk and Dairy Products, School of Veterinary Medicine, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece.Department of Epidemiology and Public Health, Sciensano, 1050, Brussels, Belgium; Veterinary Research Institute of Thessaloniki, Greek Agricultural Organization-DIMITRA, 57006, Thermi, Greece.Veterinary Research Institute of Thessaloniki, Greek Agricultural Organization-DIMITRA, 57006, Thermi, Greece.Veterinary Research Institute of Thessaloniki, Greek Agricultural Organization-DIMITRA, 57006, Thermi, Greece.Department of Microbiology, Medical School, Aristotle University of Thessaloniki, 54 124, Thessaloniki, Greece.Laboratory of Microbiology and Infectious Diseases, School of Veterinary Medicine, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece.Laboratory of Hygiene of Foods of Animal Origin-Veterinary Public Health, School of Veterinary Medicine, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31421759

Citation

Papadopoulos, Panagiotis, et al. "Staphylococcus Aureus and Methicillin-resistant S. Aureus (MRSA) in Bulk Tank Milk, Livestock and Dairy-farm Personnel in North-central and North-eastern Greece: Prevalence, Characterization and Genetic Relatedness." Food Microbiology, vol. 84, 2019, p. 103249.
Papadopoulos P, Angelidis AS, Papadopoulos T, et al. Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) in bulk tank milk, livestock and dairy-farm personnel in north-central and north-eastern Greece: Prevalence, characterization and genetic relatedness. Food Microbiol. 2019;84:103249.
Papadopoulos, P., Angelidis, A. S., Papadopoulos, T., Kotzamanidis, C., Zdragas, A., Papa, A., Filioussis, G., & Sergelidis, D. (2019). Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) in bulk tank milk, livestock and dairy-farm personnel in north-central and north-eastern Greece: Prevalence, characterization and genetic relatedness. Food Microbiology, 84, 103249. https://doi.org/10.1016/j.fm.2019.103249
Papadopoulos P, et al. Staphylococcus Aureus and Methicillin-resistant S. Aureus (MRSA) in Bulk Tank Milk, Livestock and Dairy-farm Personnel in North-central and North-eastern Greece: Prevalence, Characterization and Genetic Relatedness. Food Microbiol. 2019;84:103249. PubMed PMID: 31421759.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) in bulk tank milk, livestock and dairy-farm personnel in north-central and north-eastern Greece: Prevalence, characterization and genetic relatedness. AU - Papadopoulos,Panagiotis, AU - Angelidis,Apostolos S, AU - Papadopoulos,Theofilos, AU - Kotzamanidis,Charalampos, AU - Zdragas,Antonios, AU - Papa,Anna, AU - Filioussis,George, AU - Sergelidis,Daniel, Y1 - 2019/06/22/ PY - 2018/11/15/received PY - 2019/06/21/revised PY - 2019/06/21/accepted PY - 2019/8/19/entrez PY - 2019/8/20/pubmed PY - 2019/12/18/medline KW - Biofilm KW - Enterotoxins KW - MRSA KW - PFGE KW - Raw milk KW - Spa typing SP - 103249 EP - 103249 JF - Food microbiology JO - Food Microbiol VL - 84 N2 - Recently, there has been an increased tendency towards raw-milk consumption, which may pose a consumer risk, due to the possible presence of human pathogenic microorganisms, such as Staphylococcus aureus and even methicillin-resistant S. aureus (MRSA). The prevalence of S. aureus and methicillin-resistant S. aureus (MRSA) was investigated in 40 dairy (cattle, sheep and goat) farms in northern Greece. S. aureus and MRSA were detected in 47.8% and 4.1% of the 387 samples (raw milk, farmers and animal samples) tested, respectively. Most (81.3%) of the MRSA isolates harbored the mecA gene, whereas the mecC or Panton-Valentine Leucocidin (PVL) genes were not detected. Seven spa types were identified, with t127 being the most prevalent. Spa type t034 (CC398) was isolated for the first time from livestock in Greece. Staphylococcal enterotoxin genes were detected in 93.8% of the MRSA isolates. The MRSA isolates were genetically diverse and were all capable of biofilm production. Our results confirm the lurking threat of MRSA in raw milk and dairy farms and suggest the need for surveillance programs starting at the farm level. SN - 1095-9998 UR - https://www.unboundmedicine.com/medline/citation/31421759/Staphylococcus_aureus_and_methicillin_resistant_S__aureus__MRSA__in_bulk_tank_milk_livestock_and_dairy_farm_personnel_in_north_central_and_north_eastern_Greece:_Prevalence_characterization_and_genetic_relatedness_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0740-0020(18)31085-2 DB - PRIME DP - Unbound Medicine ER -