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Element content and expression of genes of interest in guard cells are connected to spatiotemporal variations in stomatal conductance.

Abstract

Element content and expression of genes of interest on single cell types, such as stomata, provide valuable insights into their specific physiology, improving our understanding of leaf gas exchange regulation. We investigated how far differences in stomatal conductance (gs) can be ascribed to changes in guard cells functioning in amphistomateous leaves. gs was measured during the day on both leaf sides, on well-watered and drought-stressed trees (two Populus euramericana Moench and two Populus nigra L. genotypes). In parallel, guard cells were dissected for element content and gene expressions analyses. Both were strongly arranged according to genotype, and drought had the lowest impact overall. Normalizing the data by genotype highlighted a structure on the basis of leaf sides and time of day both for element content and gene expression. Guard cells magnesium, phosphorus, and chlorine were the most abundant on the abaxial side in the morning, where gs was at the highest. In contrast, genes encoding H+ -ATPase and aquaporins were usually more abundant in the afternoon, whereas genes encoding Ca2+ -vacuolar antiporters, K+ channels, and ABA-related genes were in general more abundant on the adaxial side. Our work highlights the unique physiology of each leaf side and their analogous rhythmicity through the day.

Authors+Show Affiliations

Inra, Université de Lorraine, AgroParisTech, SILVA, F-54280, Champenoux, France.Inra, Université de Lorraine, AgroParisTech, SILVA, F-54280, Champenoux, France.Inra, Université de Lorraine, AgroParisTech, SILVA, F-54280, Champenoux, France.Inra, Université de Lorraine, AgroParisTech, SILVA, F-54280, Champenoux, France.Department of Genetics and Physiology of Forest Trees, Faculty of Forestry and Wood Sciences, Czech University of Life Sciences Prague, Prague, 165 00, Czech Republic.Inra, Université de Lorraine, AgroParisTech, SILVA, F-54280, Champenoux, France.Inra, Université de Lorraine, AgroParisTech, SILVA, F-54280, Champenoux, France.Inra, Université de Lorraine, AgroParisTech, SILVA, F-54280, Champenoux, France.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31423592

Citation

Durand, Maxime, et al. "Element Content and Expression of Genes of Interest in Guard Cells Are Connected to Spatiotemporal Variations in Stomatal Conductance." Plant, Cell & Environment, 2019.
Durand M, Cohen D, Aubry N, et al. Element content and expression of genes of interest in guard cells are connected to spatiotemporal variations in stomatal conductance. Plant Cell Environ. 2019.
Durand, M., Cohen, D., Aubry, N., Buré, C., Tomášková, I., Hummel, I., ... Le Thiec, D. (2019). Element content and expression of genes of interest in guard cells are connected to spatiotemporal variations in stomatal conductance. Plant, Cell & Environment, doi:10.1111/pce.13644.
Durand M, et al. Element Content and Expression of Genes of Interest in Guard Cells Are Connected to Spatiotemporal Variations in Stomatal Conductance. Plant Cell Environ. 2019 Aug 18; PubMed PMID: 31423592.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Element content and expression of genes of interest in guard cells are connected to spatiotemporal variations in stomatal conductance. AU - Durand,Maxime, AU - Cohen,David, AU - Aubry,Nathalie, AU - Buré,Cyril, AU - Tomášková,Ivana, AU - Hummel,Irène, AU - Brendel,Oliver, AU - Le Thiec,Didier, Y1 - 2019/08/18/ PY - 2019/04/24/received PY - 2019/08/11/accepted PY - 2019/8/20/pubmed PY - 2019/8/20/medline PY - 2019/8/20/entrez KW - Populus KW - abaxial and adaxial surfaces KW - droughts KW - elements KW - gene expression KW - plant stomata KW - stomatal conductance JF - Plant, cell & environment JO - Plant Cell Environ. N2 - Element content and expression of genes of interest on single cell types, such as stomata, provide valuable insights into their specific physiology, improving our understanding of leaf gas exchange regulation. We investigated how far differences in stomatal conductance (gs) can be ascribed to changes in guard cells functioning in amphistomateous leaves. gs was measured during the day on both leaf sides, on well-watered and drought-stressed trees (two Populus euramericana Moench and two Populus nigra L. genotypes). In parallel, guard cells were dissected for element content and gene expressions analyses. Both were strongly arranged according to genotype, and drought had the lowest impact overall. Normalizing the data by genotype highlighted a structure on the basis of leaf sides and time of day both for element content and gene expression. Guard cells magnesium, phosphorus, and chlorine were the most abundant on the abaxial side in the morning, where gs was at the highest. In contrast, genes encoding H+ -ATPase and aquaporins were usually more abundant in the afternoon, whereas genes encoding Ca2+ -vacuolar antiporters, K+ channels, and ABA-related genes were in general more abundant on the adaxial side. Our work highlights the unique physiology of each leaf side and their analogous rhythmicity through the day. SN - 1365-3040 UR - https://www.unboundmedicine.com/medline/citation/31423592/Element_content_and_expression_of_genes_of_interest_in_guard_cells_are_connected_to_spatiotemporal_variations_in_stomatal_conductance L2 - https://doi.org/10.1111/pce.13644 DB - PRIME DP - Unbound Medicine ER -