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Low Methoxyl Pectin Protects against Autoimmune Diabetes and Associated Caecal Dysfunction.
Mol Nutr Food Res 2019; :e1900307MN

Abstract

SCOPE

This study aims to examine the protective effects of specific low-methoxyl pectin (LMP) on the development of type 1 diabetes (T1D).

METHODS AND RESULTS

Female non-obese diabetic (NOD) mice are weaned onto either control or 5% LMP supplemented diets for up to 22 weeks of age. T1D incidence, gut barrier function, and pancreatic-gut immune responses are analyzed. LMP supplementation significantly dampened the onset of T1D in NOD mice. LMP supplementation induces caecal homeostasis, as indicated by the increasing SCFAs production, higher expression of tight junction proteins claudin 1, zonula occludens-2 in caecum. Furthermore, LMP-mediated caecal homeostasis impacts gut-pancreatic immunity, as evidenced by increased regulatory T cell population, modulated inflammatory cytokine expression, and suppressed NOD like receptor protein 3 (NLRP3) inflammasome activation in both caecum and pancreas.

CONCLUSION

The data demonstrate that LMP limits T1D development by inducing caecal homeostasis to shape pancreatic immune environment, providing a scientific basis for using LMP as a novel functional supplementation to intervene T1D.

Authors+Show Affiliations

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, P. R. China. School of Food Science and Technology, Jiangnan University, Wuxi, 214122, P. R. China.School of Medicine, Jiangnan University, Wuxi, 214122, P. R. China.State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, P. R. China. School of Food Science and Technology, Jiangnan University, Wuxi, 214122, P. R. China.State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, P. R. China. School of Food Science and Technology, Jiangnan University, Wuxi, 214122, P. R. China.State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, P. R. China. School of Food Science and Technology, Jiangnan University, Wuxi, 214122, P. R. China.State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, P. R. China. School of Food Science and Technology, Jiangnan University, Wuxi, 214122, P. R. China.State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, P. R. China. School of Food Science and Technology, Jiangnan University, Wuxi, 214122, P. R. China.State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, P. R. China. School of Food Science and Technology, Jiangnan University, Wuxi, 214122, P. R. China.State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, P. R. China. School of Food Science and Technology, Jiangnan University, Wuxi, 214122, P. R. China.College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, P. R. China.State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, P. R. China. School of Food Science and Technology, Jiangnan University, Wuxi, 214122, P. R. China.State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, P. R. China. School of Food Science and Technology, Jiangnan University, Wuxi, 214122, P. R. China.Department of Surgery, University of California Irvine, Orange, CA, 92868, USA.Division of Clinical Microbiology, Department of Laboratory Medicine, Karolinska Institute, Karolinska University Hospital, 17177, Stockholm, Sweden.Division of Medical Biology, Department of Pathology and Medical Biology, University of Groningen, University Medical Center Groningen, 9713 GZ, Groningen, The Netherlands.State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, P. R. China. School of Food Science and Technology, Jiangnan University, Wuxi, 214122, P. R. China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31423661

Citation

Wu, Chengfei, et al. "Low Methoxyl Pectin Protects Against Autoimmune Diabetes and Associated Caecal Dysfunction." Molecular Nutrition & Food Research, 2019, pp. e1900307.
Wu C, Pan LL, Luo Y, et al. Low Methoxyl Pectin Protects against Autoimmune Diabetes and Associated Caecal Dysfunction. Mol Nutr Food Res. 2019.
Wu, C., Pan, L. L., Luo, Y., Niu, W., Fang, X., Liang, W., ... Sun, J. (2019). Low Methoxyl Pectin Protects against Autoimmune Diabetes and Associated Caecal Dysfunction. Molecular Nutrition & Food Research, pp. e1900307. doi:10.1002/mnfr.201900307.
Wu C, et al. Low Methoxyl Pectin Protects Against Autoimmune Diabetes and Associated Caecal Dysfunction. Mol Nutr Food Res. 2019 Aug 18;e1900307. PubMed PMID: 31423661.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Low Methoxyl Pectin Protects against Autoimmune Diabetes and Associated Caecal Dysfunction. AU - Wu,Chengfei, AU - Pan,Li-Long, AU - Luo,Yang, AU - Niu,Wenying, AU - Fang,Xin, AU - Liang,Wenjie, AU - Li,Jiahong, AU - Li,Hongli, AU - Pan,Xiaohua, AU - Yang,Guilian, AU - Chen,Wei, AU - Zhang,Hao, AU - Lakey,Jonathan R T, AU - Agerberth,Birgitta, AU - de Vos,Paul, AU - Sun,Jia, Y1 - 2019/08/18/ PY - 2019/04/08/received PY - 2019/07/29/revised PY - 2019/8/20/pubmed PY - 2019/8/20/medline PY - 2019/8/20/entrez KW - autoimmune diabetes KW - caecal homeostasis KW - immunomodulation KW - low-methoxyl pectin SP - e1900307 EP - e1900307 JF - Molecular nutrition & food research JO - Mol Nutr Food Res N2 - SCOPE: This study aims to examine the protective effects of specific low-methoxyl pectin (LMP) on the development of type 1 diabetes (T1D). METHODS AND RESULTS: Female non-obese diabetic (NOD) mice are weaned onto either control or 5% LMP supplemented diets for up to 22 weeks of age. T1D incidence, gut barrier function, and pancreatic-gut immune responses are analyzed. LMP supplementation significantly dampened the onset of T1D in NOD mice. LMP supplementation induces caecal homeostasis, as indicated by the increasing SCFAs production, higher expression of tight junction proteins claudin 1, zonula occludens-2 in caecum. Furthermore, LMP-mediated caecal homeostasis impacts gut-pancreatic immunity, as evidenced by increased regulatory T cell population, modulated inflammatory cytokine expression, and suppressed NOD like receptor protein 3 (NLRP3) inflammasome activation in both caecum and pancreas. CONCLUSION: The data demonstrate that LMP limits T1D development by inducing caecal homeostasis to shape pancreatic immune environment, providing a scientific basis for using LMP as a novel functional supplementation to intervene T1D. SN - 1613-4133 UR - https://www.unboundmedicine.com/medline/citation/31423661/Low_Methoxyl_Pectin_Protects_Against_Autoimmune_Diabetes_and_Associated_Caecal_Dysfunction L2 - https://doi.org/10.1002/mnfr.201900307 DB - PRIME DP - Unbound Medicine ER -