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Sensitive and Specific Detection of Low-Level Antibody Responses in Mild Middle East Respiratory Syndrome Coronavirus Infections.
Emerg Infect Dis. 2019 10; 25(10):1868-1877.EI

Abstract

Middle East respiratory syndrome coronavirus (MERS-CoV) infections in humans can cause asymptomatic to fatal lower respiratory lung disease. Despite posing a probable risk for virus transmission, asymptomatic to mild infections can go unnoticed; a lack of seroconversion among some PCR-confirmed cases has been reported. We found that a MERS-CoV spike S1 protein-based ELISA, routinely used in surveillance studies, showed low sensitivity in detecting infections among PCR-confirmed patients with mild clinical symptoms and cross-reactivity of human coronavirus OC43-positive serum samples. Using in-house S1 ELISA and protein microarray, we demonstrate that most PCR-confirmed MERS-CoV case-patients with mild infections seroconverted; nonetheless, some of these samples did not have detectable levels of virus-neutralizing antibodies. The use of a sensitive and specific serologic S1-based assay can be instrumental in the accurate estimation of MERS-CoV prevalence.

Authors

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Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31423970

Citation

Okba, Nisreen M A., et al. "Sensitive and Specific Detection of Low-Level Antibody Responses in Mild Middle East Respiratory Syndrome Coronavirus Infections." Emerging Infectious Diseases, vol. 25, no. 10, 2019, pp. 1868-1877.
Okba NMA, Raj VS, Widjaja I, et al. Sensitive and Specific Detection of Low-Level Antibody Responses in Mild Middle East Respiratory Syndrome Coronavirus Infections. Emerg Infect Dis. 2019;25(10):1868-1877.
Okba, N. M. A., Raj, V. S., Widjaja, I., GeurtsvanKessel, C. H., de Bruin, E., Chandler, F. D., Park, W. B., Kim, N. J., Farag, E. A. B. A., Al-Hajri, M., Bosch, B. J., Oh, M. D., Koopmans, M. P. G., Reusken, C. B. E. M., & Haagmans, B. L. (2019). Sensitive and Specific Detection of Low-Level Antibody Responses in Mild Middle East Respiratory Syndrome Coronavirus Infections. Emerging Infectious Diseases, 25(10), 1868-1877. https://doi.org/10.3201/eid2510.190051
Okba NMA, et al. Sensitive and Specific Detection of Low-Level Antibody Responses in Mild Middle East Respiratory Syndrome Coronavirus Infections. Emerg Infect Dis. 2019;25(10):1868-1877. PubMed PMID: 31423970.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sensitive and Specific Detection of Low-Level Antibody Responses in Mild Middle East Respiratory Syndrome Coronavirus Infections. AU - Okba,Nisreen M A, AU - Raj,V Stalin, AU - Widjaja,Ivy, AU - GeurtsvanKessel,Corine H, AU - de Bruin,Erwin, AU - Chandler,Felicity D, AU - Park,Wan Beom, AU - Kim,Nam-Joong, AU - Farag,Elmoubasher A B A, AU - Al-Hajri,Mohammed, AU - Bosch,Berend-Jan, AU - Oh,Myoung-Don, AU - Koopmans,Marion P G, AU - Reusken,Chantal B E M, AU - Haagmans,Bart L, Y1 - 2019/10/17/ PY - 2019/8/20/pubmed PY - 2020/7/15/medline PY - 2019/8/20/entrez KW - ELISA KW - MERS KW - MERS-CoV KW - Middle East respiratory syndrome coronavirus KW - Qatar KW - S1 KW - South Korea KW - antibodies KW - camels KW - coronavirus KW - diagnostics KW - human KW - neutralizing KW - serology KW - spike KW - the Netherlands KW - viruses SP - 1868 EP - 1877 JF - Emerging infectious diseases JO - Emerg Infect Dis VL - 25 IS - 10 N2 - Middle East respiratory syndrome coronavirus (MERS-CoV) infections in humans can cause asymptomatic to fatal lower respiratory lung disease. Despite posing a probable risk for virus transmission, asymptomatic to mild infections can go unnoticed; a lack of seroconversion among some PCR-confirmed cases has been reported. We found that a MERS-CoV spike S1 protein-based ELISA, routinely used in surveillance studies, showed low sensitivity in detecting infections among PCR-confirmed patients with mild clinical symptoms and cross-reactivity of human coronavirus OC43-positive serum samples. Using in-house S1 ELISA and protein microarray, we demonstrate that most PCR-confirmed MERS-CoV case-patients with mild infections seroconverted; nonetheless, some of these samples did not have detectable levels of virus-neutralizing antibodies. The use of a sensitive and specific serologic S1-based assay can be instrumental in the accurate estimation of MERS-CoV prevalence. SN - 1080-6059 UR - https://www.unboundmedicine.com/medline/citation/31423970/Sensitive_and_Specific_Detection_of_Low_Level_Antibody_Responses_in_Mild_Middle_East_Respiratory_Syndrome_Coronavirus_Infections_ L2 - https://doi.org/10.3201/eid2510.190051 DB - PRIME DP - Unbound Medicine ER -