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Protective effect and mechanism of alpha-lipoic acid on partial hepatic ischemia-reperfusion injury in adult male rats.
Physiol Res. 2019 10 25; 68(5):739-745.PR

Abstract

In order to reduce tissue damage caused by ischemia-reperfusion injury, this study aims to investigate the protective effect and mechanism of ?-lipoic acid on hepatic ischemia-reperfusion injury in rats. The bloodstream of rats was blocked in the left middle and left lateral liver lobes of the liver. Forty rats were randomly divided into two groups: treatment group and injury group. Rats were injected with either 25 mg/1 ml of alpha-lipoic acid (treatment group) or 1 ml of saline (injury group) into the caudal vein 15 min before hepatic ischemia-reperfusion. Rat serum alanine aminotransferase (GPT), glutathione (GSH) and superoxide dismutase (SOD) levels were examined at various time points (1, 3, 6 and 12 h) in both groups. Changes in nuclear factor kappa B P65 (NF-kappaB P65) expression in ischemia-reperfusion liver at various time points after reperfusion (1, 3, 6 and 12 h) were evaluated through immunohistochemistry assay. Changes in macrophage inflammatory protein-2 (MIP-2) mRNA and inducible nitric oxide synthase (iNOS) mRNA expression in ischemic reperfused rat livers were detected by RT-PCR. Serum GPT level was significantly higher in the injury group than in the treatment group (P<0.01). NF-kappaB P65, MIP-2 mRNA and iNOS mRNA expression in ischemic reperfused rat livers were significantly higher in the injury group than in the treatment group (P<0.01). Serum GSH and SOD levels were higher in the treatment group than in the injury group (P<0.01). Alpha-lipoic acid significantly reduced ischemia-reperfusion injury in rat livers. This may be associated to the direct scavenging of oxygen-free radicals, increased GSH production, and the activation of downstream media due to decreased NF-kappaB and GSH consumption.

Authors+Show Affiliations

The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China. renyun880923@163.com.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31424256

Citation

Ren, Y, et al. "Protective Effect and Mechanism of Alpha-lipoic Acid On Partial Hepatic Ischemia-reperfusion Injury in Adult Male Rats." Physiological Research, vol. 68, no. 5, 2019, pp. 739-745.
Ren Y, Wang LH, Deng FS, et al. Protective effect and mechanism of alpha-lipoic acid on partial hepatic ischemia-reperfusion injury in adult male rats. Physiol Res. 2019;68(5):739-745.
Ren, Y., Wang, L. H., Deng, F. S., Li, J. S., & Jiang, L. (2019). Protective effect and mechanism of alpha-lipoic acid on partial hepatic ischemia-reperfusion injury in adult male rats. Physiological Research, 68(5), 739-745.
Ren Y, et al. Protective Effect and Mechanism of Alpha-lipoic Acid On Partial Hepatic Ischemia-reperfusion Injury in Adult Male Rats. Physiol Res. 2019 10 25;68(5):739-745. PubMed PMID: 31424256.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effect and mechanism of alpha-lipoic acid on partial hepatic ischemia-reperfusion injury in adult male rats. AU - Ren,Y, AU - Wang,L-H, AU - Deng,F-S, AU - Li,J-S, AU - Jiang,L, Y1 - 2019/08/19/ PY - 2019/8/20/pubmed PY - 2020/4/23/medline PY - 2019/8/20/entrez SP - 739 EP - 745 JF - Physiological research JO - Physiol Res VL - 68 IS - 5 N2 - In order to reduce tissue damage caused by ischemia-reperfusion injury, this study aims to investigate the protective effect and mechanism of ?-lipoic acid on hepatic ischemia-reperfusion injury in rats. The bloodstream of rats was blocked in the left middle and left lateral liver lobes of the liver. Forty rats were randomly divided into two groups: treatment group and injury group. Rats were injected with either 25 mg/1 ml of alpha-lipoic acid (treatment group) or 1 ml of saline (injury group) into the caudal vein 15 min before hepatic ischemia-reperfusion. Rat serum alanine aminotransferase (GPT), glutathione (GSH) and superoxide dismutase (SOD) levels were examined at various time points (1, 3, 6 and 12 h) in both groups. Changes in nuclear factor kappa B P65 (NF-kappaB P65) expression in ischemia-reperfusion liver at various time points after reperfusion (1, 3, 6 and 12 h) were evaluated through immunohistochemistry assay. Changes in macrophage inflammatory protein-2 (MIP-2) mRNA and inducible nitric oxide synthase (iNOS) mRNA expression in ischemic reperfused rat livers were detected by RT-PCR. Serum GPT level was significantly higher in the injury group than in the treatment group (P<0.01). NF-kappaB P65, MIP-2 mRNA and iNOS mRNA expression in ischemic reperfused rat livers were significantly higher in the injury group than in the treatment group (P<0.01). Serum GSH and SOD levels were higher in the treatment group than in the injury group (P<0.01). Alpha-lipoic acid significantly reduced ischemia-reperfusion injury in rat livers. This may be associated to the direct scavenging of oxygen-free radicals, increased GSH production, and the activation of downstream media due to decreased NF-kappaB and GSH consumption. SN - 1802-9973 UR - https://www.unboundmedicine.com/medline/citation/31424256/Protective_effect_and_mechanism_of_alpha_lipoic_acid_on_partial_hepatic_ischemia_reperfusion_injury_in_adult_male_rats_ L2 - http://www.biomed.cas.cz/physiolres/pdf/68/68_739.pdf DB - PRIME DP - Unbound Medicine ER -