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Vaccination with recombinant Toxoplasma gondii CDPK3 induces protective immunity against experimental toxoplasmosis.
Acta Trop 2019; 199:105148AT

Abstract

Toxoplasma gondii, a ubiquitous and obligate intracellular pathogen, belonging to the phylum Apicomplexa, is capable of infecting a broad range of warm-blooded hosts including birds and mammals that is nearly worldwide. Preventive measures for toxoplasmosis are currently lacking and as such, development of novel vaccines is of urgent need. The plant-like calcium-dependent protein kinases (CDPKs) expressed by T. gondii, play important roles in cell invasion, gliding motility, egress and some other developmental processes, in which T. gondii CDPK3 (TgCDPK3) has been implicated as an important virulence factor. In this study, the immune protective function of recombinant TgCDPK3 (rTgCDPK3) against experimental toxoplasmosis in BALB/c were evaluated. We divided the mice into different dose groups of vaccines and all immunizations with purified rTgCDPK3 protein were injected by intramuscular at weeks 0, 2, and 4 in BALB/c mice. The rTgCDPK3 vaccine provided protection was correlated with the development of humoral and cellular immune responses demonstrated through the antigen-specific spleen cell proliferation, release of Th1 cytokines IFN-γ, and the production of the high titers of IgG antibody with a predominance of IgG2a over IgG1. Vaccination with rTgCDPK3 conferred partial protection against acute toxoplasmosis, as demonstrated by prolonged survival rate after lethal challenge. Additionally, the amount of brain tissues cysts in vaccinated mice led to 46.5% reduction compared with non-vaccinated ones. These data demonstrated that rTgCDPK3 inoculation prevents or attenuates the harmful influence of T. gondii infection, and it is a potential vaccine candidate against toxoplasmosis.

Authors+Show Affiliations

Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Anhui Medical University, Hefei 230032, China; Anhui Provincial Key Laboratory of Microbiology & Parasitology, Anhui Medical University, Hefei 230032, China.Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Anhui Medical University, Hefei 230032, China; Anhui Provincial Key Laboratory of Microbiology & Parasitology, Anhui Medical University, Hefei 230032, China.Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Anhui Medical University, Hefei 230032, China; Anhui Provincial Key Laboratory of Microbiology & Parasitology, Anhui Medical University, Hefei 230032, China; School of Nursing, Anhui Medical University, Hefei 230601, China.The Second Clinical Medical School, Hefei 230032, China.Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Anhui Medical University, Hefei 230032, China; Anhui Provincial Key Laboratory of Microbiology & Parasitology, Anhui Medical University, Hefei 230032, China.Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Anhui Medical University, Hefei 230032, China; Anhui Provincial Key Laboratory of Microbiology & Parasitology, Anhui Medical University, Hefei 230032, China.Anhui Provincial Key Laboratory of Microbiology & Parasitology, Anhui Medical University, Hefei 230032, China.Department of Anesthesiology, the First Affiliated Hospital of Anhui Medical University, No. 218 Jixi Road, Hefei 230032, China. Electronic address: chenlijian77@126.com.Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Anhui Medical University, Hefei 230032, China; Anhui Provincial Key Laboratory of Microbiology & Parasitology, Anhui Medical University, Hefei 230032, China. Electronic address: dujian@ahmu.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31425673

Citation

Wu, Minmin, et al. "Vaccination With Recombinant Toxoplasma Gondii CDPK3 Induces Protective Immunity Against Experimental Toxoplasmosis." Acta Tropica, vol. 199, 2019, p. 105148.
Wu M, An R, Chen Y, et al. Vaccination with recombinant Toxoplasma gondii CDPK3 induces protective immunity against experimental toxoplasmosis. Acta Trop. 2019;199:105148.
Wu, M., An, R., Chen, Y., Chen, T., Wen, H., Yan, Q., ... Du, J. (2019). Vaccination with recombinant Toxoplasma gondii CDPK3 induces protective immunity against experimental toxoplasmosis. Acta Tropica, 199, p. 105148. doi:10.1016/j.actatropica.2019.105148.
Wu M, et al. Vaccination With Recombinant Toxoplasma Gondii CDPK3 Induces Protective Immunity Against Experimental Toxoplasmosis. Acta Trop. 2019 Aug 16;199:105148. PubMed PMID: 31425673.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Vaccination with recombinant Toxoplasma gondii CDPK3 induces protective immunity against experimental toxoplasmosis. AU - Wu,Minmin, AU - An,Ran, AU - Chen,Ying, AU - Chen,Tianyang, AU - Wen,Hongyang, AU - Yan,Qi, AU - Shen,Jilong, AU - Chen,Lijian, AU - Du,Jian, Y1 - 2019/08/16/ PY - 2019/06/20/received PY - 2019/08/06/revised PY - 2019/08/14/accepted PY - 2019/8/20/pubmed PY - 2019/8/20/medline PY - 2019/8/20/entrez KW - Protective efficacy KW - Protein vaccine KW - T. gondii KW - TgCDPK3 KW - Toxoplasmosis SP - 105148 EP - 105148 JF - Acta tropica JO - Acta Trop. VL - 199 N2 - Toxoplasma gondii, a ubiquitous and obligate intracellular pathogen, belonging to the phylum Apicomplexa, is capable of infecting a broad range of warm-blooded hosts including birds and mammals that is nearly worldwide. Preventive measures for toxoplasmosis are currently lacking and as such, development of novel vaccines is of urgent need. The plant-like calcium-dependent protein kinases (CDPKs) expressed by T. gondii, play important roles in cell invasion, gliding motility, egress and some other developmental processes, in which T. gondii CDPK3 (TgCDPK3) has been implicated as an important virulence factor. In this study, the immune protective function of recombinant TgCDPK3 (rTgCDPK3) against experimental toxoplasmosis in BALB/c were evaluated. We divided the mice into different dose groups of vaccines and all immunizations with purified rTgCDPK3 protein were injected by intramuscular at weeks 0, 2, and 4 in BALB/c mice. The rTgCDPK3 vaccine provided protection was correlated with the development of humoral and cellular immune responses demonstrated through the antigen-specific spleen cell proliferation, release of Th1 cytokines IFN-γ, and the production of the high titers of IgG antibody with a predominance of IgG2a over IgG1. Vaccination with rTgCDPK3 conferred partial protection against acute toxoplasmosis, as demonstrated by prolonged survival rate after lethal challenge. Additionally, the amount of brain tissues cysts in vaccinated mice led to 46.5% reduction compared with non-vaccinated ones. These data demonstrated that rTgCDPK3 inoculation prevents or attenuates the harmful influence of T. gondii infection, and it is a potential vaccine candidate against toxoplasmosis. SN - 1873-6254 UR - https://www.unboundmedicine.com/medline/citation/31425673/Vaccination_with_Recombinant_Toxoplasma_gondii_CDPK3_induces_protective_immunity_against_experimental_toxoplasmosis L2 - https://linkinghub.elsevier.com/retrieve/pii/S0001-706X(19)30816-2 DB - PRIME DP - Unbound Medicine ER -