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Spatiotemporal Expression of Anticoagulation Factor Antistasin in Freshwater Leeches.
Int J Mol Sci 2019; 20(16)IJ

Abstract

Antistasin, which was originally discovered in the salivary glands of the Mexican leech Haementeria officinalis, was newly isolated from Helobdella austinensis. To confirm the temporal expression of antistasin during embryogenesis, we carried out semi-quantitative RT-PCR. Hau-antistasin1 was uniquely expressed at stage 4 of the cleavage and was strongly expressed in the late stages of organogenesis, as were other antistasin members. In order to confirm the spatial expression of antistasin, we performed fluorescence in situ hybridization in the late stages of organogenesis. The expression of each antistasin in the proboscis showed a similar pattern and varied in expression in the body. In addition, the spatial expression of antistasin orthologs in different leeches showed the possibility of different function across leech species. Hau-antistasin1 was expressed in the same region as hedgehog, which is a known mediator of signal transduction pathway. Hau-antistasin1 is probably a downstream target of Hedgehog signaling, involved in segment polarity signal pathway.

Authors+Show Affiliations

School of Biological Sciences, College of Natural Sciences, Chungbuk National University, Cheongju, Chungbuk 28644, Korea.School of Biological Sciences, College of Natural Sciences, Chungbuk National University, Cheongju, Chungbuk 28644, Korea. School of Life Sciences, Gwangju Institute of Science and Technology (GIST), Gwangju 61005, Korea.School of Biological Sciences, College of Natural Sciences, Chungbuk National University, Cheongju, Chungbuk 28644, Korea. Department of Earth Sciences, Paleobiology, Uppsala University, Villavägen 16, 75236 Uppsala, Sweden.Department of Life Sciences, Chung-Ang University, Seoul 156-756, Korea.School of Biological Sciences, College of Natural Sciences, Chungbuk National University, Cheongju, Chungbuk 28644, Korea. sjchobio@chungbuk.ac.kr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31426335

Citation

Kwak, Hee-Jin, et al. "Spatiotemporal Expression of Anticoagulation Factor Antistasin in Freshwater Leeches." International Journal of Molecular Sciences, vol. 20, no. 16, 2019.
Kwak HJ, Park JS, Medina Jiménez BI, et al. Spatiotemporal Expression of Anticoagulation Factor Antistasin in Freshwater Leeches. Int J Mol Sci. 2019;20(16).
Kwak, H. J., Park, J. S., Medina Jiménez, B. I., Park, S. C., & Cho, S. J. (2019). Spatiotemporal Expression of Anticoagulation Factor Antistasin in Freshwater Leeches. International Journal of Molecular Sciences, 20(16), doi:10.3390/ijms20163994.
Kwak HJ, et al. Spatiotemporal Expression of Anticoagulation Factor Antistasin in Freshwater Leeches. Int J Mol Sci. 2019 Aug 16;20(16) PubMed PMID: 31426335.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Spatiotemporal Expression of Anticoagulation Factor Antistasin in Freshwater Leeches. AU - Kwak,Hee-Jin, AU - Park,Jeong-Su, AU - Medina Jiménez,Brenda Irene, AU - Park,Soon Cheol, AU - Cho,Sung-Jin, Y1 - 2019/08/16/ PY - 2019/07/09/received PY - 2019/08/07/revised PY - 2019/08/14/accepted PY - 2019/8/21/entrez PY - 2019/8/21/pubmed PY - 2019/8/21/medline KW - Antistasin KW - Helobdella KW - expression KW - hedgehog signaling KW - in situ hybridization JF - International journal of molecular sciences JO - Int J Mol Sci VL - 20 IS - 16 N2 - Antistasin, which was originally discovered in the salivary glands of the Mexican leech Haementeria officinalis, was newly isolated from Helobdella austinensis. To confirm the temporal expression of antistasin during embryogenesis, we carried out semi-quantitative RT-PCR. Hau-antistasin1 was uniquely expressed at stage 4 of the cleavage and was strongly expressed in the late stages of organogenesis, as were other antistasin members. In order to confirm the spatial expression of antistasin, we performed fluorescence in situ hybridization in the late stages of organogenesis. The expression of each antistasin in the proboscis showed a similar pattern and varied in expression in the body. In addition, the spatial expression of antistasin orthologs in different leeches showed the possibility of different function across leech species. Hau-antistasin1 was expressed in the same region as hedgehog, which is a known mediator of signal transduction pathway. Hau-antistasin1 is probably a downstream target of Hedgehog signaling, involved in segment polarity signal pathway. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/31426335/Spatiotemporal_Expression_of_Anticoagulation_Factor_Antistasin_in_Freshwater_Leeches L2 - http://www.mdpi.com/resolver?pii=ijms20163994 DB - PRIME DP - Unbound Medicine ER -