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Inactivation of Aldehyde Dehydrogenase by Disulfiram in the Presence and Absence of Lipoic Acid or Dihydrolipoic Acid: An in Vitro Study.
Biomolecules 2019; 9(8)B

Abstract

The inhibition of aldehyde dehydrogenase (ALDH) by disulfiram (DSF) in vitro can be prevented and/or reversed by dithiothreitol (DTT), which is a well-known low molecular weight non-physiological redox reagent commonly used in laboratory experiments. These observations inspired us to ask the question whether the inhibition of ALDH by DSF can be preserved or abolished also by dihydrolipoic acid (DHLA), which is the only currently known low molecular weight physiological dithiol in the body of humans and other animals. It can even be metaphorized that DHLA is an "endogenous DTT". Lipoic acid (LA) is the oxidized form of DHLA. We investigated the inactivation of ALDH derived from yeast and rat liver by DSF in the presence or absence of LA or DHLA. The results clearly show that DHLA is able both to restore and protect ALDH activity blocked by DSF. The proposed mechanism is discussed.

Authors+Show Affiliations

Chair of Medical Biochemistry, Jagiellonian University Medical College, Kraków 31-034, Poland. mbbilska@cyf-kr.edu.pl.Chair of Medical Biochemistry, Jagiellonian University Medical College, Kraków 31-034, Poland.Chair of Medical Biochemistry, Jagiellonian University Medical College, Kraków 31-034, Poland.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31426424

Citation

Bilska-Wilkosz, Anna, et al. "Inactivation of Aldehyde Dehydrogenase By Disulfiram in the Presence and Absence of Lipoic Acid or Dihydrolipoic Acid: an in Vitro Study." Biomolecules, vol. 9, no. 8, 2019.
Bilska-Wilkosz A, Górny M, Iciek M. Inactivation of Aldehyde Dehydrogenase by Disulfiram in the Presence and Absence of Lipoic Acid or Dihydrolipoic Acid: An in Vitro Study. Biomolecules. 2019;9(8).
Bilska-Wilkosz, A., Górny, M., & Iciek, M. (2019). Inactivation of Aldehyde Dehydrogenase by Disulfiram in the Presence and Absence of Lipoic Acid or Dihydrolipoic Acid: An in Vitro Study. Biomolecules, 9(8), doi:10.3390/biom9080375.
Bilska-Wilkosz A, Górny M, Iciek M. Inactivation of Aldehyde Dehydrogenase By Disulfiram in the Presence and Absence of Lipoic Acid or Dihydrolipoic Acid: an in Vitro Study. Biomolecules. 2019 Aug 16;9(8) PubMed PMID: 31426424.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inactivation of Aldehyde Dehydrogenase by Disulfiram in the Presence and Absence of Lipoic Acid or Dihydrolipoic Acid: An in Vitro Study. AU - Bilska-Wilkosz,Anna, AU - Górny,Magdalena, AU - Iciek,Małgorzata, Y1 - 2019/08/16/ PY - 2019/06/27/received PY - 2019/08/02/revised PY - 2019/08/13/accepted PY - 2019/8/21/entrez PY - 2019/8/21/pubmed PY - 2019/8/21/medline KW - aldehyde dehydrogenase KW - dihydrolipoic acid KW - disulfiram KW - dithiothreitol KW - lipoic acid JF - Biomolecules JO - Biomolecules VL - 9 IS - 8 N2 - The inhibition of aldehyde dehydrogenase (ALDH) by disulfiram (DSF) in vitro can be prevented and/or reversed by dithiothreitol (DTT), which is a well-known low molecular weight non-physiological redox reagent commonly used in laboratory experiments. These observations inspired us to ask the question whether the inhibition of ALDH by DSF can be preserved or abolished also by dihydrolipoic acid (DHLA), which is the only currently known low molecular weight physiological dithiol in the body of humans and other animals. It can even be metaphorized that DHLA is an "endogenous DTT". Lipoic acid (LA) is the oxidized form of DHLA. We investigated the inactivation of ALDH derived from yeast and rat liver by DSF in the presence or absence of LA or DHLA. The results clearly show that DHLA is able both to restore and protect ALDH activity blocked by DSF. The proposed mechanism is discussed. SN - 2218-273X UR - https://www.unboundmedicine.com/medline/citation/31426424/Inactivation_of_Aldehyde_Dehydrogenase_by_Disulfiram_in_the_Presence_and_Absence_of_Lipoic_Acid_or_Dihydrolipoic_Acid:_An_in_Vitro_Study L2 - http://www.mdpi.com/resolver?pii=biom9080375 DB - PRIME DP - Unbound Medicine ER -