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Optimization of an Ultrasound-Assisted Extraction for Simultaneous Determination of Antioxidants in Sesame with Response Surface Methodology.

Abstract

Sesame is a nutritional agricultural product with medicinal properties. Accurate determination of micronutrients is important for the improvement of sesame quality and nutrition assessments. Our previous study showed that 10 antioxidants-d-homoproline, vitamin B2, coniferyl aldehyde, hesperidin, phloretin, N-acetyl-l-leucine, l-hyoscyamine, ferulic acid, 5-methoxypsoralen, and 8-methoxypsoralen-in sesame were potential characteristic nutrients in sesame. Herein, simultaneous detection of 10 different types of antioxidants was developed by using ultrasound-assisted extraction coupled with liquid chromatography-tandem mass spectrometry (UAE-LC-MS/MS) with the help of response surface methodology. The significant variables and levels were screened and optimized by combining the single factor experiment, Plackett-Burman test, and Box-Behnken design. The optimal conditions for extraction of target antioxidants in sesame were methanol solution of 75.0%, liquid-to-material ratio of 20:1 (mL/g), extraction temperature of 50 °C, extraction power of 410.0 W, extraction time of 65 min. The total yield of targets was 21.74 μg/g under the optimized conditions. The mobile phase used was 0.1% formic acid in acetonitrile and 0.1% formic acid in water, and the column was a Thermo Syncronis C18 reverse phase column (100 mm × 2.1 mm, 3 μm). All targets required only one injection and could be quickly separated and assayed within 7 min. The limits of detection and limits of quantification for these 10 nutritional compounds ranged from 0.01 to 0.11 µg/kg and from 0.04 to 0.34 µg/kg, respectively. The validation results indicated that the method had reasonable linearity (R2 ≥ 0.9990), good recoveries (71.1%-118.3%), satisfactory intra-day precision (≤9.6%) and inter-day precision (≤12.9%), and negligible matrix effects (≤13.8%). This simultaneous quantification method was accurate, fast, and robust for the assessment of sesame nutrition.

Authors+Show Affiliations

Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062 China. Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Wuhan 430062, China.Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062 China. Laboratory of Quality and Safety Risk Assessment for Oilseed Products (Wuhan), Ministry of Agriculture and Rural Affairs, Wuhan 430062, China. Quality Inspection and Test Center for Oilseed Products, Ministry of Agriculture and Rural Affairs, Wuhan 430062, China.Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062 China. Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Wuhan 430062, China.Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062 China. Quality Inspection and Test Center for Oilseed Products, Ministry of Agriculture and Rural Affairs, Wuhan 430062, China.Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062 China. Quality Inspection and Test Center for Oilseed Products, Ministry of Agriculture and Rural Affairs, Wuhan 430062, China.Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062 China. Quality Inspection and Test Center for Oilseed Products, Ministry of Agriculture and Rural Affairs, Wuhan 430062, China.Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062 China. Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture and Rural Affairs, Wuhan 430062, China.Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062 China. peiwuli@oilcrops.cn. Laboratory of Quality and Safety Risk Assessment for Oilseed Products (Wuhan), Ministry of Agriculture and Rural Affairs, Wuhan 430062, China. peiwuli@oilcrops.cn. Quality Inspection and Test Center for Oilseed Products, Ministry of Agriculture and Rural Affairs, Wuhan 430062, China. peiwuli@oilcrops.cn. Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture and Rural Affairs, Wuhan 430062, China. peiwuli@oilcrops.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31430991

Citation

Wang, Dandan, et al. "Optimization of an Ultrasound-Assisted Extraction for Simultaneous Determination of Antioxidants in Sesame With Response Surface Methodology." Antioxidants (Basel, Switzerland), vol. 8, no. 8, 2019.
Wang D, Zhang L, Xu Y, et al. Optimization of an Ultrasound-Assisted Extraction for Simultaneous Determination of Antioxidants in Sesame with Response Surface Methodology. Antioxidants (Basel). 2019;8(8).
Wang, D., Zhang, L., Xu, Y., Qi, X., Wang, X., Wang, X., ... Li, P. (2019). Optimization of an Ultrasound-Assisted Extraction for Simultaneous Determination of Antioxidants in Sesame with Response Surface Methodology. Antioxidants (Basel, Switzerland), 8(8), doi:10.3390/antiox8080321.
Wang D, et al. Optimization of an Ultrasound-Assisted Extraction for Simultaneous Determination of Antioxidants in Sesame With Response Surface Methodology. Antioxidants (Basel). 2019 Aug 19;8(8) PubMed PMID: 31430991.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Optimization of an Ultrasound-Assisted Extraction for Simultaneous Determination of Antioxidants in Sesame with Response Surface Methodology. AU - Wang,Dandan, AU - Zhang,Liangxiao, AU - Xu,Yueqing, AU - Qi,Xin, AU - Wang,Xuefang, AU - Wang,Xiupin, AU - Zhang,Qi, AU - Li,Peiwu, Y1 - 2019/08/19/ PY - 2019/08/02/received PY - 2019/08/09/revised PY - 2019/08/14/accepted PY - 2019/8/22/entrez PY - 2019/8/23/pubmed PY - 2019/8/23/medline KW - antioxidants KW - liquid chromatography-tandem mass spectrometry (LC-MS/MS) KW - response surface methodology KW - sesame KW - simultaneous detection KW - ultrasound-assisted extraction JF - Antioxidants (Basel, Switzerland) JO - Antioxidants (Basel) VL - 8 IS - 8 N2 - Sesame is a nutritional agricultural product with medicinal properties. Accurate determination of micronutrients is important for the improvement of sesame quality and nutrition assessments. Our previous study showed that 10 antioxidants-d-homoproline, vitamin B2, coniferyl aldehyde, hesperidin, phloretin, N-acetyl-l-leucine, l-hyoscyamine, ferulic acid, 5-methoxypsoralen, and 8-methoxypsoralen-in sesame were potential characteristic nutrients in sesame. Herein, simultaneous detection of 10 different types of antioxidants was developed by using ultrasound-assisted extraction coupled with liquid chromatography-tandem mass spectrometry (UAE-LC-MS/MS) with the help of response surface methodology. The significant variables and levels were screened and optimized by combining the single factor experiment, Plackett-Burman test, and Box-Behnken design. The optimal conditions for extraction of target antioxidants in sesame were methanol solution of 75.0%, liquid-to-material ratio of 20:1 (mL/g), extraction temperature of 50 °C, extraction power of 410.0 W, extraction time of 65 min. The total yield of targets was 21.74 μg/g under the optimized conditions. The mobile phase used was 0.1% formic acid in acetonitrile and 0.1% formic acid in water, and the column was a Thermo Syncronis C18 reverse phase column (100 mm × 2.1 mm, 3 μm). All targets required only one injection and could be quickly separated and assayed within 7 min. The limits of detection and limits of quantification for these 10 nutritional compounds ranged from 0.01 to 0.11 µg/kg and from 0.04 to 0.34 µg/kg, respectively. The validation results indicated that the method had reasonable linearity (R2 ≥ 0.9990), good recoveries (71.1%-118.3%), satisfactory intra-day precision (≤9.6%) and inter-day precision (≤12.9%), and negligible matrix effects (≤13.8%). This simultaneous quantification method was accurate, fast, and robust for the assessment of sesame nutrition. SN - 2076-3921 UR - https://www.unboundmedicine.com/medline/citation/31430991/Optimization_of_an_Ultrasound-Assisted_Extraction_for_Simultaneous_Determination_of_Antioxidants_in_Sesame_with_Response_Surface_Methodology L2 - http://www.mdpi.com/resolver?pii=antiox8080321 DB - PRIME DP - Unbound Medicine ER -