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Genetically encoded fluorescent biosensors illuminate kinase signaling in cancer.
J Biol Chem. 2019 10 04; 294(40):14814-14822.JB

Abstract

Protein kinase signaling networks stringently regulate cellular processes, such as proliferation, motility, and cell survival. These networks are also central to the evolution and progression of cancer. Accordingly, genetically encoded fluorescent biosensors capable of directly illuminating the spatiotemporal dynamics of kinase signaling in live cells are being increasingly used to investigate kinase signaling in cancer cells and tumor tissue sections. These biosensors enable visualization of biological processes and events directly in situ, preserving the native biological context and providing detailed insight into their localization and dynamics in cells. Herein, we first review common design strategies for kinase activity biosensors, including signaling targets, biosensor components, and fluorescent proteins involved. Subsequently, we discuss applications of biosensors to study the biology and management of cancer. These versatile molecular tools have been deployed to study oncogenic kinase signaling in living cells and image kinase activities in tumors or to decipher the mechanisms of anticancer drugs. We anticipate that the diversity and precision of genetically encoded biosensors will expand their use to further unravel the dysregulation of kinase signaling in cancer and the modes of actions of cancer-targeting drugs.

Authors+Show Affiliations

Department of Pharmacology, University of California, San Diego, La Jolla, California 92093-0702.Department of Pharmacology, University of California, San Diego, La Jolla, California 92093-0702.Department of Pharmacology, University of California, San Diego, La Jolla, California 92093-0702 jzhang32@ucsd.edu.

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Review

Language

eng

PubMed ID

31434714

Citation

Lin, Wei, et al. "Genetically Encoded Fluorescent Biosensors Illuminate Kinase Signaling in Cancer." The Journal of Biological Chemistry, vol. 294, no. 40, 2019, pp. 14814-14822.
Lin W, Mehta S, Zhang J. Genetically encoded fluorescent biosensors illuminate kinase signaling in cancer. J Biol Chem. 2019;294(40):14814-14822.
Lin, W., Mehta, S., & Zhang, J. (2019). Genetically encoded fluorescent biosensors illuminate kinase signaling in cancer. The Journal of Biological Chemistry, 294(40), 14814-14822. https://doi.org/10.1074/jbc.REV119.006177
Lin W, Mehta S, Zhang J. Genetically Encoded Fluorescent Biosensors Illuminate Kinase Signaling in Cancer. J Biol Chem. 2019 10 4;294(40):14814-14822. PubMed PMID: 31434714.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetically encoded fluorescent biosensors illuminate kinase signaling in cancer. AU - Lin,Wei, AU - Mehta,Sohum, AU - Zhang,Jin, Y1 - 2019/08/21/ PY - 2019/8/23/pubmed PY - 2020/5/7/medline PY - 2019/8/23/entrez KW - biosensor KW - cancer KW - cell signaling KW - fluorescence resonance energy transfer (FRET) KW - fluorescent protein KW - in vivo imaging KW - kinase signaling KW - phosphorylation KW - posttranslational modification SP - 14814 EP - 14822 JF - The Journal of biological chemistry JO - J. Biol. Chem. VL - 294 IS - 40 N2 - Protein kinase signaling networks stringently regulate cellular processes, such as proliferation, motility, and cell survival. These networks are also central to the evolution and progression of cancer. Accordingly, genetically encoded fluorescent biosensors capable of directly illuminating the spatiotemporal dynamics of kinase signaling in live cells are being increasingly used to investigate kinase signaling in cancer cells and tumor tissue sections. These biosensors enable visualization of biological processes and events directly in situ, preserving the native biological context and providing detailed insight into their localization and dynamics in cells. Herein, we first review common design strategies for kinase activity biosensors, including signaling targets, biosensor components, and fluorescent proteins involved. Subsequently, we discuss applications of biosensors to study the biology and management of cancer. These versatile molecular tools have been deployed to study oncogenic kinase signaling in living cells and image kinase activities in tumors or to decipher the mechanisms of anticancer drugs. We anticipate that the diversity and precision of genetically encoded biosensors will expand their use to further unravel the dysregulation of kinase signaling in cancer and the modes of actions of cancer-targeting drugs. SN - 1083-351X UR - https://www.unboundmedicine.com/medline/citation/31434714/Genetically_encoded_fluorescent_biosensors_illuminate_kinase_signaling_in_cancer_ L2 - http://www.jbc.org/cgi/pmidlookup?view=long&pmid=31434714 DB - PRIME DP - Unbound Medicine ER -