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Eco-friendly synthesis of silver nanoparticles using Senna alata bark extract and its antimicrobial mechanism through enhancement of bacterial membrane degradation.
J Microbiol Methods. 2019 10; 165:105692.JM

Abstract

Biological synthesis of nanomaterials has been increasingly gaining popularity due to its eco-friendly nature and cost-effectiveness. This study aimed to synthesize silver nanoparticles (AgNPs) using Senna alata bark extract as reducing and capping agents, and to evaluate their antimicrobial activities. AgNPs was characterized using UV-vis spectrophotometry, transmission electron microscopy, and Fourier transform infrared spectroscopy (FTIR). The formation of AgNPs was monitored by recording the surface plasmon resonance peak observed at 425 nm. High-resolution TEM images elucidated the formation of spherical AgNPs with an average diameter of 10-30 nm. Energy dispersive spectroscopy (EDS) revealed the presence of silver. The functional groups of biomolecules present in the extract and their interaction with AgNPs were identified through FTIR analysis. Biosynthesized AgNPs displayed antimicrobial activity against different microorganisms, including Gram-positive and Gram-negative bacteria as well as fungi, as indicated by the diameter of inhibition zones between 11.37 and 14.87 mm. Minimum inhibitory concentration of AgNPs for the tested microorganisms was in the range from 31.25 to 125 μg/mL. Potassium leakage is a primary indicator of membrane damage which is a significant mode of action of AgNPs against the tested microorganisms. The amount of potassium ions leaked from the microbial cells after 4 h contact time ranged between 0.97 and 3.05 ppm. Morphological changes were observed in all AgNPs-treated microorganisms. The green synthesized AgNPs with high antimicrobial activity has potential to be used in food packaging and biomedical research areas.

Authors+Show Affiliations

Natural Product Research Center of Excellence, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand.Natural Product Research Center of Excellence, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand; Department of Microbiology, Faculty of Science and Excellence Research Laboratory on Natural Products, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand.Center for Humanities and Sciences, BioNanocomposite Research Center, Department of Food and Nutrition, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Republic of Korea. Electronic address: shivbiotech@gmail.com.Natural Product Research Center of Excellence, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand; Department of Microbiology, Faculty of Science and Excellence Research Laboratory on Natural Products, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand. Electronic address: supayang.v@psu.ac.th.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31437555

Citation

Ontong, Julalak C., et al. "Eco-friendly Synthesis of Silver Nanoparticles Using Senna Alata Bark Extract and Its Antimicrobial Mechanism Through Enhancement of Bacterial Membrane Degradation." Journal of Microbiological Methods, vol. 165, 2019, p. 105692.
Ontong JC, Paosen S, Shankar S, et al. Eco-friendly synthesis of silver nanoparticles using Senna alata bark extract and its antimicrobial mechanism through enhancement of bacterial membrane degradation. J Microbiol Methods. 2019;165:105692.
Ontong, J. C., Paosen, S., Shankar, S., & Voravuthikunchai, S. P. (2019). Eco-friendly synthesis of silver nanoparticles using Senna alata bark extract and its antimicrobial mechanism through enhancement of bacterial membrane degradation. Journal of Microbiological Methods, 165, 105692. https://doi.org/10.1016/j.mimet.2019.105692
Ontong JC, et al. Eco-friendly Synthesis of Silver Nanoparticles Using Senna Alata Bark Extract and Its Antimicrobial Mechanism Through Enhancement of Bacterial Membrane Degradation. J Microbiol Methods. 2019;165:105692. PubMed PMID: 31437555.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Eco-friendly synthesis of silver nanoparticles using Senna alata bark extract and its antimicrobial mechanism through enhancement of bacterial membrane degradation. AU - Ontong,Julalak C, AU - Paosen,Supakit, AU - Shankar,Shiv, AU - Voravuthikunchai,Supayang P, Y1 - 2019/08/19/ PY - 2019/07/08/received PY - 2019/08/14/revised PY - 2019/08/16/accepted PY - 2019/8/23/pubmed PY - 2020/7/23/medline PY - 2019/8/23/entrez KW - Antimicrobial activity KW - Green synthesis KW - Potassium ion leakage KW - Senna alata extract KW - Silver nanoparticles SP - 105692 EP - 105692 JF - Journal of microbiological methods JO - J Microbiol Methods VL - 165 N2 - Biological synthesis of nanomaterials has been increasingly gaining popularity due to its eco-friendly nature and cost-effectiveness. This study aimed to synthesize silver nanoparticles (AgNPs) using Senna alata bark extract as reducing and capping agents, and to evaluate their antimicrobial activities. AgNPs was characterized using UV-vis spectrophotometry, transmission electron microscopy, and Fourier transform infrared spectroscopy (FTIR). The formation of AgNPs was monitored by recording the surface plasmon resonance peak observed at 425 nm. High-resolution TEM images elucidated the formation of spherical AgNPs with an average diameter of 10-30 nm. Energy dispersive spectroscopy (EDS) revealed the presence of silver. The functional groups of biomolecules present in the extract and their interaction with AgNPs were identified through FTIR analysis. Biosynthesized AgNPs displayed antimicrobial activity against different microorganisms, including Gram-positive and Gram-negative bacteria as well as fungi, as indicated by the diameter of inhibition zones between 11.37 and 14.87 mm. Minimum inhibitory concentration of AgNPs for the tested microorganisms was in the range from 31.25 to 125 μg/mL. Potassium leakage is a primary indicator of membrane damage which is a significant mode of action of AgNPs against the tested microorganisms. The amount of potassium ions leaked from the microbial cells after 4 h contact time ranged between 0.97 and 3.05 ppm. Morphological changes were observed in all AgNPs-treated microorganisms. The green synthesized AgNPs with high antimicrobial activity has potential to be used in food packaging and biomedical research areas. SN - 1872-8359 UR - https://www.unboundmedicine.com/medline/citation/31437555/Eco_friendly_synthesis_of_silver_nanoparticles_using_Senna_alata_bark_extract_and_its_antimicrobial_mechanism_through_enhancement_of_bacterial_membrane_degradation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0167-7012(19)30584-6 DB - PRIME DP - Unbound Medicine ER -