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Purified human and recombinant murine interleukin-1 alpha induced accumulation of inflammatory peritoneal neutrophils and mononuclear phagocytes: possible contributions to antibacterial resistance.
Microb Pathog. 1987 Nov; 3(5):377-86.MP

Abstract

Interleukin-1 (IL-1) mediates a number of proinflammatory biological responses that are thought to contribute to antibacterial resistance. In the present study we examined the ability of IL-1 to recruit inflammatory neutrophils and mononuclear phagocytes in vivo; a function that has been reported to be closely related to antibacterial resistance. Intraperitoneal injection of small amounts (1-10 LAF units) of purified human or recombinant murine IL-1 alpha (rIL-alpha) resulted in an increased influx of inflammatory neutrophils into the peritoneal cavity that peaked at 4-14 h after IL-1 injection. A small but consistent increase in peritoneal macrophages also was observed at 72 h after rIL-1 alpha injection. The ability of rIL-1 alpha to induce neutrophil accumulation was uninfluenced by polymyxin B, was sensitive to heat treatment (100 degrees C for 1 h), and was observed after i.p. injection into LPS-nonresponsive C3H/HeJ mice. These three lines of evidence suggested that contaminating LPS did not contribute substantially to rIL-1 alpha induced accumulation of neutrophils. Treatment of mice with indomethacin or nordihydroguaiaretic acid did not abrogate rIL-1 alpha induced neutrophil accumulation. Mice injected i.p. with increasing amounts of rIL-1 alpha demonstrated a corresponding enhancement of their resistance to an i.p. L. monocytogenes challenge 4 h later, thus suggesting that IL-1 mediated inflammatory phagocyte accumulation may contribute in part to nonspecific antibacterial resistance.

Authors+Show Affiliations

Department of Pathobiological Sciences, University of Wisconsin, School of Veterinary Medicine, Madison 53706.No affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

3143888

Citation

Czuprynski, C J., and J F. Brown. "Purified Human and Recombinant Murine Interleukin-1 Alpha Induced Accumulation of Inflammatory Peritoneal Neutrophils and Mononuclear Phagocytes: Possible Contributions to Antibacterial Resistance." Microbial Pathogenesis, vol. 3, no. 5, 1987, pp. 377-86.
Czuprynski CJ, Brown JF. Purified human and recombinant murine interleukin-1 alpha induced accumulation of inflammatory peritoneal neutrophils and mononuclear phagocytes: possible contributions to antibacterial resistance. Microb Pathog. 1987;3(5):377-86.
Czuprynski, C. J., & Brown, J. F. (1987). Purified human and recombinant murine interleukin-1 alpha induced accumulation of inflammatory peritoneal neutrophils and mononuclear phagocytes: possible contributions to antibacterial resistance. Microbial Pathogenesis, 3(5), 377-86.
Czuprynski CJ, Brown JF. Purified Human and Recombinant Murine Interleukin-1 Alpha Induced Accumulation of Inflammatory Peritoneal Neutrophils and Mononuclear Phagocytes: Possible Contributions to Antibacterial Resistance. Microb Pathog. 1987;3(5):377-86. PubMed PMID: 3143888.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Purified human and recombinant murine interleukin-1 alpha induced accumulation of inflammatory peritoneal neutrophils and mononuclear phagocytes: possible contributions to antibacterial resistance. AU - Czuprynski,C J, AU - Brown,J F, PY - 1987/11/1/pubmed PY - 1987/11/1/medline PY - 1987/11/1/entrez SP - 377 EP - 86 JF - Microbial pathogenesis JO - Microb Pathog VL - 3 IS - 5 N2 - Interleukin-1 (IL-1) mediates a number of proinflammatory biological responses that are thought to contribute to antibacterial resistance. In the present study we examined the ability of IL-1 to recruit inflammatory neutrophils and mononuclear phagocytes in vivo; a function that has been reported to be closely related to antibacterial resistance. Intraperitoneal injection of small amounts (1-10 LAF units) of purified human or recombinant murine IL-1 alpha (rIL-alpha) resulted in an increased influx of inflammatory neutrophils into the peritoneal cavity that peaked at 4-14 h after IL-1 injection. A small but consistent increase in peritoneal macrophages also was observed at 72 h after rIL-1 alpha injection. The ability of rIL-1 alpha to induce neutrophil accumulation was uninfluenced by polymyxin B, was sensitive to heat treatment (100 degrees C for 1 h), and was observed after i.p. injection into LPS-nonresponsive C3H/HeJ mice. These three lines of evidence suggested that contaminating LPS did not contribute substantially to rIL-1 alpha induced accumulation of neutrophils. Treatment of mice with indomethacin or nordihydroguaiaretic acid did not abrogate rIL-1 alpha induced neutrophil accumulation. Mice injected i.p. with increasing amounts of rIL-1 alpha demonstrated a corresponding enhancement of their resistance to an i.p. L. monocytogenes challenge 4 h later, thus suggesting that IL-1 mediated inflammatory phagocyte accumulation may contribute in part to nonspecific antibacterial resistance. SN - 0882-4010 UR - https://www.unboundmedicine.com/medline/citation/3143888/Purified_human_and_recombinant_murine_interleukin_1_alpha_induced_accumulation_of_inflammatory_peritoneal_neutrophils_and_mononuclear_phagocytes:_possible_contributions_to_antibacterial_resistance_ DB - PRIME DP - Unbound Medicine ER -